Generation(s) in The Waves and The Years

Hanson, Clare

doi:10.1007/978-1-349-23381-6_5

Cited by 1 publication

(2 citation statements)

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“…Recently, a number of studies in cultured cells have also proposed novel functions for G s ␣ which are independent of PKA (48)(49)(50)(51). In each case, however, further study has shown that the effects observed are likely to be indirect and depend instead on the activation of PKA (46,47). At this time then, the only effectors whose activity is clearly modulated directly by G s ␣ are AC isoforms.…”

Section: Discussionmentioning

confidence: 99%

“…Phenotypes arising from activation of DG s ␣ pathways should critically depend on activation of PKA, given the traditional scheme developed in cultured mammalian cells. Consistent with this prediction, many studies have demonstrated that response of cultured cells to activation of this pathway through expression of G s ␣* depends on PKA (15,46,47). To test whether this pathway is acting in wing epithelial cells to generate blistering, we initially employed a dominant-negative form of the regulatory subunit of PKA (R dn ) to inhibit PKA activity in these cells (33,56).…”

Section: Gal4 Mediated Expression Of Dg S ␣*mentioning

confidence: 91%

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Activation of protein kinase A-independent pathways by G _s α in Drosophila

Wolfgang

Roberts

Quan

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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One of the best-described transmembrane signal transduction mechanisms is based on receptor activation of the ␣ subunit of the heterotrimeric G protein G s , leading to stimulation of adenylyl cyclase and the production of cAMP. Intracellular cAMP is then thought to mediate its effects largely, if not entirely, by activation of protein kinase A and the subsequent phosphorylation of substrates which in turn control diverse cellular phenomena. In this report we demonstrate, by two different methods, that reduction or elimination of protein kinase A activity had no effect on phenotypes generated by activation of G s ␣ pathways in Drosophila wing epithelial cells. These genetic studies show that the G s ␣ pathway mediates its primary effects by a novel pathway in differentiating wing epithelial cells. This novel pathway may in part be responsible for some of the complex, cell-specific responses observed following activation of this pathway in different cell types.

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