Generation of superoxide and in vitro inactivation of viruses by aldopentoses.

Kashimura, Naoki; Sato, Ikuo; Kumazawa, Zenzaburo; Kuno, Hirokazu; Koyama, Shiro; Kitagawa, Masaru

doi:10.1271/bbb1961.46.2407

Cited by 5 publications

(3 citation statements)

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“…S-9 was treated with the various procedures described in this table. After these treatments, the reducing activities on the mutagenicities of some mutagens were determined as described in the text and Table I 9 7 38…”

Section: Some Properties Of the Mutagenicity-reducingmentioning

confidence: 99%

Mutagenicity of and Formation of Oxygen Radicals by Trioses and Glyoxal Derivatives

Yamaguchi

Nakagawa

1983

Agricultural and Biological Chemistry

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Section: Some Properties Of the Mutagenicity-reducingmentioning

confidence: 99%

Mutagenicity of and Formation of Oxygen Radicals by Trioses and Glyoxal Derivatives

Yamaguchi

Nakagawa

1983

Agricultural and Biological Chemistry

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“…The reduction of Nitro Blue Tetrazolium to formazan by aldopentoses was monitored spectrophotometrically in an adaption of the method of Kashimura et al (1982). The reaction mixture consisted of 0.15 mM-Nitro Blue Tetrazolium and 0.1 mM-EDTA (dipotassium salt) in 3 ml of 0.05 M-sodium bicarbonate buffer, pH 10.2, and an aldopentose, generally D(+)-xylose (10 mg/ml).…”

Section: Evaluation Of Aldose Reductase Inhibitors Against Generated mentioning

confidence: 99%

Purified rat lens aldose reductase. Polyol production in vitro and its inhibition by aldose reductase inhibitors

Kador¹,

Kinoshita²,

Brittain³

et al. 1986

Biochemical Journal

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The production of polyols in vitro by highly purified aldose reductase (EC 1.1.1.21) was monitored by g.l.c. In the presence of NADPH aldose reductase reduced glucose, galactose and xylose to the respective polyols sorbitol, galactitol and xylitol. The rates of formation of these polyols closely mirrored the Km values for the substrates obtained from kinetic measurements that monitored the rate of disappearance of NADPH. No polyol production occurred in the absence of purified aldose of purified aldose reductase, and analysis by g.l.c. revealed only the presence of unchanged monosaccharides. Addition of the aldose reductase inhibitor sorbinil to purified rat lens aldose reductase incubated with xylose in the presence of NADPH resulted in decreased xylitol production. However, aldose reductase inhibitors produced no effect in altering the rate of Nitro Blue Tetrazolium formation from either glucose or xylose, indicating that the observed inhibition in vitro does not result from a free-radical-scavenger effect.

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“…Oxygen radicals generated by the autoxidation of the reducing agents are mainly responsible for the observed biological activity.I,2) Common reducing aldoses and ketoses do not usually show virusinactivating ability. 3) Recently however, we have reported 4 ,S) that some reducing sugar phosphates generate superoxide at pH 10.4 and that they exhibit phagocidal action similar to that caused by quinone antibiotics and reductones. Moreover, we have examined the virus-inactivating ability of some other moderately autoxidizable sugar derivatives, such as oxidized polysaccharides 6 ) and pyranosiduloses.…”

mentioning

confidence: 98%