Generation of reactive oxygen species in relevant cell lines as a bio-indicator of oxidative effects caused by acid mine water

Iji, Oluwafikemi T; Serem, June Cheptoo; Bester, Megan Jean; Venter, Eudri; Myburgh, Jan G.; McGaw, Lyndy Joy

doi:10.4314/wsa.v43i1.18

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…Fish occupy a high trophic level in the aquatic ecosystem and so they accumulate heavy metals from various sources including food, water, and sediments [131]. The response of fish to metal pollution varies with age and species of fish with the larvae being less tolerant to metals than the embryo since embryos have protective hard chorion layers and a perivitelline fluid that can impede the entry of metals [132][133]. Metal toxicity results in the development of oxidative stress through the stimulation of reactive oxygen species (ROS) production in the cells of fish, which stimulates the synthesis of antioxidant enzymes as a physiological protective mechanism to deal with and mitigate the negative impacts of metals.…”

Section: Metals and Fishmentioning

confidence: 99%

The Implications of AMD Induced Acidity, High Metal Concentrations and Ochre Precipitation on Aquatic Organisms

Ngole-Jeme,

Ndava

2023

Pol. J. Environ. Stud.

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Acid mine drainage (AMD) is a low pH, high conductivity, high metals, and sulphate ion concentration liquid formed by the oxidation of sulphide minerals in pyrite-bearing mine wastes. The generation of AMD has been associated mostly with coal and gold mining but mining of other sulphidic metal ores such as tin, silver, copper, and iron are also known to generate AMD [1][2][3]. A series of studies [4-9] have reported on AMD generation and the microbes involved in the process with all these studies indicating that AMD forms when sulphide-rich mine wastes get exposed to oxygen-rich conditions. Sulphur containing minerals associated with the generation of AMD according to Li et al. [10] and Chopard et al. [11] include pyrite (FeS 2 ), arsenopyrite (FeAsS), chalcopyrite (CuFeS 2 ), galena (PbS), gersdorffite (NiAsS), pyrrhotite, and sphalerite

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Section: Metals and Fishmentioning

confidence: 99%

The Implications of AMD Induced Acidity, High Metal Concentrations and Ochre Precipitation on Aquatic Organisms

Ngole-Jeme,

Ndava

2023

Pol. J. Environ. Stud.

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Metabolic activation of 2,4,6-trinitrotoluene; a case for ROS-induced cell damage

Adomako-Bonsu,

Jacobsen,

Maser

2024

Redox Biology

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Oxidative and haemostatic effects of copper, manganese and mercury, alone and in combination at physiologically relevant levels: An ex vivo study

et al. 2018

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Water contamination with metals due to anthropogenic activity is increasing and subsequent exposure increases the risk of associated toxicity. Exposure is not limited to a single metal but usually involves mixtures of different metals at different concentrations. Little is known about the contribution of this type of exposure, in humans, to the development of non-communicable diseases such as cardiovascular disease, and an increased risk to thrombosis. The World Health Organization has established limits for metal levels in drinking water and this includes levels for copper (Cu), manganese (Mn) and mercury (Hg). In this study, at 100X these limits, the ability of the metals’ oxidative effects as catalysts of the Fenton reaction and/or ability to bind glutathione (GSH) were determined. The haemostatic effects of these metals, alone and in combination, at the World Health Organization limit were then evaluated. The ultrastructural and viscoelastic alterations of exposed ex vivo whole blood were also evaluated using scanning electron microscopy and thromboelastography® (TEG), respectively. Cu, alone and in combination with Mn and/or Hg, induced hydroxyl radical formation and reduced GSH levels. Ex vivo exposure caused deformation of erythrocytes and accelerated platelet activation especially for Cu, alone and in combination, with Mn. Reduction in the lysis potential of the clot was also observed for all combinations, especially Cu in combination with Hg as well as Mn alone. Although the TEG findings were not statistically significant, the trends indicate that the exposure to these metals, alone and in combination, adversely affects thrombus formation in ex vivo blood, thereby potentially increasing the risk in exposed individuals for thrombosis.

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Generation of reactive oxygen species in relevant cell lines as a bio-indicator of oxidative effects caused by acid mine water

Cited by 3 publications

References 46 publications

The Implications of AMD Induced Acidity, High Metal Concentrations and Ochre Precipitation on Aquatic Organisms

The Implications of AMD Induced Acidity, High Metal Concentrations and Ochre Precipitation on Aquatic Organisms

Metabolic activation of 2,4,6-trinitrotoluene; a case for ROS-induced cell damage

Oxidative and haemostatic effects of copper, manganese and mercury, alone and in combination at physiologically relevant levels: An ex vivo study

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