Generation of Octaploid Switchgrass by Seedling Treatment with Mitotic Inhibitors

Yoon, Sangwoong; Aucar, Sheyla; Hernlem, Bradley J.; Edmé, Serge J.; Palmer, Nathan A.; Sarath, Gautam; Mitchell, Robert B.; Blumwald, Eduardo; Tobias, Christian M.

doi:10.1007/s12155-016-9795-2

Cited by 8 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase of photosynthesis can encourage the increase in weight, as already mentioned. Increase in the weight of seed has also been associated with induced polyploidy [47]. What can potentiate the vigor and germination of seeds, favoring the formation of a more homogeneous stand.…”

Section: Discussionmentioning

confidence: 99%

“…The increase of photosynthesis is attributed to the increase of the tissues, increased capacity for storage of CO 2 , and increase of gs . The size of the epidermal cells, cells, can also be associated with the ploidy level of the material under observation [47]. In addition to the increase in the activity of multiple enzymes such as hydrolases and expansins [64].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anatomical tool in the identification of ploids in maize seedlings and potential use in initial stage of double haploides obtainment process

Pires

Souza

Vilela

et al. 2019

Preprint

View full text Add to dashboard Cite

27Studies that optimize the haploid technique in the removal of maize lines are necessary. 28Between the stages that mostly requires attention and it is directly related to the success of 29 the technology is the correctly separation of induced haploids and diploids. Morphological 30 markers are commonly used but have strong influence of the environment, and laboratory 31 methods have been developed and may be more efficient. Thus, the objective was to study 32 the use of the anatomical analysis tool, through the analysis of young maize leaf for use as 33 the indirect markers in the identification of ploidys. The hybrids were crossed with the KEMS 34 haploid inducer. The seeds crossed, were selected according to the R-navajo marker and 35 submitted to two different protocols of chromosome duplication. Plants that survived to the 36 duplication protocols were acclimated in greenhouse and then transferred to the field. After 37 the self-polinization of the DH0 plants, the DH1 seeds were taken to the field, divided into 38 treatments according to the parentals and duplication protocols. At the vegetative stage V4 of 39 the plants, leaf tissue samples were collected to the evaluation of the amount of DNA and 40 identification of ploidys and anatomical analysis. The nuclear DNA review of each sample 41 was performed for the comparison in histograms of the position of G1 peak to the G1 peak of 42 the internal or external reference standard. A high accuracy came to validate an anatomical 43 tool, through the variables studied in this work, as a marker in the differentiation of ploidis in 44 maize plants, and it can be used in selection programs. The anatomy made in some letters is 45 a non-destructible technique and, together with a flow cytometry technique, can be used as 46 an indirect method in haploid cutting programs at the initial stage of the identification of 47 seedlings. 48 49 50 51 3 52

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anatomical tool in the identification of ploids in maize seedlings and potential use in initial stage of double haploides obtainment process

Pires

Souza

Vilela

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…mexicana) hybrids resulted in higher seed yield than that of diploid crosses when subjected to selection for the regular bivalent formation in maize [16]. Caryopsis and cell size of octoploid Panicum virgatum L. increased and the anatomy of the leaf tissues was changed when compared with diploid individuals, while fertility of the induced plants was decreased significantly [58]. Allotriploid hybrids of Miscanthus × giganteus were sterile but had higher biomass potential and persistence when compared with diploids.…”

Section: Restoration Of Fertilitymentioning

confidence: 99%

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Rauf¹,

Ortíz²,

Malinowski³

et al. 2021

Preprint

View full text Add to dashboard Cite

Polyploidy is a condition of having more than two sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological methods (2n gametes). It is considered an interesting approach due to increased genescope and expression, thus resulting in phenotypic changes, such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages from optimized application methods, concentration and time mainly using colchicine. These experimental polyploids proved to be a valuable tool for understanding gene expression that is driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers aided on the identification of rare alleles for particular loci when compared with diploids, which may explain their heterozygosity, phenotypic plasticity and adaptability to diverse environments. It has also been observed that experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g. leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids underwent selection for several generations to improve their performance and stability.

show abstract

“…Kuntze) hybrids resulted in higher seed yield than that of diploid crosses when subjected to selection for the regular bivalent formation in maize [16]. Caryopsis and cell size of octoploid switchgrass (Panicum virgatum L.) increased and the anatomy of the leaf tissues was changed when compared with diploid individuals, while fertility of the induced plants was decreased significantly [33]. Allotriploid hybrids of giant miscanthus (Miscanthus × giganteus J.M.Greef, Deuter ex Hodk., Renvoize) were sterile but had higher biomass potential and persistence than diploids.…”

Section: Restoration Of Fertilitymentioning

confidence: 99%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

View full text Add to dashboard Cite

Polyploidy means having more than two basic sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological (2n gametes) methods. This approach allows an increased gene scope and expression, thus resulting in phenotypic changes such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects that are partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages after optimizing methods, concentration and time, particularly when using colchicine. These experimental polyploids are a valuable tool for understanding gene expression, which seems to be driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers facilitated the identification of rare alleles for particular loci when compared with diploids, and also explained their heterozygosity, phenotypic plasticity and adaptability to diverse environments. Experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g., leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids should undergo selection for several generations to improve their performance and stability.

show abstract

Generation of Octaploid Switchgrass by Seedling Treatment with Mitotic Inhibitors

Cited by 8 publications

References 35 publications

Anatomical tool in the identification of ploids in maize seedlings and potential use in initial stage of double haploides obtainment process

Anatomical tool in the identification of ploids in maize seedlings and potential use in initial stage of double haploides obtainment process

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Induced Polyploidy: A Tool for Forage Species Improvement

Contact Info

Product

Resources

About