Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo

Sehgal, Lalit; Usmani, Abul; Dalal, Sorab N.; Majumdar, Subeer S.

doi:10.1007/978-1-4939-1215-5_18

Cited by 7 publications

(6 citation statements)

References 21 publications

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“…Methodologies for producing transgenic animals through the use of SSCs have been pursued for many years, and recent results indicate that this technique is not only feasible but may have unique advantages over other commonly used approaches. 6 …”

Section: Introductionmentioning

confidence: 99%

Plasticity of spermatogonial stem cells

Cooke¹,

Simon²,

Nanjappa³

et al. 2015

Asian J Androl

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There have been significant breakthroughs over the past decade in the development and use of pluripotent stem cells as a potential source of cells for applications in regenerative medicine. It is likely that this methodology will begin to play an important role in human clinical medicine in the years to come. This review describes the plasticity of one type of pluripotent cell, spermatogonial stem cells (SSCs), and their potential therapeutic applications in regenerative medicine and male infertility. Normally, SSCs give rise to sperm when in the testis. However, both human and murine SSCs can give rise to cells with embryonic stem (ES) cell-like characteristics that can be directed to differentiate into tissues of all three embryonic germ layers when placed in an appropriate inductive microenvironment, which is in contrast to other postnatal stem cells. Previous studies have reported that SSCs expressed an intermediate pluripotent phenotype before differentiating into a specific cell type and that extended culture was necessary for this to occur. However, recent studies from our group using a tissue recombination model demonstrated that SSCs differentiated rapidly into another tissue, in this case, prostatic epithelium, without expression of pluripotent ES cell markers before differentiation. These results suggest that SSCs are capable of directly differentiating into other cell types without going through an intermediate ES cell-like stage. Because SSCs do not require reprogramming to achieve a pluripotent state, they are an attractive source of pluripotent cells for use in regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Plasticity of spermatogonial stem cells

Cooke¹,

Simon²,

Nanjappa³

et al. 2015

Asian J Androl

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show abstract

“…The increasing facility of gene editing via nucleotide-directed endonucleases such as the CRISPR/Cas9 system may provide an opportunity to investigate hibernation phenotypes in Zapus using reverse genetics. Such an approach -for example, knocking out a gene of interest to observe the effect on a hibernation phenotype -would require a concerted effort to understand Zapus reproductive physiology and its compatibility with standard methods of gene editing that require manipulation of pre-implantation embryos, or the use of non-standard but potentially more feasible approaches to creating germline gene edits, such as microinjection and in vivo electroporation of adult testes or late-stage embryos with gene editing constructs [79,80]. Once animals harboring a modified allele are available, the breeding strategies needed to generate study cohorts of appropriate genotypes are expected to be practicable.…”

Section: Discussionmentioning

confidence: 99%

Breeding and hibernation of captive meadow jumping mice (Zapus hudsonius)

Brem

McNulty

Israelsen

2020

Preprint

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Hibernating mammals exhibit unique metabolic and physiological phenotypes that have potential applications in medicine or spaceflight, yet our understanding of the genetic basis and molecular mechanisms of hibernation is limited. The meadow jumping mouse, a small North American hibernator, exhibits traits – including a short generation time – that would facilitate genetic approaches to hibernation research. Here we report the collection, captive breeding, and laboratory hibernation of meadow jumping mice. Captive breeders in our colony produced a statistically significant excess of male offspring and a large number of all-male and all-female litters. We confirmed that short photoperiod induced pre-hibernation fattening, and cold ambient temperature facilitated entry into hibernation. During pre-hibernation fattening, food consumption exhibited non-linear dependence on both body mass and temperature, such that food consumption was greatest in the heaviest animals at the coldest temperatures. Meadow jumping mice exhibited a strong circadian rhythm of nightly activity that was disrupted during the hibernation interval. We quantified the length and timing of torpor bouts and arousals obtained from an uninterrupted recording of a hibernating female. Over a 90.6 day hibernation interval, torpor bouts ranged from 2.1 to 12.8 days (mean 7.7 days), and arousal length was relatively constant with a mean length of 9.6 hours. We conclude that it is possible to study hibernation phenotypes using captive-bred meadow jumping mice in a laboratory setting.

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“…The increasing facility of gene editing via nucleotide-directed endonucleases such as the CRISPR/Cas9 system may provide an opportunity to investigate hibernation phenotypes in Zapus using reverse genetics. Such an approach-for example, knocking out a gene of interest to observe the effect on a hibernation phenotype-would require a concerted effort to understand Zapus reproductive physiology and its compatibility with standard methods of gene editing that require manipulation of pre-implantation embryos, or the use of non-standard but potentially more feasible approaches to creating germline gene edits, such as microinjection and in vivo electroporation of adult testes or late-stage embryos with gene editing constructs [77,78]. Once animals harboring a modified allele are available, the breeding strategies needed to generate study cohorts of appropriate genotypes are expected to be practicable.…”

Section: Plos Onementioning

confidence: 99%

Breeding and hibernation of captive meadow jumping mice (Zapus hudsonius)

2021

View full text Add to dashboard Cite

Hibernating mammals exhibit unique metabolic and physiological phenotypes that have potential applications in medicine or spaceflight, yet our understanding of the genetic basis and molecular mechanisms of hibernation is limited. The meadow jumping mouse, a small North American hibernator, exhibits traits–including a short generation time–that would facilitate genetic approaches to hibernation research. Here we report the collection, captive breeding, and laboratory hibernation of meadow jumping mice. Captive breeders in our colony produced a statistically significant excess of male offspring and a large number of all-male and all-female litters. We confirmed that short photoperiod induced pre-hibernation fattening, and cold ambient temperature facilitated entry into hibernation. During pre-hibernation fattening, food consumption exhibited non-linear dependence on both body mass and temperature, such that food consumption was greatest in the heaviest animals at the coldest temperatures. Meadow jumping mice exhibited a strong circadian rhythm of nightly activity that was disrupted during the hibernation interval. We conclude that it is possible to study hibernation phenotypes using captive-bred meadow jumping mice in a laboratory setting.

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Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo

Cited by 7 publications

References 21 publications

Plasticity of spermatogonial stem cells

Plasticity of spermatogonial stem cells

Breeding and hibernation of captive meadow jumping mice (Zapus hudsonius)

Breeding and hibernation of captive meadow jumping mice (Zapus hudsonius)

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