Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp ( ) as a response to β-glucan and sulphated polysaccharide

Campa‐Córdova, Ángel I.; Hernández-Saavedra, Norma Y.; Philippis, Roberto De; Ascencio, Felipe

doi:10.1006/fsim.2001.0377

Cited by 226 publications

(107 citation statements)

References 40 publications

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“…The pro-inflammatory effect elicited by Cf-PLS is in line with literature that demonstrated an immunostimulant activity of the species of juvenile prawn Litopenaeus vannamei after immersion in a solution rich in sulfated-polysaccharide from the microalgae Cyanotheca sp. 23) Besides, carrageenan, a sulfated polysaccharide from marine red algae, is classically used as a positive control in experimental models of inflammation because its pro-inflammatory events, that involve cell migration and participation of a diversity of mediators. 24) In addition, it was demonstrated that a sulfated-polysaccharide fraction from the brown algae Porphyra haitanesis shows in vivo antioxidant property and increases the spleen and thymus size.…”

Section: Resultsmentioning

confidence: 99%

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Assreuy

Gomes

Silva

et al. 2008

Biological & Pharmaceutical Bulletin

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Sulfated-polysaccharides are complex macromolecular constituents of the extracellular matrix of marine algae that play an important role in mechanical, osmotic and ionic regulation.1) In Biomedicine the anticoagulant and antithrombotic properties have been most widely exploited and, at least for galactans, appear to be dependent on the sulfatation content and/or position of the sulfate groups.2) A natural sulfated polymer of L-fucose (fucoidan) showed a dose-dependent inhibition of leukocyte migration in the in vivo peritonitis model, 3) and sulfated fucans isolated from brown algae showed potent in vitro and in vivo inhibition of the human complement system. 4,5) It was demonstrated that a sulfatedpolysaccharide fraction extracted from the brown algae Porphyra haitanesis presents an in vivo antioxidant property, causing an increase in the spleen and thymus size, suggestive of an immunostimulant action. 6) Additionally, sulfated galactans of the red marine algae Bryothamnion seaforthii presented antinociceptive activity in mice 7) and of the red micro algae Pophyridium sp. anti-inflammatory action inhibiting eritema formation after topical use in humans.8) However, studies of sulfated galactans role in models of inflammation in vivo are rare in the literature. Here we isolated and investigated the effects of a sulfated galactan from the red marine algae Champia feldmannii, widely encountered along the South East and North East Brazilian sea coast, in experimental models of acute inflammation, coagulation and nociception. MATERIALS AND METHODS AnimalsMale Wistar rats (150-250 g) and Swiss mice (25-35 g) were maintained with a controlled 12/12 h light/dark cycle, at a temperature of 25°C with free access to food and water. The experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee of the State University of Ceará (UECE), Fortaleza-CE, Brazil, in accordance with international guidelines (NIH publication No. 85-23, revised 1985).Algae Champia feldmannii belongs to the order Rhodymeniales, family Lomentariaceae and was collected in the Pacheco beach of Caucaia, Ceará, Brazil. Algae were classified by Wladimir R. L. Farias, Department of Fishery Engeneering of Federal University of Ceará-Brazil.Erythrocytes Human erythrocytes were obtained from healthy donors at the Hematology Center of the Federal University of Ceará-Brazil.Drugs and Reagents Dextran sulphate, L-carrageenan, Evans blue, zymosan, morphine, cethylpiridinium chloride, sodium acetate, calcium chloride, ketamine, sulphuric acid, papain, 1-9-di-metylene blue (DMB), ethylenodiaminotetracetic acid (EDTA), indomethacin, dexamethasone, pentoxifylline, L-N-nitro-arginine methyl ester (L-NAME), meclyzine and formamide, N-acetyl-N,N,N-trimethylammonium bromide, 1,3-diaminopropane, toluidine blue, agarose gel (Sigma Chemical Co., St. Louis, MO, U.S.A. or SigmaAldrich Chemie, Steinheim, Germany); absolute ethanol, sodium chloride, sodium dodecyl sulfate (SDS) and 2-mer- 907, 60.455-970, Fortaleza-Ceará-Brasil. R...

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Section: Resultsmentioning

confidence: 99%

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Assreuy

Gomes

Silva

et al. 2008

Biological & Pharmaceutical Bulletin

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“…Song and Hsieh [13] first demonstrated a respiratory burst in Penaeus monodon haemocytes. It has been reported in haemocytes of Penaeus stylirostris [14], Penaeus vannamei [15], Macrobrachium rosenbergii [16] and Litopenaeus vannamei [17].…”

Section: Introductionmentioning

confidence: 94%

Effect of replacement of fish meal by meat and bone meal and poultry by-product meal in diets on the growth and immune response of Macrobrachium nipponense

Yang

Xie

et al. 2004

Fish & Shellfish Immunology

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“…Numerous studies have implicated SOD2 in the immune response, but few have defined functional roles for SOD2 in immunity. SOD2 is upregulated in response to lipopolysaccharide (LPS), poly(I·C), beta-glucan, and numerous pathogens in multiple cell types and organisms (12)(13)(14)(15)(16)(17). Functionally, SOD2 was found to be necessary for the phorbol myristate acetate-induced respiratory burst response and cell survival upon poly(I·C) exposure in vitro (16,17).…”

mentioning

confidence: 99%

Neutralization of Mitochondrial Superoxide by Superoxide Dismutase 2 Promotes Bacterial Clearance and Regulates Phagocyte Numbers in Zebrafish

et al. 2015

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T he roles that mitochondria play in antiviral signaling, via mitochondrial antiviral-signaling protein and promotion of inflammation and apoptosis, are well established (1-3); however, their importance in innate immunity is only now becoming clear. While mitochondria can promote inflammation via NF-B signaling and NLRP3 inflammasome formation, inflammation can lead to mitochondrial dysfunction, which can compound the severity of exaggerated inflammatory conditions such as sepsis (4-10). As the site of the electron transport chain within cells, mitochondria are a major source of reactive oxygen species (ROS) (mainly superoxide anions). ROS play diverse roles in cellular and organismal health, especially in innate immunity and inflammation. While the use of ROS to clear infections is beneficial to the host, inappropriate ROS production or lack of ROS neutralization can damage host DNA, proteins, and cell membranes. ROS-induced cellular damage can contribute to the undesired side effects of infectious and inflammatory diseases. Mechanisms are in place in hosts, and even some pathogens, to chemically convert ROS into less toxic compounds; however, overproduction of ROS can overwhelm host antioxidants. Thus, a better understanding of the impact of mitochondria, ROS, and mechanisms for neutralization of ROS on innate immunity could lead to improved treatments for infectious diseases and inflammatory disorders.The cellular mechanisms to neutralize ROS include the glutathione system, catalases, and the superoxide dismutase (SOD) family of enzymes. As superoxide producers, mitochondria are equipped with nuclear-encoded, mitochondrially localized SODs (SOD2, MnSOD) that convert superoxide into hydrogen peroxide. The deleterious effects of mitochondrial superoxide are demonstrated by mutations in SOD2 being implicated in idiopathic cardiomyopathy, age-related macular degeneration, aberrant brain morphology, motor neuron disease, vascular complications of diabetes, and cancer, whereas overexpression of SOD2 increases the Drosophila life span (11,(69)(70)(71)(72). Despite the importance of SOD2 and the regulation of ROS for health, surprisingly little is known about the role of SOD2 in immunity. Numerous studies have implicated SOD2 in the immune response, but few have defined functional roles for SOD2 in immunity. SOD2 is upregulated in response to lipopolysaccharide (LPS), poly(I·C), beta-glucan, and numerous pathogens in multiple cell types and organisms (12-17). Functionally, SOD2 was found to be necessary for the phorbol myristate acetate-induced respiratory burst response and cell survival upon poly(I·C) exposure in vitro (16,17). In a mouse model with SOD2 deleted specifically from thymocytes, the animals did not mount an effective adaptive immune response to influenza virus infection because of disrupted T-cell

show abstract

Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp ( ) as a response to β-glucan and sulphated polysaccharide

Cited by 226 publications

References 40 publications

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Biological Effects of a Sulfated-Polysaccharide Isolated from the Marine Red Algae Champia feldmannii

Effect of replacement of fish meal by meat and bone meal and poultry by-product meal in diets on the growth and immune response of Macrobrachium nipponense

Neutralization of Mitochondrial Superoxide by Superoxide Dismutase 2 Promotes Bacterial Clearance and Regulates Phagocyte Numbers in Zebrafish

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