Generation of Novel Hybrid Aptamer–Molecularly Imprinted Polymeric Nanoparticles

Poma, Alessandro; Brahmbhatt, Heli; Pendergraff, Hannah M.; Watts, Jonathan K.; Turner, Nicholas W.

doi:10.1002/adma.201404235

Cited by 75 publications

(47 citation statements)

References 64 publications

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“…However, Poma et al. argued that aptamer binding needs correct folding, and a single terminal anchor may confer much flexibility leading to reduced binding affinity . They reported a nucleotide modification approach in 2015.…”

Section: Functional Dna: Aptamers and Dnazymesmentioning

confidence: 99%

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Molecular Imprinting with Functional DNA

Zhang

Liu

2019

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Molecular imprinting refers to templated polymerization with rationally designed monomers, and this is a general method to prepare stable and cost‐effective ligands. This attractive concept however suffers from low affinity, low specificity, and limited signaling mechanisms for binding. Acrydite‐modified DNA oligonucleotides can be readily copolymerized into acrylic polymers. With molecular recognition and catalytic functions, such functional DNAs are recently shown to enhance the performance of molecularly imprinted polymers (MIPs) in a few ways. First, DNA aptamers are used as macromonomers to enhance binding affinity and specificity of MIPs. Second, DNA can help produce optical signals to follow binding events. Third, imprinting can also improve the performance of catalytic DNA by enhancing its activity and specificity toward the template substrate. Finally, MIP is shown to help aptamer selection. Bulk imprinting, nanoparticle imprinting, and surface imprinting are all demonstrated with DNA. Since both DNA and synthetic polymers are cost effective and stable, their hybrid materials still possess such properties while enhancing the function of each component. This review covers recent developments on the abovementioned aspects of DNA‐containing MIPs, a field just emerged in the last five years, and future research directions are discussed toward the end.

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Section: Functional Dna: Aptamers and Dnazymesmentioning

confidence: 99%

“…B) List of polymerizable DNA sequences used in this work: the cocaine aptamer with one (T*1) or six (T*6) polymerizable residues or with a flexible 5′‐terminal acrydite. All panels reproduced with permission . Copyright 2015, Wiley‐VCH.…”

Section: Functional Dna: Aptamers and Dnazymesmentioning

confidence: 99%

Molecular Imprinting with Functional DNA

Zhang

Liu

2019

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“…In this process, it is essential that the template interacting with the monomer does not deactivate the polymerization positions. The very high versatility offered by the chemical polymerization strategy [8][9][10] is difficult to transfer to polymerization that is electrochemically driven, as the availability of an electroactive monomer is introduced as a constraint. Pyrrole or (di)-thiophene are frequently used for conductive MIP preparation [11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…In the rebinding process, the solvation of the 3D imprinted cavity modulates the interactions with the template [20]. Recently, however, some important advances in affinity and specificity of soluble imprinted nano-polymers have been reported [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Selectivity and Efficiency of Conductive Molecularly Imprinted Polymer (c-MIP) Based on 5-Phenyl-Dipyrromethane and 5-Phenol-Dipyrromethane for Quorum Sensing Precursors Detection

Susmel

Comuzzi

2017

Chemosensors

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Functional polymers that selectively recognize target compounds are developed by imprinting polymerization. In the present paper, two different dipyrromethanes, 5-phenoldipyrromethane (5-pOH-DP) and 5-phenyl-dipyrromethane (5-ph-DP), are synthetized and investigated to develop conductive molecularly imprinted polymer (cMIP) sensors. As target molecules, two homoserine lactone derivatives were templated by an electrochemically driven polymerization process. Acyl-homoserine lactones (AHLs), also called homoserine lactones (HS), are a class of signaling molecules involved in bacterial quorum sensing (QS), which is a strategy of coordination among bacteria mediated by population density. The preparation of cMIP from 5-pOH-DP and 5-ph-DP in the presence of acetyl-homoserine lactone (Acetyl-HS) or carboxybenzyl-homoserine lactone (Cbz-HS) was performed by cyclic voltammetry (CV). The cMIP selectivity and sensitivity were assessed by microgravimetry (QCM). Both series of measurements were performed with the aid of an Electrochemical Quartz Crystal Microbalance (EQCM/QCM). The experimental evidences are discussed with respect to NMR measurements that were conducted to gain insight into the interactions established between monomers and templates. The NMR data interpretation offers preliminary information about the most probable positions involved in interaction development for both molecules and highlights the role of the hydration shell. The QCM-cMIP sensor was able to detect the analyte in the linear range from 10 −8 mol·L −1 to 10 −6 mol·L −1 and a limit of detection (LOD) of 22.3 ng (3σ of the blank signal) were evaluated. QCM rebinding tests demonstrated that cMIP selectivity was driven by the pendant group of dipyrromethane, which was also confirmed by the NMR data.

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“…Molecular imprinting technology (MIT), concerned with the construction of polymer cavity with the memory of the template, has long been identified as a common and cost‐effective approach to synthesize imprinted materials for small molecule, protein, virus, and even bacterial, mammalian cell recognition. To date, protein imprinted materials have been employed for myriad applications in separation, sensing, biomimetic catalysis, and protein crystallization .…”

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confidence: 99%

Programmable Downregulation of Enzyme Activity Using a Fever and NIR‐Responsive Molecularly Imprinted Nanocomposite

Zhang

Wang

et al. 2015

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A fever and NIR-responsive molecularly imprinted nanocomposite is designed for programmable downregulation of enzyme activity. The target enzyme can be captured specifically and its activity can be downregulated only when body temperature increases abnormally. Upon NIR irradiation, the temperature of the destination region can increase accordingly inducing a further decrease in the enzyme activity.

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Generation of Novel Hybrid Aptamer–Molecularly Imprinted Polymeric Nanoparticles

Cited by 75 publications

References 64 publications

Molecular Imprinting with Functional DNA

Molecular Imprinting with Functional DNA

Selectivity and Efficiency of Conductive Molecularly Imprinted Polymer (c-MIP) Based on 5-Phenyl-Dipyrromethane and 5-Phenol-Dipyrromethane for Quorum Sensing Precursors Detection

Programmable Downregulation of Enzyme Activity Using a Fever and NIR‐Responsive Molecularly Imprinted Nanocomposite

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