Generic phosphatase activity detection using zinc mediated aggregation modulation of polypeptide-modified gold nanoparticles

Abstract

A generic plasmonic assay for rapid detection and characterization of a wide range of phosphatases using different natural substrates is described that exploit the enzymatic release of inorganic phosphate to control the aggregation of nanoparticles functionalized with a designed Zn2+responsive polypeptide.

Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel

“…Gold nanoparticles (AuNPs) (20 nm, Cline Scientific AB) were functionalized with the peptide JR2EC as described previously 32 , 36 .…”

Pyridoxal derivative functionalized gold nanoparticles for colorimetric determination of zinc(ii) and aluminium(iii)

“…[23][24][25][26] Literature survey revealed that very few colorimetric methods for the detection of Al 3+ and Zn 2+ are reported using surface functionalized AuNPs, in compared to other metal ions such as Hg 2+ , Cu 2+ , Pb 2+ etc. [27][28][29][30][31][32][33][34][35] Also, the nanoprobes have been recently applied for the construction of Boolean logic gates, which are promising in the fabrication of molecular computation and becoming a potential substitute for the traditional silicon-based computation. [36][37][38][39][40][41] Continuing our research on the chemosensors development with vitamin B 6 cofactors like pyridoxal (PL), pyridoxal 5-phosphate (PLP), [42][43][44] herein, we have developed a rapid colorimetric nanoprobe with good selectivity for Al 3+ and Zn 2+ using an organic ligand functionalized AuNPs.…”

Zinc-Triggered Hierarchical Self-Assembly of Fibrous Helix–Loop–Helix Peptide Superstructures for Controlled Encapsulation and Release

“…JR2EC, JR2E and D-Ala-JR2EC (peptide identity and sequences in Supporting Information) were synthesized using Fmoc-strategy and sequentially purified as described 4 previously. 26 Linking two peptides via a disulfide bridge was carried out by dissolving peptides (2 mg/ml) in ammonium hydrogen carbonate buffer (10 mM, pH 8.5), and subsequent incubation for 72 hours at room temperature on an orbital-shaker. Formation of the disulfide bridge was confirmed by an Ellman's test of free thiols.…”

“…JR2EC functionalized AuNPs (40 nm in radius) were synthesized as described previously. 26 AuNPs ~70 nM (BBI Solutions, UK) were incubated with 10 µM JR2EC (10 mM sodium citrate, pH 6) at 4 °C overnight. After incubation the particles were repeatedly centrifuged (12,000 × g, 30 min) and the buffer subsequently exchanged with Bis-Tris buffer (30 mM, pH 7) until the concentration of unbound JR2EC was below 5 pM.…”

“…pH, [14][15][16] temperature, 17,18 metal ion coordination, [19][20][21] and by specific interactions with complementary peptides or other biomolecules. [22][23][24] The numerous possibilities to modulate the assembly process have enabled development of a wide range of peptidebased materials for applications in biosensing, 25,26 drug delivery, 27,28 and tissue engineering. 29,30 Metal cations are attractive modulators of peptide-directed self-assembly since peptides can coordinate certain cations with high specificity and affinity.…”