Generation of reactive oxygen species induced by gold nanoparticles under x-ray and UV Irradiations

Misawa, Masaki; Takahashi, Junko

doi:10.1016/j.nano.2011.01.014

Cited by 296 publications

(220 citation statements)

References 25 publications

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“…It seems that in this case direct photodegradation of BSA is impossible. However, Au NCs can generate free radicals [46,47] or singlet oxygen [48]. This can lead to degradation of BSA.…”

Section: Discussionmentioning

confidence: 99%

Protein stabilized Au nanoclusters: spectral properties and photostability

Poderys¹,

Matulionytė-Safinė²,

Rupšys³

et al. 2016

Lith. J. Phys.

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Bovine serum albumin stabilized gold nanoclusters (BSA-Au nanoclusters) have been widely studied due to their possible applications in biomedicine as sensors, fluorescent or multi-modality markers, and therapeutic agents. Synthesis and optical properties of these nanoclusters have been extensively investigated; however, there is still very little data on photostability of BSA-Au nanoclusters. Photostability of BSA-Au nanoclusters is of major importance for a variety of applications, such as material sensing and fluorescence imaging. Herein we demonstrate that after synthesis the BSA-Au solution has two photoluminescence (PL) bands peaking at 468 and 660 nm. Nevertheless, a different behaviour of the PL bands at 468 and 660 nm upon irradiation indicates that only band at 660 nm is related to PL of Au nanoclusters. BSA-Au nanoclusters exhibit great colloidal stability and do not undergo irreversible changes when heated up to 65 °C. However, irradiation of BSA-Au nanoclusters causes a wavelength dependent decrease of intensity and a hypsochromic shift of the PL band at 660 nm which is proportional to the delivered dose. The shift of the PL band at 660 nm could occur due to loss of several gold atoms in Au nanoclusters and/or due to deterioration of a nanoparticle coating layer. We have also demonstrated that the photostability of BSA-Au nanoclusters increases in the cell growth medium.

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“…It seems that in this case direct photodegradation of BSA is impossible. However, Au NCs can generate free radicals [46,47] or singlet oxygen [48]. This can lead to degradation of BSA.…”

Section: Discussionmentioning

confidence: 99%

Protein stabilized Au nanoclusters: spectral properties and photostability

Poderys¹,

Matulionytė-Safinė²,

Rupšys³

et al. 2016

Lith. J. Phys.

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“…However, the mechanism of action leading to increased radiation-induced cell death mediated by NP is not yet fully understood. An increased generation of reactive oxygen species by secondary water radiolysis (29) as well as a potential role of mitochondria as biological mechanisms contributing to NP-mediated enhancement of radiation treatment (30) have been discussed. A direct functional proof for enhanced dose delivery could be the analysis of initial DNA damage foci, such as gamma-H2AX or 53BP1, in tumour and neighbouring normal tissue of an experimental model system.…”

Section: Biological Pathways Of Treatment Responsementioning

confidence: 99%

Targeted nanoparticles for tumour radiotherapy enhancement—the long dawn of a golden era?

Gargioni¹,

Schulz²,

Raabe³

et al. 2016

Ann. Transl. Med.

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Despite considerable progress in (I) our understanding of the aetiopathology of head and neck cancer and (II) the precise delivery of radiotherapy, long-term survival rates for many patients with head and neck cancer remain disappointingly low. Over the past years, gold nanoparticles (NP) have emerged as promising radiation dose enhancers. In a recent study published in Nanoscale, Popovtzer et al. have used gold NP coated with an antibody against the epidermal growth factor receptor (EGFR) in an attempt to enhance radiation-induced tumour cell killing in a head and neck cancer xenograft model. They report a significant impact of the combined treatment with radiation and gold NP on tumour growth and suggest an involvement of apoptosis, inhibition of angiogenesis and diminished tissue repair. In this perspective, we illustrate the underlying radiobiophysical concepts and discuss some of the challenges associated with this and related nanoparticle-radiotherapy studies from a physics, chemistry, biology and therapy angle. We conclude that strong interdisciplinary collaborations spanning all these areas are crucially important to proceed towards effective cancer treatment with gold NP "from bench to bedside".

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“…1. Schematic diagram of the apparatus with the main components detailed: [1] Stage Datum Laser Gate, [2] Multiwell Microplate, [3] Nozzle Carousel, [4] Droplet Detector, [5] Waste Arm, [6] Carousel Datum. a) Electronic mail: cpolin01@qub.ac.uk ber of applications including cancer radiotherapy 1 , civil nuclear power 2 , and the semiconductor industry 3 .…”

Section: A Novel Experimental Approach To Investigate Radiolysis Procmentioning

confidence: 99%

“…The droplet falls through an opto-interrupter before it reaches the multiwell plate, alerting the software to deactivate the syringe motor. [1] is connected to the syringe plunger with an adaptor [2], allowing it to both push and pull. A 'C'-section support beam [3] holds the actuator above the driver syringe [4].…”

Section: A Operational Protocolmentioning

confidence: 99%

A novel experimental approach to investigate radiolysis processes in liquid samples using collimated radiation sources

Polin

Wardlow

McQuaid

et al. 2015

Review of Scientific Instruments

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Here is detailed a novel and low-cost experimental method for high-throughput automated fluid sample irradiation. The sample is delivered via syringe pump to a nozzle, where it is expressed in the form of a hanging droplet into the path of a beam of ionising radiation. The dose delivery is controlled by an upstream lead shutter, which allows the beam to reach the droplet for a user defined period of time. The droplet is then further expressed after irradiation until it falls into one well of a standard microplate. The entire system is automated and can be operated remotely using software designed in-house, allowing for use in environments deemed unsafe for the user (synchrotron beamlines, for example). Depending on the number of wells in the microplate, several droplets can be irradiated before any human interaction is necessary, and the user may choose up to 10 samples per microplate using an array of identical syringe pumps, the design of which is described here. The nozzles consistently produce droplets of 25.1 ± 0.5 μl.

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Generation of reactive oxygen species induced by gold nanoparticles under x-ray and UV Irradiations

Cited by 296 publications

References 25 publications

Protein stabilized Au nanoclusters: spectral properties and photostability

Protein stabilized Au nanoclusters: spectral properties and photostability

Targeted nanoparticles for tumour radiotherapy enhancement—the long dawn of a golden era?

A novel experimental approach to investigate radiolysis processes in liquid samples using collimated radiation sources

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