Genes from pUM505 plasmid contribute to Pseudomonas aeruginosa virulence

Rodríguez-Andrade, E.; Hernández-Ramírez, Karen C.; Díaz-Pérez, Sharel Pamela; Díaz-Magaña, Amada; Chávez-Moctezuma, Martha P.; Meza-Carmen, Víctor; Ortiz-Alvarado, Rafael; Cervantes, Carlos; Ramírez-Díaz, Martha I.

doi:10.1007/s10482-015-0642-9

Cited by 10 publications

(6 citation statements)

References 21 publications

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“…The pUM505 plasmid is a conjugative plasmid isolated from a clinical isolate of P. aeruginosa (9). pUM505 carries different genes, which encode proteins involved in metal resistance, DNA repair, virulence, and plasmid stability (10)(11)(12)(13). Additionally, pUM505 conferred CIP resistance on P. aeruginosa PU21 (13), suggesting that pUM505 contains genes responsible for quinolone resistance.…”

Section: Discussionmentioning

confidence: 99%

“…pUM505 is a self-conjugating 123-kbp plasmid isolated from a clinical Pseudomonas aeruginosa isolate (9). This plasmid carries several adaptive genes, including the umuD gene (encoding a transcriptional regulator of the SOS response) (10), genes involved in Pseudomonas virulence (11,12), and genes that increase plasmid stability (12). The aim of this work was to identify and study the product of the orf131 (renamed crpP) gene on pUM505, which confers resistance to CIP.…”

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confidence: 99%

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CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

Chávez-Jacobo

Hernández-Ramírez

Romo-Rodríguez

et al. 2018

Antimicrob Agents Chemother

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The pUM505 plasmid, isolated from a clinical isolate, confers resistance to ciprofloxacin (CIP) when transferred into the standard strain PAO1. CIP is an antibiotic of the quinolone family that is used to treat infections. analysis, performed to identify CIP resistance genes, revealed that the 65-amino-acid product encoded by the gene in pUM505 displays 40% amino acid identity to the aminoglycoside phosphotransferase (an enzyme that phosphorylates and inactivates aminoglycoside antibiotics). We cloned (renamed, for iprofloxacinesistance rotein,lasmid encoded) into the pUCP20 shuttle vector. The resulting recombinant plasmid, pUC-, conferred resistance to CIP on strain J53-3, suggesting that this gene encodes a protein involved in CIP resistance. Using coupled enzymatic analysis, we determined that the activity of CrpP on CIP is ATP dependent, while little activity against norfloxacin was detected, suggesting that CIP may undergo phosphorylation. Using a recombinant His-tagged CrpP protein and liquid chromatography-tandem mass spectrometry, we also showed that CIP was phosphorylated prior to its degradation. Thus, our findings demonstrate that CrpP, encoded on the pUM505 plasmid, represents a new mechanism of CIP resistance in, which involves phosphorylation of the antibiotic.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

Chávez-Jacobo

Hernández-Ramírez

Romo-Rodríguez

et al. 2018

Antimicrob Agents Chemother

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“…To assess the virulence of M . circinelloides strains, we used methods described previously [18, 54] with some modifications. Briefly, each group consisting of eight male BALB/c mice (12–16 weeks old, weighing ~20 g, obtained from CINVESTAV, Zacatenco.…”

Section: Methodsmentioning

confidence: 99%

Heterotrimeric G-alpha subunits Gpa11 and Gpa12 define a transduction pathway that control spore size and virulence in Mucor circinelloides

et al. 2019

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Mucor circinelloides is one of the causal agents of mucormycosis, an emerging and high mortality rate fungal infection produced by asexual spores (sporangiospores) of fungi that belong to the order Mucorales. M. circinelloides has served as a model genetic system to understand the virulence mechanism of this infection. Although the G-protein signaling cascade plays crucial roles in virulence in many pathogenic fungi, its roles in Mucorales are yet to be elucidated. Previous study found that sporangiospore size and calcineurin are related to the virulence in Mucor, in which larger spores are more virulent in an animal mucormycosis model and loss of a calcineurin A catalytic subunit CnaA results in larger spore production and virulent phenotype. The M. circinelloides genome is known to harbor twelve gpa (gpa1 to gpa12) encoding G-protein alpha subunits and the transcripts of the gpa11 and gpa12 comprise nearly 72% of all twelve gpa genes transcript in spores. In this study we demonstrated that loss of function of Gpa11 and Gpa12 led to larger spore size associated with reduced activation of the calcineurin pathway. Interestingly, we found lower levels of the cnaA mRNAs in sporangiospores from the Δgpa12 and double Δgpa11/Δgpa12 mutant strains compared to wild-type and the ΔcnaA mutant had significantly lower gpa11 and gpa12 mRNA levels compared to wild-type. However, in contrast to the high virulence showed by the large spores of ΔcnaA, the spores from Δgpa11/Δgpa12 were avirulent and produced lower tissue invasion and cellular damage, suggesting that the gpa11 and gpa12 define a signal pathway with two branches. One of the branches controls spore size through regulation of calcineurin pathway, whereas virulences is controlled by an independent pathway. This virulence-related regulatory pathway could control the expression of genes involved in cellular responses important for virulence, since sporangiospores of Δgpa11/Δgpa12 were less resistant to oxidative stress and phagocytosis by macrophages than the ΔcnaA and wild-type strains. The characterization of this pathway could contribute to decipher the signals and mechanism used by Mucorales to produce mucormycosis.

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“…Moreover, it was already shown that the TA modules can be used for the development of male sterile plants for containment of transgenic plants or for hybrid seed production [ 40 , 41 ]. Furthermore, despite the evolutionary distance, plants can be successfully used as experimental models for human microbial pathogens [ 42 , 43 , 44 ]. The bacterium species examined in this study, Dickeya dadantii 3937, is considered as a model organism for bacterial plant pathogens of the Dickeya genus, and therefore, a majority of genetic and virulence studies were performed on this species.…”

Section: Discussionmentioning

confidence: 99%

Identification of Three Type II Toxin-Antitoxin Systems in Model Bacterial Plant Pathogen Dickeya dadantii 3937

Boss

Górniak

Lewańczyk

et al. 2021

IJMS

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Type II toxin-antitoxin (TA) systems are genetic elements usually encoding two proteins: a stable toxin and an antitoxin, which binds the toxin and neutralizes its toxic effect. The disturbance in the intracellular toxin and antitoxin ratio typically leads to inhibition of bacterial growth or bacterial cell death. Despite the fact that TA modules are widespread in bacteria and archaea, the biological role of these systems is ambiguous. Nevertheless, a number of studies suggests that the TA modules are engaged in such important processes as biofilm formation, stress response or virulence and maintenance of mobile genetic elements. The Dickeya dadantii 3937 strain serves as a model for pathogens causing the soft-rot disease in a wide range of angiosperm plants. Until now, several chromosome-encoded type II TA systems were identified in silico in the genome of this economically important bacterium, however so far only one of them was experimentally validated. In this study, we investigated three putative type II TA systems in D. dadantii 3937: ccdAB2Dda, phd-docDda and dhiTA, which represents a novel toxin/antitoxin superfamily. We provide an experimental proof for their functionality in vivo both in D. dadantii and Escherichia coli. Finally, we examined the prevalence of those systems across the Pectobacteriaceae family by a phylogenetic analysis.

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Genes from pUM505 plasmid contribute to Pseudomonas aeruginosa virulence

Cited by 10 publications

References 21 publications

CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

CrpP Is a Novel Ciprofloxacin-Modifying Enzyme Encoded by the Pseudomonas aeruginosa pUM505 Plasmid

Heterotrimeric G-alpha subunits Gpa11 and Gpa12 define a transduction pathway that control spore size and virulence in Mucor circinelloides

Identification of Three Type II Toxin-Antitoxin Systems in Model Bacterial Plant Pathogen Dickeya dadantii 3937

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