Generation of Multispecies Oral Bacteria Biofilm Models

Brown, Jason L.; Butcher, Mark C.; Veena, Chandra Lekha Ramalingam; Chogule, Safa; Johnston, William; Ramage, Gordon

doi:10.1007/978-1-0716-2780-8_12

Cited by 1 publication

(1 citation statement)

References 28 publications

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“…Biofilm formation progress by co-aggregation of a number of different additional species, depending on the surface properties, nutrient quality and availability, oxygen levels and microbial interactions including intra and inter-regnum interactions [ 53 ]. Although polymicrobial in-vitro models are reported in the literature [ 53 , 54 , 55 ], major technical limitations occur in mimicking the complexity of the peri-implant microbiota using reliable and reproducible in vitro biofilm models, encompassing the stringent culturing conditions of most of the oral microorganisms [ 56 , 57 ] and moving from two-dimensional to novel three-dimensional biofilm models [ 58 ]. Overall, further studies are needed to evaluate the long-term effectiveness and safety of R89BS coating in more complex environments, in in vivo conditions and finally in clinical settings.…”

Section: Discussionmentioning

confidence: 99%

Rhamnolipid 89 Biosurfactant Is Effective against Streptococcus oralis Biofilm and Preserves Osteoblast Behavior: Perspectives in Dental Implantology

Tambone,

Ceresa,

Marchetti

et al. 2023

IJMS

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Biofilm-related peri-implant diseases represent the major complication for osteointegrated dental implants, requiring complex treatments or implant removal. Microbial biosurfactants emerged as new antibiofilm coating agents for implantable devices thanks to their high biocompatibility. This study aimed to assess the efficacy of the rhamnolipid 89 biosurfactant (R89BS) in limiting Streptococcus oralis biofilm formation and dislodging sessile cells from medical grade titanium, but preserving adhesion and proliferation of human osteoblasts. The inhibitory activity of a R89BS coating on S. oralis biofilm formation was assayed by quantifying biofilm biomass and microbial cells on titanium discs incubated up to 72 h. R89BS dispersal activity was addressed by measuring residual biomass of pre-formed biofilms after rhamnolipid treatment up to 24 h. Adhesion and proliferation of human primary osteoblasts on R89BS-coated titanium were evaluated by cell count and adenosine-triphosphate quantification, while cell differentiation was studied by measuring alkaline phosphatase activity and observing mineral deposition. Results showed that R89BS coating inhibited S. oralis biofilm formation by 80% at 72 h and dislodged 63–86% of pre-formed biofilms in 24 h according to concentration. No change in the adhesion of human osteoblasts was observed, whereas proliferation was reduced accompanied by an increase in cell differentiation. R89BS effectively counteracts S. oralis biofilm formation on titanium and preserves overall osteoblasts behavior representing a promising preventive strategy against biofilm-related peri-implant diseases.

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