“…Comparison of the EcoRI fragment patterns of the two independent plasmids from PS21 (Fig. Site-specificity with respect to both recombining molecules is seen in the integration of F and R factor plasmids into the bacterial chromosome; however, these events, although not dependent on RecA, occur at a much lower frequency in RecA-hosts [10]. 1, lane F) shows that a 16.2-kb fragment from pPS900 (arrow, lane B) and a 7-kb fragment from pPS030 (arrow, lane C) are missing from the cointegrate.…”
Enterotoxigenic Escherichia coli (ETEC) strains which cause diarrhea in young pigs often possess the proteinaceous surface antigen, K88. The genetic determinants for production of K88 fimbriae and utilization of raffinose (Raf) are located on non‐conjugative plasmids. We have examined some parameters of cointegrate formation between one of these plasmids, pPS900, and pPS030, a conjugative R factor. Cointegrate formation appears to be RecA‐independent and to involve specific regions of both plasmids. Cointegrates are unstable, breaking down to form plasmid species indistinguishable from pPS030 and pPS900. Stable cointegrates have undergone a deletion which often includes all or part of the region of pPS900 encoding K88 antigen production.
“…Comparison of the EcoRI fragment patterns of the two independent plasmids from PS21 (Fig. Site-specificity with respect to both recombining molecules is seen in the integration of F and R factor plasmids into the bacterial chromosome; however, these events, although not dependent on RecA, occur at a much lower frequency in RecA-hosts [10]. 1, lane F) shows that a 16.2-kb fragment from pPS900 (arrow, lane B) and a 7-kb fragment from pPS030 (arrow, lane C) are missing from the cointegrate.…”
Enterotoxigenic Escherichia coli (ETEC) strains which cause diarrhea in young pigs often possess the proteinaceous surface antigen, K88. The genetic determinants for production of K88 fimbriae and utilization of raffinose (Raf) are located on non‐conjugative plasmids. We have examined some parameters of cointegrate formation between one of these plasmids, pPS900, and pPS030, a conjugative R factor. Cointegrate formation appears to be RecA‐independent and to involve specific regions of both plasmids. Cointegrates are unstable, breaking down to form plasmid species indistinguishable from pPS030 and pPS900. Stable cointegrates have undergone a deletion which often includes all or part of the region of pPS900 encoding K88 antigen production.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.