geneRFinder: gene finding in distinct metagenomic data complexities

Abstract

MotivationMicrobes perform a fundamental economic, social and environmental role in our society. Metagenomics makes it possible to investigate microbes in their natural environments (the complex communities) and their interactions. The way they act is usually estimated by looking at the functions they play in those environments and their responsibility is measured by their genes. The advances of next-generation sequencing technology have facilitated metagenomics research however it also create a heavy computational burden. Large and complex biological datasets are available as never before. There are many gene predictors available which can aid gene annotation process though they lack of handling appropriately metagenomic data complexities. There is no standard metagenomic benchmark data for gene prediction. Thus, gene predictors may inflate their results by obfuscating low false discovery rates.ResultsWe introduce geneRFinder, a ML-based gene predictor able to outperform state-of-the-art gene prediction tools across this benchmark by using only one pre-trained Random Forest model. Average prediction rates of geneRFinder differed in percentage terms by 54% and 64%, respectively, against Prodigal and FragGeneScan while handling high complexity metagenomes. The specificity rate of geneRFinder had the largest distance against FragGeneScan, 79 percentage points, and 66 more than Prodigal. According to McNemar’s test, all percentual differences between predictors performances are statistically significant for all datasets with a 99% confidence interval.ConclusionsWe provide geneRFinder, a approach for gene prediction in distinct metagenomic complexities, available at github.com/railorena/geneRFinder, and also we provide a novel, comprehensive benchmark data for gene prediction — which is based on The Critical Assessment of Metagenome Interpretation (CAMI) challenge, and contains labeled data from gene regions – avaliable at sourceforge.net/p/generfinder-benchmark.