Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA

Hirabayashi, Masumi; Hochi, Shinichi

doi:10.1007/978-1-60327-389-3_9

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Parrington et al more efficient when the sperm were pretreated with Triton-X or subjected to repeated freeze-thaw cycles prior to being incubated with exogenous DNA; such treatments are thought to cause disruption of the sperm membrane which could facilitate uptake and integration of exogenous DNA [Szczygiel et al 2003]. Recently the efficiency of production of both transgenic mice and rats by SMGT/ ICSI was shown to be improved when membrane structure of sperm heads was partially disrupted by detergent or ultrasonic treatment before exposure to the exogenous DNA solution [Hirabayashi and Hochi 2010]. In pigs, the integrity of the sperm plasma membrane has been shown to play a critical role in DNA interaction, and altered plasma membranes facilitate interactions between an injected exogenous DNA and the sperm chromatin.…”

Section: Sperm Mediated Gene Transfermentioning

confidence: 99%

Sperm and testis mediated DNA transfer as a means of gene therapy

Parrington

Coward

Gadea

2011

Systems Biology in Reproductive Medicine

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Assisted reproductive technologies (ART) have revolutionized the treatment of infertility. However, many types of infertility may still not be addressable by ART. With recent successes in identifying many of the genetic factors responsible for male infertility and the future prospect of whole individual human genome sequencing to identify disease causing genes, the possible use of gene therapy for treating infertility deserves serious consideration. Gene therapy in the sperm and testis offers both opportunities and obstacles. The opportunities stem from the fact that numerous different approaches have been developed for introducing transgenes into the sperm and testis, mainly because of the interest in using sperm mediated gene transfer and testis mediated gene transfer as ways to generate transgenic animals. The obstacles arise from the fact that it may be very difficult to carry out gene therapy of the testis and sperm without also affecting the germline. Here we consider new developments in both sperm and testis mediated gene transfer, including the use of viral vectors, as well as the technical and ethical challenges facing those who would seek to use these approaches for gene therapy as a way to treat male infertility.

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Section: Sperm Mediated Gene Transfermentioning

confidence: 99%

Sperm and testis mediated DNA transfer as a means of gene therapy

Parrington

Coward

Gadea

2011

Systems Biology in Reproductive Medicine

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“…In addition to studying the fundamental aspects around fertilization or to overcome male factor infertility, the homologous ICSI technique is used for offspring production with spermatozoa freeze‐dried and rehydrated (Wakayama & Yanagimachi ), retrieved from testicular tissues (Silber et al ) or exposed to foreign DNA (Perry et al ; Hirabayashi & Hochi ). However, the ICSI procedure may disturb some post‐fertilization events essential for early development, including sperm‐induced oocyte activation, epigenetic remodeling of paternal genomes, and microtubule organization for pronuclear fusion (Yoshizawa et al ; Hara et al ).…”

Section: Chemical Treatment In Bovine Icsimentioning

confidence: 99%

Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies

Hochi

2016

Animal Science Journal

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Centrosome integrity and microtubule network are crucial to the events around fertilization, including pronuclear development, migration and fusion, and the first mitotic division. The present review highlights the importance of bull spermatozoal centrosomes to function as a microtubule‐organizing center for successful fertilization and the subsequent embryonic development. Spermatozoal centrosomes need to be blended with ooplasmic pericentriolar materials accurately to nucleate and organize the sperm aster. Dysfunction of the spermatozoal centrosomes is associated with fertilization failure, which has been overcome with supplemental stimuli for oocyte activation following intracytoplasmic sperm injection in humans. Even though the spermatozoal centrosomes are functionally intact, abnormal sperm aster formation was frequently observed in vitrified‐warmed bovine oocytes, with delayed pronuclear development and migration. Treatment of the post‐warm oocytes with Rho‐associated coiled‐coil kinase inhibitor or α‐tocopherol inhibited the incidence of the abnormal aster formation, resulting in higher blastocyst yields following in vitro fertilization and culture. Thus, understanding of centrosomal function made it possible to improve the performance of advanced reproductive technologies.

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“…Gordon et al [2] first described the introduction of a foreign gene into mice using pronuclear injection into oocytes, an approach which had since been widely employed to study the molecular and cellular functions of many genes. Since then, transgenic methods, such as microinjection [3], sperm vector [4,5], ICSI-mediated transgenesis [6][7][8], transfection-mediated transgenesis of male germ stem cell in vitro [9] and in vivo [10,11], as well as female germ stem cell [12,13], transgenic by somatic cell nuclear transfer (SCNT) [14][15][16], lentiviral vectors [17][18][19] and primordial germ cells (PGCs) [20,21], have been used successfully to produce transgenic mice and other transgenic animal species such as rats, fish, pigs, sheep, cattle, rabbits, dogs, chickens, and monkey. As new technologies and techniques are developing, transgenic animal technologies will also be improved.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in the development of new transgenic animal technology

Miao

2012

Cell. Mol. Life Sci.

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Transgenic animal technology is one of the fastest growing biotechnology areas. It is used to integrate exogenous genes into the animal genome by genetic engineering technology so that these genes can be inherited and expressed by offspring. The transgenic efficiency and precise control of gene expression are the key limiting factors in the production of transgenic animals. A variety of transgenic technologies are available. Each has its own advantages and disadvantages and needs further study because of unresolved technical and safety issues. Further studies will allow transgenic technology to explore gene function, animal genetic improvement, bioreactors, animal disease models, and organ transplantation. This article reviews the recently developed animal transgenic technologies, including the germ line stem cell-mediated method to improve efficiency, gene targeting to improve accuracy, RNA interference-mediated gene silencing technology, zinc-finger nuclease gene targeting technology and induced pluripotent stem cell technology. These new transgenic techniques can provide a better platform to develop transgenic animals for breeding new animal varieties and promote the development of medical sciences, livestock production, and other fields.

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Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA

Cited by 4 publications

References 15 publications

Sperm and testis mediated DNA transfer as a means of gene therapy

Sperm and testis mediated DNA transfer as a means of gene therapy

Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies

Recent advances in the development of new transgenic animal technology

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