Generic amplification and next generation sequencing reveal Crimean-Congo hemorrhagic fever virus AP92-like strain and distinct tick phleboviruses in Anatolia, Turkey

Dinçer, Ender; Brinkmann, Annika; Hekimoğlu, Olcay; Hacıoğlu, Sabri; Földes, Katalin; Karapınar, Zeynep; Polat, Pelin Fatoş; Oğuz, Bekir; Kılınç, Özlem Orunç; Hagedorn, Peter; Özer, Nazmi; Nitsche, Andreas; Ergünay, Koray

doi:10.1186/s13071-017-2279-1

“…The variant of LTV described here was closely related to the variants found in Rhipicephalus microplus from China and Brazil, suggesting here again possible vector specificity [7,20]. However, although primarily described in Rhipicephalus microplus specimens, LTV has also been reported in Turkish Hyalomma marginatum and Rhipicephalus sanguineus ticks [53,56], and in Colombian Dermacentor nitens ticks (GenBank MK040531). In addition, we also detected LTV in Amblyomma variegatum specimens (10.4% to 11.9%).…”

Section: Discussionsupporting

confidence: 65%

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard¹,

Temmam²,

Devillers³

et al. 2019

Preprint

0

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Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

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“…The variant of LTV described here is closely related to the variants found in Rhipicephalus microplus from China and Brazil, again suggesting possible vector specificity [7,20]. However, although primarily described in Rhipicephalus microplus specimens, LTV has also been reported in Turkish Hyalomma marginatum and Rhipicephalus sanguineus ticks [53,56], and in Colombian Dermacentor nitens ticks (GenBank MK040531). In addition, we also detected LTV in Amblyomma variegatum specimens (10.4% to 11.9%).…”

Section: Discussionsupporting

confidence: 62%

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

Gondard

¹

,

Temmam

²

,

Devillers

³

et al. 2020

Viruses

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Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.

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“…Strikingly, all other sequences had an arginine at this position except for one other strain, MG48, which contained a lysine (K) ( Figure 2C ). The CCHFV-MG48 partial sequence also belongs to the Europe 2 lineage and was derived directly from a tick sample recently collected in the neighboring country of Turkey ( Dinçer et al, 2017 ). Importantly, this finding shows that variation at this position is detectable in ticks, whereas CCHFV isolated from mammalian cells or human samples does not contain this variation, possibly because variants with R at this position are preferentially amplified.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, studies of the changes in CCHFV genomic signatures upon transmission from ticks to humans or other susceptible species have been precluded. While Europe 2 CCHFV strains have often been detected directly in ticks collected in the Balkans and in Turkey ( Sherifi et al, 2014 ; Papa et al, 2014 ; Panayotova et al, 2016 ; Papa et al, 2017 ; Dinçer et al, 2017 ), no full-genome of a Europe 2 strain has been derived directly from a tick to date. Only two Europe 2 CCHFV full-genome sequences have been described, the AP92 and Pentalofos strains, both detected in Greece ( Deyde et al, 2006 ; Papa et al, 2018 ), but they were determined after passaging the virus in vertebrate culture systems.…”

Section: Introductionmentioning

confidence: 99%

A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells

Hua

¹

,

Scholte

²

,

Ohlendorf

³

et al. 2020

eLife

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Crimean-Congo Hemorrhagic Fever (CCHF) is the most widely distributed tick-borne viral infection in the world. Strikingly, reported mortality rates for CCHF are extremely variable, ranging from 5 to 80% (1). CCHF virus (CCHFV, Nairoviridae) exhibits extensive genomic sequence diversity across strains (2, 3). It is currently unknown if genomic diversity is a factor contributing to variation in its pathogenicity. We obtained complete genome sequences of CCHFV directly from the tick reservoir. These new strains belong to a solitary lineage named Europe 2 that is circumstantially reputed to be less pathogenic than the epidemic strains from Europe 1 lineage. We identified a single tick-specific amino acid variant in the viral glycoprotein region that dramatically reduces its fusion activity in human cells, providing evidence that a GPC variant, present in ticks, have severely impaired function in human cells.

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Generic amplification and next generation sequencing reveal Crimean-Congo hemorrhagic fever virus AP92-like strain and distinct tick phleboviruses in Anatolia, Turkey

Cited by 38 publications

References 62 publications

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

RNA Viruses of Amblyomma variegatum and Rhipicephalus microplus and Cattle Susceptibility in the French Antilles

A single mutation in Crimean-Congo hemorrhagic fever virus discovered in ticks impairs infectivity in human cells

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