Generic approach to chiral separations: Chiral capillary electrophoresis with ternary cyclodextrin mixtures

Abstract

A generic approach to chiral separations using capillary electrophoresis is outlined. The generic method was developed by investigating the interactions of charged (sulfobutyl ether‐β‐cyclodextrin, SBE‐β‐CD) and neutral cyclodextrins (primarily γ‐ and dimethyl‐β‐cyclodextrin) in a low pH buffer (50 mM sodium dihydrogen phosphate, pH 2.5). Using a background electrolyte (BGE) containing 5% w/w SBE‐β‐CD, 25 mM γ‐cyclodextrin (γ‐CD) and 25 mM dimethyl‐β‐CD (DM‐β‐CD) dissolved in the phosphate buffer, resolution of more than 90% of the 26 randomly selected racemic pharmaceutical drugs or their synthetic intermediates was achieved. © 2001 John Wiley & Sons, Inc. J Micro Sep 12: 568–576, 2000