Generation of self-compatible diploid potato by knockout of S-RNase

Ye, Mingwang; Peng, Zhen; Tang, Dié; Yang, Zhuo; Li, Dawei; Yang, Xu; Zhang, Chengqi; Huang, Sanwen

doi:10.1038/s41477-018-0218-6

Cited by 171 publications

(129 citation statements)

References 22 publications

Supporting

Mentioning

117

Contrasting

Order By: Relevance

“…Both studies were highly successful in terms of the specific traits that they targeted, doubling the yield of S pimpinellifolium and increasing lycopene contents to 500% of control levels (Li et al, 2018b;Zs€ og€ on et al, 2018). A further example of the power of gene editing in breeding is provided by the recent demonstration that knocking out the self-incompatibility gene S-RNase allows redomestication of potato into an inbred-line based diploid crop, representing a promising alternative to traditional clonal propagation of tetraploid potato (Ye et al, 2018). The study demonstrated the utility of this approach in four different Solanum tuberosum clones, thus opening up the myriad possibilities in future diploid breeding, which will likely greatly facilitate both basic research and genetic improvement of potato and self-incompatible crops.…”

Section: De Novo Domesticationmentioning

confidence: 99%

De Novo Domestication: An Alternative Route toward New Crops for the Future

2019

View full text Add to dashboard Cite

Current global agricultural production must feed over 7 billion people. However, productivity varies greatly across the globe and is under threat from both increased competitions for land and climate change and associated environmental deterioration. Moreover, the increase in human population size and dietary changes are putting an ever greater burden on agriculture. The majority of this burden is met by the cultivation of a very small number of species, largely in locations that differ from their origin of domestication. Recent technological advances have raised the possibility of de novo domestication of wild plants as a viable solution for designing ideal crops while maintaining food security and a more sustainable lowinput agriculture. Here we discuss how the discovery of multiple key domestication genes alongside the development of technologies for accurate manipulation of several target genes simultaneously renders de novo domestication a route toward crops for the future.

show abstract

Section: De Novo Domesticationmentioning

confidence: 99%

De Novo Domestication: An Alternative Route toward New Crops for the Future

2019

View full text Add to dashboard Cite

show abstract

“…SC was retained in these trees over several years without obvious adverse effects. Much more recently, CRISPR-induced knockouts of S-RNase have been used to generate SC diploid lines for breeding purposes in potato (Solanum tuberosum L.) (Ye et al, 2018;Enciso-Rodriguez et al, 2019) (Figure 1B).…”

Section: Different Sources Of Sc: a Favorable Trait For Yield Enhancementioning

confidence: 99%

“…However, the introgression of the S. chacoense Sli gene into other potatoes is time-consuming and may carry along undesirable traits such as long stolons or high tuber glycoalkaloid content. CRISPR knock-out of the S-RNase gene has recently been shown to be another viable method to generate SC potato lines avoiding linkage drag of undesirable traits associated with Sli (Ye et al, 2018;Enciso-Rodriguez et al, 2019). This important milestone has implications for the replacement of the current tetraploid asexually propagated potato with a diploid inbred line-based crop propagated by seeds (Jansky et al, 2016).…”

Section: Ways Of Achieving Sc and Its Role In Hybrid Breedingmentioning

confidence: 99%

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

et al. 2020

View full text Add to dashboard Cite

Self-incompatibility (SI) mechanisms prevent self-fertilization in flowering plants based on specific discrimination between self-and non-self pollen. Since this trait promotes outcrossing and avoids inbreeding it is a widespread mechanism of controlling sexual plant reproduction. Growers and breeders have effectively exploited SI as a tool for manipulating domesticated crops for thousands of years. However, only within the past thirty years have studies begun to elucidate the underlying molecular features of SI. The specific S-determinants and some modifier factors controlling SI have been identified in the sporophytic system exhibited by Brassica species and in the two very distinct gametophytic systems present in Papaveraceae on one side and in Solanaceae, Rosaceae, and Plantaginaceae on the other. Molecular level studies have enabled SI to SC transitions (and vice versa) to be intentionally manipulated using marker assisted breeding and targeted approaches based on transgene integration, silencing, and more recently CRISPR knock-out of SI-related factors. These scientific advances have, in turn, provided a solid basis to implement new crop production and plant breeding practices. Applications of self-(in)compatibility include widely differing objectives such as crop yield and quality improvement, marker-assisted breeding through SI genotyping, and development of hybrids for overcoming intra-and interspecific reproductive barriers. Here, we review scientific progress as well as patented applications of SI, and also highlight future prospects including further elucidation of SI systems, deepening our understanding of SI-environment relationships, and new perspectives on plant self/non-self recognition.

show abstract

“…In summary, already at this early stage (Fernie and Yan, ), gene editing can be used to: activate or suspend the function of a gene (Qi et al ., ); create multiple different alleles of a gene (Rodriguez‐Leal et al ., ); edit any base (Gaudelli et al ., ); repair deletions (Dahan‐Meir et al ., ); add genes that do not exist in the original genome (Park et al ., ); and delete any sequence, including large chromosomal fragments or even entire chromosomes (Xiao et al ., ). CRISPR has successfully been used in many important crop species including, but not limited to, the cereals Hordeum vulgare (barley; Lawrenson et al ., ), Oryza sativa (rice; Shan et al ., ; Li et al , ), Triticum aestivum (wheat; Shan et al , ; Zhang et al ., ) and Zea mays (maize; Shi et al ., ), as well as Brassica oleracea (Lawrenson et al , ), Citrus (Peng et al ., ), Cucumis sativus (cucumber; Chandrasekaran et al ., ), Glycine max (soybean; Demorest et al ., ), Solanum lycopersicum (tomato; Cermak et al ., ), and Solanum tuberosum (potato; Clasen et al ., ; Ye et al ., ), with applications in novel species published on a monthly, if not weekly, basis. In terms of agricultural application, it is essential to realise that that the Cas9 enzyme is, in effect, a biological mutagen, a fact that needs to be conveyed more clearly to politicians as well as to the general public.…”

Section: The Advent and Adoption Of Genome Editing In Plantsmentioning

confidence: 99%

Metabolomics should be deployed in the identification and characterization of gene‐edited crops

et al. 2020

Self Cite

View full text Add to dashboard Cite

Gene-editing techniques are currently revolutionizing biology, allowing far greater precision than previous mutagenic and transgenic approaches. They are becoming applicable to a wide range of plant species and biological processes. Gene editing can rapidly improve a range of crop traits, including disease resistance, abiotic stress tolerance, yield, nutritional quality and additional consumer traits. Unlike transgenic approaches, however, it is not facile to forensically detect gene-editing events at the molecular level, as no foreign DNA exists in the elite line. These limitations in molecular detection approaches are likely to focus more attention on the products generated from the technology than on the process in itself. Rapid advances in sequencing and genome assembly increasingly facilitate genome sequencing as a means of characterizing new varieties generated by gene-editing techniques. Nevertheless, subtle edits such as single base changes or small deletions may be difficult to distinguish from normal variation within a genotype. Given these emerging scenarios, downstream 'omics' technologies reflective of edited affects, such as metabolomics, need to be used in a more prominent manner to fully assess compositional changes in novel foodstuffs. To achieve this goal, metabolomics or 'non-targeted metabolite analysis' needs to make significant advances to deliver greater representation across the metabolome. With the emergence of new edited crop varieties, we advocate: (i) concerted efforts in the advancement of 'omics' technologies, such as metabolomics, and (ii) an effort to redress the use of the technology in the regulatory assessment for metabolically engineered biotech crops.

show abstract

Generation of self-compatible diploid potato by knockout of S-RNase

Cited by 171 publications

References 22 publications

De Novo Domestication: An Alternative Route toward New Crops for the Future

De Novo Domestication: An Alternative Route toward New Crops for the Future

Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology

Metabolomics should be deployed in the identification and characterization of gene‐edited crops

Contact Info

Product

Resources

About