Generation of pluripotent stem cells via protein transduction

Li, Xia; Zhang, Pengfei; Wei, Chao; Zhang, Yunhai

doi:10.1387/ijdb.140007xl

Cited by 24 publications

(12 citation statements)

References 73 publications

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“…iPSC generation methodology has improved with different delivery systems, including non-integrating vectors, deletion after integration, DNA-free transduction, and chemical induction. [5][6][7][8][9][10][11] Furthermore, novel approaches for iPSC production have been developed, including combinations of alternative transcription factors. 12 In addition to those, reprogramming-susceptible cell types have been identified.…”

Section: Reprogramming Of Somatic Cellsmentioning

confidence: 99%

Mechanism of human somatic reprogramming to iPS cell

Teshigawara

Cho

Kameda

et al. 2017

Laboratory Investigation

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Somatic reprogramming to induced pluripotent stem cells (iPSC) was realized in the year 2006 in mice, and in 2007 in humans, by transiently forced expression of a combination of exogenous transcription factors. Human and mouse iPSCs are distinctly reprogrammed into a 'primed' and a 'naïve' state, respectively. In the last decade, puzzle pieces of somatic reprogramming have been collected with difficulty. Collectively, dissecting reprogramming events and identification of the hallmark of sequentially activated/silenced genes have revealed mouse somatic reprogramming in fragments, but there is a long way to go toward understanding the molecular mechanisms of human somatic reprogramming, even with developing technologies. Recently, an established human intermediately reprogrammed stem cell (iRSC) line, which has paused reprogramming at the endogenous OCT4-negative/exogenous transgene-positive pre-MET (mesenchymal-to-epithelial-transition) stage can resume reprogramming into endogenous OCT4-positive iPSCs only by change of culture conditions. Genome-editing-mediated visualization of endogenous OCT4 activity with GFP in living iRSCs demonstrates the timing of OCT4 activation and entry to MET in the reprogramming toward iPSCs. Applications of genome-editing technology to pluripotent stem cells will reshape our approaches for exploring molecular mechanisms.

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Section: Reprogramming Of Somatic Cellsmentioning

confidence: 99%

Mechanism of human somatic reprogramming to iPS cell

Teshigawara

Cho

Kameda

et al. 2017

Laboratory Investigation

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“…Kim and colleagues generated iPSCs using recombinant reprogramming factors obtained from a stable HEK293 human cell line. However, it required several rounds of transduction and 8 weeks to achieve cellular reprogramming [32,39].…”

Section: Methods Of Cellular Reprogrammingmentioning

confidence: 99%

Cell reprogramming and neuronal differentiation applied to neurodegenerative diseases: Focus on Parkinson's disease

et al. 2015

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a b s t r a c tAdult cells from patients can be reprogrammed to induced pluripotent stem cells (iPSCs) which successively can be used to obtain specific cells such as neurons. This remarkable breakthrough represents a new way of studying diseases and brought new therapeutic perspectives in the field of regenerative medicine. This is particular true in the neurology field, where few techniques are amenable to study the affected tissue of the patient during illness progression, in addition to the lack of neuroprotective therapies for many diseases.In this review we discuss the advantages and unresolved issues of cell reprogramming and neuronal differentiation. We reviewed evidence using iPSCs-derived neurons from neurological patients. Focusing on data obtained from Parkinson's disease (PD) patients, we show that iPSC-derived neurons possess morphological and functional characteristics of this disease and build a case for the use of this technology to study PD and other neuropathologies while disease is in progress. These data show the enormous impact that this new technology starts to have on different purposes such as the study and design of future therapies of neurological disease, especially PD.

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“…78 Thus, iPSCs could be a promise for disease modeling, drug selection and cell therapies in both regenerative medicine and agriculture. 79 The generation of iPSCs is slow and inefficient comparing with SCNT, but iPSCs are much easier to obtain. The iPSCs are commonly derived from somatic cells by transfecting two pluripotent transcription factors, Oct4 and Sox2 (sex determining region Y box 2), and two protooncogenes, c-Myc (v-Myc myelocytomatosis avian viral oncogene homolog) and Klf4 (Krueppel-like factor 4).…”

Section: Reprogramming Gene Expressionmentioning

confidence: 99%

Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer

Simões

Santos

2017

Organogenesis

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Somatic cell nuclear transfer is a technique to create an embryo using an enucleated oocyte and a donor nucleus. Nucleus of somatic cells must be reprogrammed in order to participate in normal development within an enucleated egg. Reprogramming refers to the erasing and remodeling of cellular epigenetic marks to a lower differentiation state. Somatic nuclei must be reprogrammed by factors in the oocyte cytoplasm to a rather totipotent state since the reconstructed embryo must initiate embryo development from the one cell stage to term. In embryos reconstructed by nuclear transfer, the donor genetic material must respond to the cytoplasmic environment of the cytoplast and recapitulate this normal developmental process. Enucleation is critically important for cloning efficiency because may affect the ultrastructure of the remaining cytoplast, thus resulting in a decline or destruction of its cellular compartments. Nonetheless, the effects of in vitro culturing are yet to be fully understood. In vitro oocyte maturation can affect the abundance of specific transcripts and are likely to deplete the developmental competence. The epigenetic modifications established during cellular differentiation are a major factor determining this low efficiency as they act as epigenetic barriers restricting reprogramming of somatic nuclei. In this review we discuss some factors that could impact cell differentiation in embryo generated by nuclear transfer.

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Generation of pluripotent stem cells via protein transduction

Cited by 24 publications

References 73 publications

Mechanism of human somatic reprogramming to iPS cell

Mechanism of human somatic reprogramming to iPS cell

Cell reprogramming and neuronal differentiation applied to neurodegenerative diseases: Focus on Parkinson's disease

Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer

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