2012
DOI: 10.1128/jvi.06157-11
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Generation of Transmitted/Founder HIV-1 Infectious Molecular Clones and Characterization of Their Replication Capacity in CD4 T Lymphocytes and Monocyte-Derived Macrophages

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Cited by 309 publications
(390 citation statements)
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References 71 publications
(96 reference statements)
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“…pNL43-ADA(Env)-GFP.IRES.Nef proviral vectors (deleted for vpu, nef, and nef and vpu or expressing the D368R Env variant) and the VSVG-encoding plasmid (pSVCMV-IN-VSV-G) were previously described (12,51). T/F and corresponding 6-mo consensus IMCs of patients CH58 and CH77 were inferred, constructed, and biologically characterized as described (36)(37)(38)(39).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…pNL43-ADA(Env)-GFP.IRES.Nef proviral vectors (deleted for vpu, nef, and nef and vpu or expressing the D368R Env variant) and the VSVG-encoding plasmid (pSVCMV-IN-VSV-G) were previously described (12,51). T/F and corresponding 6-mo consensus IMCs of patients CH58 and CH77 were inferred, constructed, and biologically characterized as described (36)(37)(38)(39).…”
Section: Methodsmentioning
confidence: 99%
“…To ensure that sensitization of HIV-1-infected cells by CD4 mimetics was also observed when using full-length clinically relevant primary HIV-1 isolates, we infected primary CD4 T cells with extensively characterized infectious molecular clones (IMCs) constructed from two transmitted/founder (T/F) and their corresponding 6-mo consensus sequences (36)(37)(38)(39). Primary viruses are known to exhibit low Env reactivity and, as such, have little or no intrinsic exposure of CD4i epitopes (40).…”
Section: Cd4 Mimetics Enhance Recognition and Killing Of Cells Infectmentioning
confidence: 99%
“…Viral plasmids (courtesy of Dr John Kappes [24]) for two T/F viruses, CH040 and CH077 (both subtype B), were derived from single genome amplification of viral RNA from acutely infected individuals. Two laboratory-adapted strains, NL4·3 and YU2, came from the UAB Center for AIDS Research (courtesy of Dr John Kappes).…”
Section: Hiv-1 Virus Stocksmentioning
confidence: 99%
“…in activated CD4 + T cells but not macrophages (14,(22)(23)(24)(25). Moreover, analysis of a comprehensive panel of infectious molecular clones (IMCs) showed that TF viruses packaged more envelope glycoprotein (Env), exhibited greater infectivity, bound to monocyte-derived dendritic cells more efficiently, and replicated to higher titers in CD4 + T cells in the presence of the type 1 interferon IFNα2 than chronic control (CC) viruses (26).…”
mentioning
confidence: 99%