Genes and Molecular Pathways of the Osteogenic Process

Lattanzi, Wanda; Bernardini, Camilla

doi:10.5772/34022

Cited by 14 publications

(10 citation statements)

References 205 publications

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“…Indeed, the most promising field for ASCs application is represented by bone reconstruction/regeneration [ 38 , 51 ]. Bones are dynamic organs, undergoing continuous remodeling to maintain tissue homeostasis, modify shape and morphology, and repair fractures [ 52 ]. The therapeutic options clinically available are currently restricted to allografts, microvascular bone, and osteomyocutaneous flaps taken from an autologous donor site, and bone distraction for reconstructive purposes [ 53 – 55 ].…”

Section: Osteogenic Potential Of Ascs and Their Role In Bone Regenmentioning

confidence: 99%

Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

Barba

Cicione

Bernardini

et al. 2013

BioMed Research International

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Adipose tissue represents a hot topic in regenerative medicine because of the tissue source abundance, the relatively easy retrieval, and the inherent biological properties of mesenchymal stem cells residing in its stroma. Adipose-derived mesenchymal stem cells (ASCs) are indeed multipotent somatic stem cells exhibiting growth kinetics and plasticity, proved to induce efficient tissue regeneration in several biomedical applications. A defined consensus for their isolation, classification, and characterization has been very recently achieved. In particular, bone tissue reconstruction and regeneration based on ASCs has emerged as a promising approach to restore structure and function of bone compromised by injury or disease. ASCs have been used in combination with osteoinductive biomaterial and/or osteogenic molecules, in either static or dynamic culture systems, to improve bone regeneration in several animal models. To date, few clinical trials on ASC-based bone reconstruction have been concluded and proved effective. The aim of this review is to dissect the state of the art on ASC use in bone regenerative applications in the attempt to provide a comprehensive coverage of the topics, from the basic laboratory to recent clinical applications.

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Section: Osteogenic Potential Of Ascs and Their Role In Bone Regenmentioning

confidence: 99%

Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

Barba

Cicione

Bernardini

et al. 2013

BioMed Research International

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“…KAAS retrieved 6808 KO identifiers for 10 520 genes (26.25% of 40 066), and mapping of KO identifiers generated 337 KEGG pathway maps composed of several functional categories (Table 3). We reconstructed some of these pathways to obtain an overview of the completeness of the assembled transcriptome and to ascertain the representation of some important signalling pathways involved in regulation of skeletal development (reviewed by Karsenty 2008;Lefebvre & Bhattaram 2010;Lattanzi & Bernardini 2012) and innate immunity of fishes (reviewed by Rebl et al 2010). For example, the transforming growth factor-beta (TGF-b, Fig.…”

Section: Resultsmentioning

confidence: 99%

A comprehensive transcriptome of early development in yellowtail kingfish (Seriola lalandi)

Patel

Dettleff

Hernandez

et al. 2015

Molecular Ecology Resources

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Seriola lalandi is an ecologically and economically important species that is globally distributed in temperate and subtropical marine waters. The aim of this study was to identify large numbers of genic single nucleotide polymorphisms (SNPs) and differential gene expression (DGE) related to the early development of normal and deformed S. lalandi larvae using high-throughput RNA-seq data. A de novo assembly of reads generated 40,066 genes ranging from 300 bases to 64,799 bases with an N90 of 788 bases. Homology search and protein signature recognition assigned gene ontology (GO) terms to a total of 15,744 (39.34%) genes. A search against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG) retrieved 6808 KEGG orthology (KO) identifiers for 10,520 genes (26.25%), and mapping of KO identifiers generated 337 KEGG pathways. Comparisons of annotated genes revealed that 1262 genes were downregulated and 1047 genes were upregulated in the deformed larvae group compared to the normal group of larvae. Additionally, we identified 6989 high-quality SNPs from the assembled transcriptome. These putative SNPs contain 4415 transitions and 2574 transversions, which will be useful for further ecological studies of S. lalandi. This is the first study to use a global transcriptomic approach in S. lalandi, and the resources generated can be used further for investigation of gene expression of marine teleosts to investigate larval developmental biology. The results of the GO enrichment analysis highlight the crucial role of the extracellular matrix in normal skeleton development, which could be important for future studies of skeletal deformities in S. lalandi and other marine species.

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“…Communication between osteocytes and osteoclasts can be mediated through the Notch signaling pathway (Engin and Lee, 2010). Notch signaling is also involved in chondrogenic differentiation, although the role during chondro / osteogenesis has not been yet elucidated (Lattanzi and Bernardini, 2012). Moreover, this pathway is an essential sign for the debut of formation and maturation process of osteoclasts.…”

Section: Notch Signaling Pathwaymentioning

confidence: 99%

An overview on osteogenic differentiation process: Minimum essential information for bone tissue engineering

Șelaru¹,

Samoilă²,

Dinescu³

et al. 2018

Rev. Biol. Biomed. Sci.

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Bone tissue engineering is a new and modern strategy that serves for repairing major bone defects. Three essential elements are involved in bone tissue engineering: a scaffold, a cell source that can convert into bone cells and growth factors. In order to develop new and original biomaterials with high compatibility it is necessary to fully understand osteoblastogenesis. Osteoblasts and osteoclasts are the two types of cells involved in the formation of bone tissue. Osteoblasts are cells responsible for bone growth and for synthesizing bone matrix, whereas osteoclasts function in bone resorbtion. There are some osteogenic markers that osteoblasts are able to produce during bone formation, that have an important role in the processes of migration, proliferation and differentiation. Also, the differentiation process is governed by three major signaling pathways: Wnt, Hedgehog and Notch, which have the role to regulate osteogenesis by controlling proliferation, differentiation and apoptosis. This review aims to synthesize the most important information known so far regarding the main stages and markers of osteogenesis, as well as the main biomaterials, major cell types and specific osteogenic inducers involved in bone tissue engineering. Moreover, this review can serve as a guideline for further applications concerning this subject.

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Genes and Molecular Pathways of the Osteogenic Process

Cited by 14 publications

References 205 publications

Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

A comprehensive transcriptome of early development in yellowtail kingfish (Seriola lalandi)

An overview on osteogenic differentiation process: Minimum essential information for bone tissue engineering

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