Genes from<i>Debaryomyces hansenii</i>increase salt tolerance in<i>Saccharomyces cerevisiae</i>W303

Prista, Catarina; Soeiro, Ana; Vesely, Paul; Almagro, Anabel; Ramos, José; Loureiro-Dias, Maria C.

doi:10.1111/j.1567-1364.2002.tb00079.x

Cited by 12 publications

(7 citation statements)

References 37 publications

(48 reference statements)

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“…More recently, it has been possible to construct strains of S. cerevisiae capable of growth at higher sodium concentration and/or at lower potassium concentration by transformation with a gene library from D. hansenii [16,17]. This observation demonstrates that genetic material from D. hansenii accounts for increased salt tolerance and that this material can be transferred to S. cerevisiae , conferring a different phenotype.…”

Section: General Physiologymentioning

confidence: 99%

Mechanisms underlying the halotolerant way of

Prista¹,

Loureiro-Dias²,

Montiel³

et al. 2005

FEMS Yeast Research

125

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The yeast Debaryomyces hansenii is usually found in salty environments such as the sea and salted food. It is capable of accumulating sodium without being intoxicated even when potassium is present at low concentration in the environment. In addition, sodium improves growth and protects D. hansenii in the presence of additional stress factors such as high temperature and extreme pH. An array of advantageous factors, as compared with Saccharomyces cerevisiae, is putatively involved in the increased halotolerance of D. hansenii: glycerol, the main compatible solute, is kept inside the cell by an active glycerol-Na+ symporter; potassium uptake is not inhibited by sodium; sodium protein targets in D. hansenii seem to be more resistant. The whole genome of D. hansenii has been sequenced and is now available at http://cbi.labri.fr/Genolevures/ and, so far, no genes specifically responsible for the halotolerant behaviour of D. hansenii have been found.

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Section: General Physiologymentioning

confidence: 99%

Mechanisms underlying the halotolerant way of

Prista¹,

Loureiro-Dias²,

Montiel³

et al. 2005

FEMS Yeast Research

125

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“…Wild-type yeast strain W303-1A was transformed with a genomic multicopy library from D. hansenii strain PYCC2968 (CBS767), which was constructed as described previously (38). Transformants were grown in complete minimal medium plates lacking uracil and replica plated onto the same plates containing 20 mM N-Tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid and adjusted to pH 7.7 with arginine.…”

Section: Methodsmentioning

confidence: 99%

Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii

et al. 2006

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The yeast Debaryomyces hansenii has a remarkable capacity to proliferate in salty and alkaline environments such as seawater. A screen for D. hansenii genes able to confer increased tolerance to high pH when overexpressed in Saccharomyces cerevisiae yielded a single gene, named here DhGZF3, encoding a putative negative GATA transcription factor related to S. cerevisiae Dal80 and Gzf3. Overexpression of this gene in wild-type S. cerevisiae increased caffeine and rapamycin tolerance, blocked growth in low glucose concentrations and nonfermentable carbon sources, and resulted in lithium-and sodium-sensitive cells. Sensitivity to salt could be attributed to a reduced cation efflux, most likely because of a decrease in expression of the ENA1 Na ؉ -ATPase gene. Overexpression of DhGZF3 did not affect cell growth in a gat1 mutant but was lethal in the absence of Gln3. These are positive factors that oppose both Gzf3 and Dal80. Genome-wide transcriptional profiling of wild-type cells overexpressing DhGZF3 shows decreased expression of a number of genes that are usually induced in poor nitrogen sources. In addition, the entire pathway leading to Lys biosynthesis was repressed, probably as a result of a decrease in the expression of the specific Lys14 transcription factor. In conclusion, our results demonstrate that DhGzf3 can play a role as a negative GATA transcription factor when expressed in S. cerevisiae and that it most probably represents the only member of this family in D. hansenii. These findings also point to the GATA transcription factors as relevant elements for alkaline-pH tolerance.Adaptation to conditions of high-salt stress is a biological process of the utmost importance in agriculture and biotechnology due to the increased salinization of irrigated lands. Debaryomyces hansenii is an ascomycetous salt-and high-pHtolerant yeast found in seawaters and salty food. This yeast is becoming a model for the study of salt tolerance mechanisms in eukaryotic cells (37), and this research will be boosted by the recent release of its complete genomic sequence (21). Although the halotolerant characteristics of this organism were defined a long time ago (33,34,36), the molecular basis for the halotolerant phenotype is still obscure. D. hansenii encodes orthologs to most components that have been found to be relevant for salt tolerance in other fungi, particularly in the model yeast Saccharomyces cerevisiae. Examples are the DhHOG1 mitogen-activated protein kinase, the DhHAL2 nucleotidase, and the DhENA1 and DhENA2 sodium ATPase pumps (2, 4, 5). In most cases, these gene products have proven to be functional for complementing the equivalent S. cerevisiae mutants, although they did not confer a particularly strong salt-tolerant phenotype even when overexpressed. However, the fact that D. hansenii is not an easily tractable organism from a genetic point of view and the lack of molecular tools to approach its study have been important disadvantages in attempts to define the basis for its unusual salt tolerance.The natural ...

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“…Therefore, in order to exploit salt‐tolerant fungal species as models for salt resistance in plants, it is important to study the role of oxidative stress in these eukaryotic microorganisms. The extremely halotolerant yeasts Hortaea werneckii , Debaryomyces hansenii and Candida versatilis ( C. halophila ) have been proposed as models for studying the molecular and physiological basis of salt tolerance in eukaryotes (Petrovič et al , 2002; Prista et al , 2002; Silva‐Graça & Lucas, 2003; Silva‐Graça et al , 2003). Because of the high energy demands of thriving in such environments (Oren, 1999), oxidative stress originating from intensive mitochondrial respiration (Gille & Sigler, 1995) could pose a further threat to the survival of aerobic microorganisms living in high‐salinity environments.…”

Section: Introductionmentioning

confidence: 99%

Role of oxidative stress in the extremely salt-tolerant yeastHortaea werneckii

Petrovič

2006

FEMS Yeast Research

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Eukaryotic halotolerant microorganisms are important as model organisms to understand the general mechanisms of resistance to environmental salinity. The ability of the extremely halotolerant black yeast Hortaea werneckii to combat oxidative stress was addressed, using hydrogen peroxide to generate the reactive oxygen species. Increasing environmental salinity was found to have no effect on its high ability to degrade hydrogen peroxide but resulted in a decrease in viability in response to externally added hydrogen peroxide, suggesting that the latter property determines the upper limit of the salt tolerance of H. werneckii. A refinement of the model of adaptation of H. werneckii to high‐salinity environments is proposed.

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Genes fromDebaryomyces hanseniiincrease salt tolerance inSaccharomyces cerevisiaeW303

Cited by 12 publications

References 37 publications

Mechanisms underlying the halotolerant way of

Mechanisms underlying the halotolerant way of

Heterologous Expression Implicates a GATA Factor in Regulation of Nitrogen Metabolic Genes and Ion Homeostasis in the Halotolerant Yeast Debaryomyces hansenii

Role of oxidative stress in the extremely salt-tolerant yeastHortaea werneckii

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