Genes for immunoglobulin heavy chain and serum prealbumin protein are linked in mouse

Taylor, Benjamin A.; Bailey, Donald W.; Cherry, Marianna; Riblet, Roy; Weigert, Martin

doi:10.1038/256644a0

Cited by 111 publications

(41 citation statements)

References 22 publications

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“…Since 5 among 14 recombinant inbred strains had a recombination between the Dao-1 and Pgm-1 loci (Hilgers and Arends, 1985), the recombination frequency between these loci can be calculated as 19.2±14.9% according to Taylor et al (1975). The value is in good agreement with that obtained in the present experiments.…”

Section: Resultssupporting

confidence: 82%

Linkage of the Dao-1 gene for D-amino-acid oxidase to the pgm-1 gene for phosphoglucomutase-1 on the mouse chromosome 5.

Konno

Niwa

Yasumura

1989

The Japanese journal of genetics

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Linkage of the Dao-1 gene controlling D-amino-acid oxidase to the Pgm-1 gene controlling phosphoglucomutase-1 on the mouse chromosome 5 was examined. Mutant ddY/DAOmice carrying a null Dao-1 ' allele had a Pgm-1 a allele. Mutant mice were crossed to C3H/HeN and NZB/Kl mice carrying the Dao-1 + and Pgm-1 b alleles.The hybrid F1 mice were backcrossed to the ddY/DAOand their progeny were examined for alleles for D-amino-acid oxidase and phosphoglucomutase-1.In both backcrosses, the progeny with recombinant-type combinations of the alleles were significantly less than the progeny with the parental-type combinations, indicating the linkage of the Dao-1 and Pgm-1 genes. The recombination frequency between these loci was estimated to be 19.8±4.0%.

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Section: Resultssupporting

confidence: 82%

Linkage of the Dao-1 gene for D-amino-acid oxidase to the pgm-1 gene for phosphoglucomutase-1 on the mouse chromosome 5.

Konno

Niwa

Yasumura

1989

The Japanese journal of genetics

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“…A comparison of the hybridization and karyotypic data permit us to exclude certain chromosomes as sites for C-region genes and thereby identify particular chromosomes as possible sites of constant-region genes (Table 3). At least one gene on each mouse chromosome has previously been tested for linkage for CH and Pre, a gene 10-12 centimorgans from CH, and so far, close linkage has not been observed (13)(14)(15). These genetic data do not exclude CH's being on any of these chromosomes but suggest that the CH genes are not located near the tested markers or that they are located near a recombinational "hot spot."…”

Section: Chromosomal Composition Of Hybrid Cell Linesmentioning

confidence: 99%

Chromosomal locations of mouse immunoglobulin genes.

Valbuena¹,

Marcu²,

Croce³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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The A comparison of karyotypic data with hybridization data has limited the possible locations of the Ig genes to only a few chromosomes.In the mouse, as in other mammals, the chromosomal locations of the genes coding for antibody heavy and light chain structures have not been clearly identified. From genetic studies it has been inferred that these genes are located on different autosomes (1), and it has been suggested on the basis of a variable region marker that the mouse K locus may be on chromosome 6 (2). However, owing to the lack of a demonstrable linkage between heavy or light chain constant-region genes and other loci and to the possible existence of regulatory loci affecting the expression of the immunoglobulin structural genes (3, 4), a more definitive study is called for. We have therefore attempted to solve this problem by use of the molecular hybridization techniques. In these experiments, cDNA probes representing the constant regions of mouse K and AI light chain mRNAs and a, 72h and M heavy chain mRNAs were annealed to a large excess of DNA from a series of mouse-human hybrid cell lines that are deficient for various mouse chromosomes (5). A similar approach using hybrids segregating human chromosomes has shown that the structural gene for human a-globin is on chromosome 16 (6). The results of our studies demonstrate clearly that genes CA, CK, and CH are autosomal and not linked. Moreover, a comparison of karyotypic and hybridization data has limited the possible locations of these genes to only a few chromosomes. Interestingly, our results indicate that the chromosome containing the CK gene is probably not chromosome 6. 55-14, 55-54, and 55-91 were derived from three independent fusions of HT-1080-6TG human fibrosarcoma cells with BALB/c mouse peritoneal macrophages; the hybrid line 55-84 was derived from a fusion of HT-1080-6TG cells with OTT6050 mouse teratocarcinoma cells (5). The F numbers refer to different flasks in which hybrids were formed following the initial fusion event, and Cl numbers refer to different clonal isolates from the initial hybrid cell cultures. Line VII is an early sample of the 55-14 F7 culture that was kept frozen until its cultivation for the present series of experiments. Karyotype analyses were carried out by using the trypsin-Giemsa banding technique (5). MATERIALS AND METHODSDNAcDNA Annealing Experiments. The cDNA probes were synthesized from highly purified heavy and light chain mRNAs isolated from various mouse plasmacytomas. The tumors used as sources of the particular mRNAs were: AI, H2020

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“…The B x D RI strains were derived by crossing the C57BL/6J (B) and DBA/2J (D) strains, followed by systematic inbreeding of the progeny, beginning with chosen pairs from the F2 generation (16,17). These strains are now highly inbred and have been used extensively for gene mapping (3,18,19). The probability of recombination occurring between any two loci in the course of inbreeding is related to the map distance between them (16).…”

mentioning

confidence: 99%

Chromosomal mapping of the mink cell focus-inducing and xenotropic env gene family in the mouse.

Blatt¹,

Mileham²,

Haas³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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Chromosomal locations of members of the xenotropic-related env gene family in the mouse genome have been determined. Endonuclease restriction site polymorphisms detected by molecular hybridization were used to.study the inheritance of mink cell-focus inducing and xenotropic env gene-related sequences in recombinant inbred strains of mice. Some of the endogenous env sequences, appear to be eloselylinked to genes determining leukemia virus induction and to genes involved in the immune response, such as the heavy and light chains of the immunoglobulin molecules or allotypic determinants on B and T lymphocytes. The use of probes that detect restriction fragment length polymorphisms in a small family of dispersed sequences promises to yield a large number of markers that can be used together with recombinant inbred strains for efficient mapping of the mouse genome.

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Genes for immunoglobulin heavy chain and serum prealbumin protein are linked in mouse

Cited by 111 publications

References 22 publications

Linkage of the Dao-1 gene for D-amino-acid oxidase to the pgm-1 gene for phosphoglucomutase-1 on the mouse chromosome 5.

Linkage of the Dao-1 gene for D-amino-acid oxidase to the pgm-1 gene for phosphoglucomutase-1 on the mouse chromosome 5.

Chromosomal locations of mouse immunoglobulin genes.

Chromosomal mapping of the mink cell focus-inducing and xenotropic env gene family in the mouse.

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