Thermus Species 1995
DOI: 10.1007/978-1-4615-1831-0_7
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Genes and Genetic Manipulation in Thermus thermophilus

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Cited by 3 publications
(2 citation statements)
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“…Some of the current gaps in the technology are obvious: ‘plug-and-play’ laboratory expression hosts for the major groups of extremophiles (e.g., thermophilic bacteria and archaea, psychrophiles, acidophiles, halophiles), where intracellular expression is highly impacted by the extracellular environment (temperature, pH, salt), are only available for a limited range of organisms. Probably the first thermophilic organism to be genetically manipulated was Thermus thermophiles (Oshima 1995 ), and this organism has now been engineered as a thermophilic host for the over-expression of proteins that cannot be expressed in mesophilic systems (De Rose et al 2021a ). However, the very high G + C content of the DNA of this organism generally means that genes to be expressed in this host system have to be synthesized.…”
Section: Gene Discoverymentioning
confidence: 99%
“…Some of the current gaps in the technology are obvious: ‘plug-and-play’ laboratory expression hosts for the major groups of extremophiles (e.g., thermophilic bacteria and archaea, psychrophiles, acidophiles, halophiles), where intracellular expression is highly impacted by the extracellular environment (temperature, pH, salt), are only available for a limited range of organisms. Probably the first thermophilic organism to be genetically manipulated was Thermus thermophiles (Oshima 1995 ), and this organism has now been engineered as a thermophilic host for the over-expression of proteins that cannot be expressed in mesophilic systems (De Rose et al 2021a ). However, the very high G + C content of the DNA of this organism generally means that genes to be expressed in this host system have to be synthesized.…”
Section: Gene Discoverymentioning
confidence: 99%
“…We have, therefore, initiated a genetic dissection of ribosomes of the extreme thermophile Thermus thermophilus. Despite the promise of Thermus as a genetic system (16,21), the lack of selectable genetic markers such as transposons or antibiotic resistance mutations makes the mapping of mutations to ribosomal protein and RNA genes problematic. Here we report the isolation of a 23S rRNA mutation conferring resistance to the antibiotics erythromycin and lincomycin.…”
mentioning
confidence: 99%