Generation of neural progenitors from induced Bama miniature pig pluripotent cells

Abstract

Pig pluripotent cells may represent an advantageous experimental tool for developing therapeutic application in the human biomedical field. However, it has previously been proven to be difficult to establish from the early embryo and its pluripotency has not been distinctly documented. In recent years, induced pluripotent stem (iPS) cell technology provides a new method of reprogramming somatic cells to pluripotent state. The generation of iPS cells together with or without certain small molecules has become a routine technique. However, the generation of iPS cells from pig embryonic tissues using viral infections together with small molecules has not been reported. Here, we reported the generation of induced pig pluripotent cells (iPPCs) using the iPS technology in combination with valproic acid (VPA). VPA treatment significantly increased the expression of pluripotent genes and played an important role in early reprogramming. We showed that iPPCs resembled pig epiblast cells in their morphology and pluripotent markers, such as OCT4, NANOG, and SSEA1. It had a normal karyotype and could form embryoid bodies, which express three germ layer markers in vitro. In addition, the iPPCs might directly differentiate into neural progenitors after being induced with the retinoic acid and extracellular matrix. Our study established a reasonable method to generate pig pluripotent cells, which might be a new donor cell source for human neural disease therapy.

Culture of iPSCs Derived Pancreatic β-Like Cells In Vitro Using Decellularized Pancreatic Scaffolds: A Preliminary Trial

“…Nude mouse tumorigenicity assay proved the multipotent capacity of the iPSCs [29, 30]. For the past few years, iPSCs have been successfully differentiated into neurons [31], cardiac muscle cells [32], and hematopoietic cells [33] among other cell types. While the current most effective strategy to cure diabetes is islet or β cell transplantation, the shortage of donor, low survival rate of the grafted cells, and immune rejection following transplantation limit the success of this approach.…”

Isolation and Culture of Pig Spermatogonial Stem Cells and Their in Vitro Differentiation into Neuron-Like Cells and Adipocytes

“…Their rarity has presented problems in isolation and culturing them and the development of successful techniques for doing so has been the focus of intense study. At present, SSCs have been successfully isolated and cultured from many species, including human, mice [ 5 ], cattle [ 6 ], pig [ 7 , 8 , 9 ], goat [ 10 , 11 , 12 ], dog [ 13 ], chicken [ 14 , 15 ], and even fish [ 16 ].…”

Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes

“…The iPS technique allows researchers to establish stem cells by introducing several reprogramming factors into the cells [Takahashi and Yamanaka, ; Takahashi et al, ]. The porcine iPS cells have been established by various scientists, and the characteristics of these stem cells were reported in ∼20 articles [Esteban et al, , ; Ezashi et al, , ; Wu et al, ; West et al, ; Huang et al, ; Ruan et al, ; Aravalli et al, ; Cheng et al, ; Liu et al, ; Rodriguez et al, ; Fujishiro et al, ; Ji et al, ; Kues et al, ; Li et al, ; Park et al, ,; Petkov et al, ; Ma et al, ]. The morphology of the porcine iPS cells is close to that of their human counterparts, which was described as the “primed state” in previous reports.…”