Generative and Recognising Devices for Biological Processes

Muhammad, M. Rashith; Kumar, Chandan; Selvakumar, R.

doi:10.1007/s13369-016-2030-9

Cited by 2 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in the diagnosis and therapy of cancer [7] as well as in in vitro DNAbased logic circuits and gates to diagnose the levels of miRNA [8]. Another approach focuses on the potential application of biocomputing machines to biological processes [9,10] and to monitor enzymatic regulation [11]. A technique called DNA manipulation has been used in the biomolecular implementation of digital circuits [12] as well as neural networks [13].…”

Section: Introductionmentioning

confidence: 99%

A detailed experimental study of a DNA computer with two endonucleases

Sakowski

Krasiński

Sarnik

et al. 2017

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Great advances in biotechnology have allowed the construction of a computer from DNA. One of the proposed solutions is a biomolecular finite automaton, a simple two-state DNA computer without memory, which was presented by Ehud Shapiro's group at the Weizmann Institute of Science. The main problem with this computer, in which biomolecules carry out logical operations, is its complexity - increasing the number of states of biomolecular automata. In this study, we constructed (in laboratory conditions) a six-state DNA computer that uses two endonucleases (e.g. AcuI and BbvI) and a ligase. We have presented a detailed experimental verification of its feasibility. We described the effect of the number of states, the length of input data, and the nondeterminism on the computing process. We also tested different automata (with three, four, and six states) running on various accepted input words of different lengths such as ab, aab, aaab, ababa, and of an unaccepted word ba. Moreover, this article presents the reaction optimization and the methods of eliminating certain biochemical problems occurring in the implementation of a biomolecular DNA automaton based on two endonucleases.

show abstract