Genes Affect the Timing of Early Mouse Embryo Development

Goldbard, Simon; Warner, Carol M.

doi:10.1095/biolreprod27.2.419

Cited by 70 publications

(28 citation statements)

References 14 publications

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“…In a recent study, Gao et al (2004) showed that hybrid B6D2F1 oocytes were able to support better in vitro development to the blastocyst stage after parthenogenetic activation with strontium than inbred C57BL/6 and DBA/2 oocytes. On the other hand, it is well documented that the rates of preimplantation embryo development both in vivo and in vitro are also strain dependent, and that embryos derived from hybrid strains develop more efficiently and faster than those derived from inbred and outbred strains (Goldbard & Warner 1982, Warner et al 1987, Du & Wales 1993, Scott & Whittingham 1996. Therefore, in light of these previous results by others and our results presented here, it is possible that strain-specific differences in the dynamics of oocyte activation could be related to later differences in the rate of early embryo development.…”

Section: Discussionmentioning

confidence: 99%

Effect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes

Ibáñez¹,

Albertini²,

Overström³

2005

Reproduction

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With the aim of investigating the effects of oocyte genotype and activating stimulus on the timing of nuclear events after activation, oocytes collected from hybrid B6D2F1, inbred C57BL/6 and outbred CF-1 and immunodeficient nude (NU/1 ) females were activated using ethanol or strontium and fixed at various time-points. Meiotic status, spindle rotation and second polar body (PB2) extrusion were monitored by fluorescence microscopy using DNA-, microtubule-and microfilament-selective probes. Although activation efficiency was similar in all groups of oocytes, a significant percentage of CF-1 and NU/1 oocytes treated with ethanol and of C57BL/6 oocytes treated either with ethanol or strontium failed to complete activation and became arrested at a new metaphase stage (MIII) after PB2 extrusion. C57BL/6 oocytes also showed slower release from MII arrest but faster progression to telophase (TII) after ethanol exposure, and they exhibited the most rapid exit from TII under both activation treatments. Strontium caused delayed meiotic resumption, spindle rotation and PB2 extrusion, but rapid TII exit, in B6D2F1, CF-1 and NU/1 oocytes when compared with ethanol. Compared with all other strains, NU/1 oocytes were significantly slower in completing spindle rotation and PB2 extrusion, irrespective of the activating stimulus, and a significant decrease in activation rates and pace of meiotic progression was observed after strontium exposure. Thus, our findings demonstrated that the kinetics of meiosis resumption and completion, spindle rotation and PB2 extrusion following parthenogenetic activation depends on both genotype-specific factors and on the activation treatment applied.

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Section: Discussionmentioning

confidence: 99%

Effect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes

Ibáñez¹,

Albertini²,

Overström³

2005

Reproduction

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“…The product of the Ped gene, the Qa-2 protein, is a mouse major histocompatibility complex (MHC) class Ib protein encoded by two almost identical genes (one nucleotide difference), Q7 and Q9 (6,8). However, other genes, in addition to the MHC encoded Ped, gene, play a role in the control of the rate of early preimplantation embryonic cleavage (9). Mouse strains (B6.K1 and B6.K2) that differ in only the Ped phenotype suggest that presence of Qa-2 protein (Ped fast) confers a reproductive advantage (10).…”

Section: Introductionmentioning

confidence: 99%

Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

Purnell

Warner

Kort

et al. 2006

J Assist Reprod Genet

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“…Interestingly, the cleavage rate of mouse embryos has been shown to be linked to a gene designated "Ped" (preimplantation embryo development gene; ref. 30), which has been linked to the MHC and correlated with the presence or absence of the Qa-2 antigen (31). The Ped phenotype is an intrinsic property of the embryo, independent of the uterine environment (32).…”

Section: Introductionmentioning

confidence: 99%

HLA-G expression during preimplantation human embryo development.

Jurisicova

Casper

MacLusky

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

232

143

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HLA-G is a nonclassical class I major histocompatibility complex molecule with a restricted pattern of expression that includes the placental extravillus cytotrophoblast cells in direct contact with maternal tissues. Circumstantial evidence suggests that HLA-G may play a role in protection of the semiallogeneic human fetus. We examined whether HLA-G is expressed during the critical period of preimplantation human development and whether expression of this molecule could be correlated with the cleavage rate of embryos. Using reverse transcription PCR on surplus human embryos and unfertilized oocytes from patients undergoing in vitro fertilization we detected HLA-G heavy chain mRNA in 40% of 148 of blastocysts tested. The presence of HLA-G mRNA was also detected in unfertilized oocytes and in early embryos, but not in control cumulus oophorus cells. beta 2-Microglobulin mRNA was also found in those embryos expressing HLA-G. In concordance with our mRNA data, a similar proportion of embryos stained positive for HLA-G utilizing a specific monoclonal antibody. Interestingly, expression of HLA-G mRNA was associated with an increased cleavage rate, as compared to embryos lacking HLA-G transcript. Thus, HLA-G could be a functional homologue of the mouse Qa-2 antigen, which has been implicated in differences in the rate of preimplantation embryo development. To our knowledge, the presence of HLA-G mRNA and protein in human preimplantation embryos and oocytes has not been reported previously. The correlation of HLA-G mRNA expression with cleavage rate suggests that this molecule may play an important role in human pre-embryo development.

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Genes Affect the Timing of Early Mouse Embryo Development

Abstract: The rate of preimplantation mouse embryo development was found to be influenced by genes both within and outside the H-2 complex.

Cited by 70 publications

References 14 publications

Effect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes

Effect of genetic background and activating stimulus on the timing of meiotic cell cycle progression in parthenogenetically activated mouse oocytes

Influence of the Preimplantation Embryo Development (Ped) Gene on Embryonic Platelet-Activating Factor (PAF) Levels

HLA-G expression during preimplantation human embryo development.

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