Generation of representative primary virus isolates from blood plasma after isolation of HIV-1 with CD44 MicroBeads

Cornelissen, Marion; Heeregrave, Edwin J.; Zorgdrager, Fokla; Pollakis, Georgios; Paxton, William A.; Kuyl, Antoinette C. van der

doi:10.1007/s00705-010-0806-x

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Among the patient plasma samples assayed, PSGL-1 was found to be present on all of the viruses tested and at similar levels to the positive control, CD44. Previous work has shown that CD44 can efficiently capture viruses from plasma of HIV-infected patients (49,50), and our results herein suggest that PSGL-1 could also be used for similar purposes in the future.…”

Section: Discussionsupporting

confidence: 66%

“…4). As an additional control, we also assayed CD44 incorporation in the same plasma samples, since CD44 is known to be incorporated with high efficiency into HIV-1 virions (49)(50)(51). CD44 was also incorporated into virions from all patient plasmas tested, with a wide range of virus input recovered by capture with anti-CD44 (8-87%), indicating that PSGL-1 falls into a category of host proteins that are incorporated with high efficiency into HIV virions.…”

Section: Psgl-1 Is Incorporated By a Broad Range Of Hiv-1 And Siv Isolates And Is Present Onmentioning

confidence: 99%

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The P-selectin ligand PSGL-1 (CD162) is efficiently incorporated by primary HIV-1 isolates and can facilitate trans-infection

Burnie

Persaud

Thaya

et al. 2021

Preprint

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While P-selectin glycoprotein ligand-1 (PSGL-1/CD162) has been studied extensively for its role in mediating leukocyte rolling through interactions with its receptor, P-selectin, recently, it was identified as a novel HIV-1 host restriction factor. One key mechanism of HIV-1 restriction is the ability of PSGL-1 to be physically incorporated into the external viral envelope, which effectively reduces infectivity by blocking virus attachment through the steric hindrance caused by its large ectodomain. Importantly, a large portion of the literature demonstrating the antiviral activity of PSGL-1 has utilized viruses produced in transfected cells which express high levels of PSGL-1. However, herein we show that virion-incorporated PSGL-1 is far less abundant on the surface of viruses produced via infection of physiologically relevant models (T cell lines and primary cells) compared to transfection (overexpression) models. Unique to this study, we show that PSGL-1 is incorporated in a broad range of HIV-1 and SIV isolates, supporting the physiological relevance of this incorporation. We also report that high levels of virion-incorporated PSGL-1 are detectable in plasma from viremic HIV-1 infected individuals, further corroborating the clinical relevance of PSGL-1 in natural infection. Additionally, we show that PSGL-1 on viruses is functionally active and can bind its cognate receptor, P-selectin, and that virions captured via P-selectin can subsequently be transferred to HIV-permissive bystander cells in a model of trans-infection. Taken together, our data suggest that PSGL-1 may have diverse roles in the physiology of HIV-1 infection, not restricted to the current antiviral paradigm.

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Section: Discussionsupporting

confidence: 66%

Section: Psgl-1 Is Incorporated By a Broad Range Of Hiv-1 And Siv Isolates And Is Present Onmentioning

confidence: 99%

The P-selectin ligand PSGL-1 (CD162) is efficiently incorporated by primary HIV-1 isolates and can facilitate trans-infection

Burnie

Persaud

Thaya

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Therefore, CD44 is likely incorporated into HIV-1 in vivo. In support of this notion, an anti-CD44 antibody and CD44 microbeads interact with viruses derived from the plasma of HIV-1 patients [22,76,97]. Interestingly, when HIV-1 is produced from CD44-expressing cells including PBMCs and hence incorporates CD44, cell-free infection of this HIV-1 is prevented by HA on CD44-expressing target CD4 + T cells at the step of virus attachment [98].…”

Section: Cd44 Promotes Fibroblastic Reticular Cell-mediated Trans-infectionmentioning

confidence: 92%

Roles of Virion-Incorporated CD162 (PSGL-1), CD43, and CD44 in HIV-1 Infection of T Cells

Murakami

Ono

2021

Viruses

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Nascent HIV-1 particles incorporate the viral envelope glycoprotein and multiple host transmembrane proteins during assembly at the plasma membrane. At least some of these host transmembrane proteins on the surface of virions are reported as pro-viral factors that enhance virus attachment to target cells or facilitate trans-infection of CD4+ T cells via interactions with non-T cells. In addition to the pro-viral factors, anti-viral transmembrane proteins are incorporated into progeny virions. These virion-incorporated transmembrane proteins inhibit HIV-1 entry at the point of attachment and fusion. In infected polarized CD4+ T cells, HIV-1 Gag localizes to a rear-end protrusion known as the uropod. Regardless of cell polarization, Gag colocalizes with and promotes the virion incorporation of a subset of uropod-directed host transmembrane proteins, including CD162, CD43, and CD44. Until recently, the functions of these virion-incorporated proteins had not been clear. Here, we review the recent findings about the roles played by virion-incorporated CD162, CD43, and CD44 in HIV-1 spread to CD4+ T cells.

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Proteomic Studies of HIV-1

Graham

2016

HIV-1 Proteomics

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Generation of representative primary virus isolates from blood plasma after isolation of HIV-1 with CD44 MicroBeads

Cited by 6 publications

References 18 publications

The P-selectin ligand PSGL-1 (CD162) is efficiently incorporated by primary HIV-1 isolates and can facilitate trans-infection

The P-selectin ligand PSGL-1 (CD162) is efficiently incorporated by primary HIV-1 isolates and can facilitate trans-infection

Roles of Virion-Incorporated CD162 (PSGL-1), CD43, and CD44 in HIV-1 Infection of T Cells

Proteomic Studies of HIV-1

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