Generation of reactive oxygen species and activity of platelet-activating factor acetylhydrolase in human monocyte-derived macrophages

Rußwurm, Stefan; Krause, S.; Finkelberg, L; Rühling, K.; Schauer, U. J. W.; Lösche, Wolfgang

doi:10.1016/0049-3848(94)90271-2

Cited by 16 publications

(9 citation statements)

References 32 publications

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“…In contrast, zymosan consistently stimulates ROS generation in macrophages (Russwurm et al, 1994;Bramble and Anderson, 1998) and thus was used as a positive ROS stimulus in our studies. Like zymosan stimulation, LPS significantly induced ROS generation in BMDM to a level similar to that of zymosan (Fig.…”

Section: Resultsmentioning

confidence: 80%

Pivotal Role of Reactive Oxygen Species in Differential Regulation of Lipopolysaccharide-Induced Prostaglandins Production in Macrophages

Zhao

Deng

et al. 2012

Mol Pharmacol

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Gram-negative bacterial endotoxin lipopolysaccharide (LPS) triggers the production of inflammatory cytokines, reactive oxygen species (ROS), and prostaglandins (PGs) by pulmonary macrophages. Here, we investigated if ROS influenced PGs production in response to LPS treatment in mouse bone marrow-derived macrophages (BMDM). We observed that pretreatment of BMDM with two structurally unrelated ROS scavengers, MnTMPyP and EUK-134, not only prevented LPS-induced ROS accumulation, but also attenuated the LPS-induced PGD 2 , but not PGE 2 , production. Conversely

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Section: Resultsmentioning

confidence: 80%

Pivotal Role of Reactive Oxygen Species in Differential Regulation of Lipopolysaccharide-Induced Prostaglandins Production in Macrophages

Zhao

Deng

et al. 2012

Mol Pharmacol

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“…HBMC were prepared by Ficoll-Paque density gradient centrifugation from venous blood of healthy male donors according to published procedures [14,15] and cultured in RPMI 1640 medium supplemented with 10% heat inactivated FCS, glutamine (2 mM), and penicillin (100 units/ml), streptomycin (100 ug/ml) in a humidified atmosphere (5% CO2 in air). The medium was changed daily.…”

Section: Cell Culturementioning

confidence: 99%

Modified low density lipoprotein delivers substrate for ceramide formation and stimulates the sphingomyelin‐ceramide pathway in human macrophages

Kinscherf¹,

Claus²,

Deigner³

et al. 1997

FEBS Letters

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“…Phagocytic NADH/NADPH oxidase is composed of at least 6 components: plasma membrane spanning cytochrome b558 composed of gp91 phox and p22 phox , the 3 cytosolic components p67 phox , p47 phox , p40 phox , and the small G protein rac. 12 15 The enhanced oxidative stress in inflammatory response might thus contribute to the pathogenesis of atherosclerosis. Systemic response to inflammation results in an increase in oxidized lipids in serum and in the enhancement of the oxidative modification of low-density lipoprotein.…”

mentioning

confidence: 99%

“…Various inflammatory cells such as macrophages and lymphocytes have a potency to generate ROS. 15 The enhanced oxidative stress in inflammatory response might thus contribute to the pathogenesis of atherosclerosis. Systemic response to inflammation results in an increase in oxidized lipids in serum and in the enhancement of the oxidative modification of low-density lipoprotein.…”

mentioning

confidence: 99%

Interaction of Oxidative Stress and Inflammatory Response in Coronary Plaque Instability

Kobayashi

Inoue

Ohashi

et al. 2003

ATVB

212

142

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Objective-C-reactive protein (CRP), a predictor of cardiovascular events, localizes in atherosclerotic arteries and exerts proinflammatory effects on vascular cells. Reactive oxygen species (ROS) have been implicated in atherogenesis and plaque instability. Methods and Results-Expressional pattern of CRP in directional coronary atherectomy specimens from 39 patients was examined. Characteristics of histological plaque instability and higher levels of serum CRP and fibrinogen were associated with the CRP immunoreactivity. In situ hybridization revealed the presence of CRP mRNA in coronary vasculature. Furthermore, the expression of CRP mRNA and protein was detected in cultured human coronary artery smooth muscle cells (CASMCs) by reverse transcriptase-polymerase chain reaction and Western blotting. In addition, CRP was frequently colocalized with p22 phox , an essential component of NADH/NADPH oxidase, which is an important source of ROS in vasculature. Moreover, the incubation of cultured CASMCs with CRP resulted in the enhanced p22 phox protein expression and in the generation of intracellular ROS. Conclusions-The expression of CRP in coronary arteries was associated with histological and clinical features of vulnerable plaque, and it had a prooxidative effect on cultured CASMCs, suggesting that it might play a crucial role in plaque instability and in the pathogenesis of acute coronary syndrome via its prooxidative effect. Key Words: C-reactive protein Ⅲ inflammation Ⅲ oxidative stress Ⅲ free radicals Ⅲ coronary artery diseases A therosclerosis is a chronic inflammatory disease. This concept is supported by recent findings where systemic inflammatory markers such as C-reactive protein (CRP) and fibrinogen are regarded as strong predictors of cardiovascular complications in various clinical settings. [1][2][3] Fibrinogen, a key coagulation factor, is considered to contribute atherogenesis by promoting platelet aggregation, fibrin formation, and plasma viscosity. 4 However, the role of CRP in the pathogenesis of atherosclerotic vascular diseases remains unknown. Recent histological investigations have demonstrated that CRP is present in the human arterial intima at atherosclerotic lesions and is frequently colocalized with the terminal complement complex. 5 Moreover, in vitro studies have shown that the stimulation of human endothelial cells with CRP induces the expression of adhesion molecules and monocyte chemoattractant protein-1 (MCP-1). 6,7 These data suggest that CRP might have direct proinflammatory effects on vascular cells which might, in part, explain the involvement of inflammation in atherogenesis.Reactive oxygen species (ROS) have been implicated in the pathogenesis of a variety of vascular diseases, including atherosclerosis. To date, many types of cells in vasculature have been shown to generate ROS. There are various potential sources that generate ROS in vascular cells: the mitochondrial electron transport chain, cyclooxygenase, lipoxygenase, xanthine oxidase, and NADH/NADPH oxidase. 8,9 Recent ...

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Generation of reactive oxygen species and activity of platelet-activating factor acetylhydrolase in human monocyte-derived macrophages

Cited by 16 publications

References 32 publications

Pivotal Role of Reactive Oxygen Species in Differential Regulation of Lipopolysaccharide-Induced Prostaglandins Production in Macrophages

Pivotal Role of Reactive Oxygen Species in Differential Regulation of Lipopolysaccharide-Induced Prostaglandins Production in Macrophages

Modified low density lipoprotein delivers substrate for ceramide formation and stimulates the sphingomyelin‐ceramide pathway in human macrophages

Interaction of Oxidative Stress and Inflammatory Response in Coronary Plaque Instability

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