Generations of sequencing technologies

Pettersson, Erik; Lundeberg, Joakim; Ahmadian, Afshin

doi:10.1016/j.ygeno.2008.10.003

Cited by 320 publications

(225 citation statements)

References 34 publications

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“…Other high-throughput techniques are based on different principles (e.g., reverse termination) and are discussed elsewhere (Pettersson et al 2009). Interestingly, a nonoptical genome sequencing has been developed (Rothberg et al 2011), which promises a better performance than traditional optical-based sequencing.…”

Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Gastrointestinal microorganisms in cats and dogs: a brief review

Garcia-Mazcorro¹,

Minamoto

2013

Arch. med. vet.

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RESUMENEl tracto gastrointestinal (GI) de animales contiene diferentes tipos de microorganismos conocido como la microbiota GI. Por mucho tiempo, la microbiota GI ha generado interés porque los microorganismos GI están involucrados en múltiples procesos fisiológicos en el hospedero, así perpetuando salud o enfermedad. Estudios recientes han demostrado que la microbiota GI de gatos y perros es tan compleja como en humanos y otros animales, revelado con el uso de tecnologías de secuencia modernas y otras técnicas moleculares. La microbiota GI incluye miembros de todos los tres dominios principales de vida (Archaea, Bacterias y Eucariotas), pero las bacterias son el grupo de microorganismos más abundante y metabólicamente activo. El estómago de gatos y perros esta principalmente poblado de Helicobacter spp., el cual en perros puede representar tanto como el 98% de toda la microbiota bacteriana en el estómago. El intestino delgado contiene una microbiota más diversa, conteniendo representantes de al menos cinco diferentes filos bacterianos (principalmente Firmicutes y Bacteroidetes). El intestino grueso contiene el grupo de bacterias más abundante (~10 11 células bacterianas por gramo de contenido intestinal), diverso (al menos diez diferentes filos han sido detectados) y metabólicamente relevante en el tracto GI. La mayoría de las bacterias en el intestino grueso son anaerobios estrictos, los cuales dependen de la fermentación de sustancias no digeridas para subsistir. Aunque estudios recientes han dilucidado las complejidades de la microbiota GI en gatos y perros, más investigación todavía es necesaria para encontrar maneras de manipular exitosamente los microorganismos GI para prevenir y/o tratar enfermedades GI.Palabras clave: gastrointestinal, microbiota, gatos, perros. SUMMARYThe gastrointestinal (GI) tract of animals contains different types of microorganisms known as the GI microbiota. The GI microbiota has long been of interest because of its involvement in multiple physiological processes in the host, influencing health or disease. Recent studies have shown that the GI microbiota of cats and dogs is as complex as the one present in humans and other animals, according to state-of-the-art sequencing technologies and other molecular techniques. The GI microbiota includes members of all three main life domains (Archaea, Bacteria, and Eukaryotes), with bacteria being the most abundant and metabolically active group of microorganisms. The stomach of cats and dogs is mainly inhabited by Helicobacter spp., which in dogs may account for as much as 98% of all gastric bacterial microbiota. The small intestine harbors a more diverse microbiota as it contains representatives from at least five bacterial phyla (mainly Firmicutes and Bacteroidetes). The large intestine harbors the most abundant (~10 11 bacterial cells per gram of intestinal content), diverse (at least 10 bacterial phyla have been identified) and physiologically relevant group of bacteria in the GI tract. Most bacteria in the large intestine are strict an...

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Section: Fluorescence In Situ Hybridization (Fish)mentioning

confidence: 99%

Gastrointestinal microorganisms in cats and dogs: a brief review

Garcia-Mazcorro¹,

Minamoto

2013

Arch. med. vet.

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“…In an attempt to bring some clarity to classification we have divided DNA sequencing technologies into three generations (Pettersson et al 2009). …”

Section: Classification Of Technologiesmentioning

confidence: 99%

“…In an attempt to address this short-coming, a diverse array of new sequencing technologies have been developed and are currently in development. Although the widely perceived aim of practical and affordable whole genome sequencing is ambitious, requiring major improvements in run capacity, speed of processing and cost, progress to date has been remarkable (see reviews Mardis 2011;Metzker 2010;Pettersson et al 2009;Tucker et al 2009;Voelkerding et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Review of massively parallel DNA sequencing technologies

Moorthie

Mattocks

Wright

2011

HUGO J

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Since the development of technologies that can determine the base-pair sequence of DNA, the ability to sequence genes has contributed much to science and medicine. However, it has remained a relatively costly and laborious process, hindering its use as a routine biomedical tool. Recent times are seeing rapid developments in this field, both in the availability of novel sequencing platforms, as well as supporting technologies involved in processes such as targeting and data analysis. This is leading to significant reductions in the cost of sequencing a human genome and the potential for its use as a routine biomedical tool. This review is a snapshot of this rapidly moving field examining the current state of the art, forthcoming developments and some of the issues still to be resolved prior to the use of new sequencing technologies in routine clinical diagnosis.

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“…This difference enables a highly miniaturized and parallelized procedure and technique (see figure 3). For more details see Ronaghi, 2001;Shendure et al, 2004;Ellegren, 2008;Hudson, 2008;Petterson et al, 2009;Voelkerding et al, 2009. Fig.…”

Section: The Sequencing Process -A Typical Case For Outsourcingmentioning

confidence: 99%