Abstract:Endothelial progenitor cells (EPC), bone marrow derived cells circulating in adult peripheral blood, may play an important role in postnatal vasculogenesis. We have used two different culture conditions for the isolation and generation of EPC from peripheral blood. Mononuclear cells (MNC) were harvested from peripheral blood (PB) of healthy volunteers by Ficoll-density gradient centrifugation. In the first method according to Hill et al., NEJM2003, 348: 593, cells were plated at a density of 5x106 cells/well i… Show more
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