Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit

Abstract

Monoclonal antibodies offer high specificity and this makes it an important tool for molecular biology, biochemistry and medicine. Typically, monoclonal antibodies are generated by fusing mouse spleen cells that have been immunized with the desired antigen with myeloma cells to create immortalized hybridomas. Here, we describe the generation of monoclonal antibodies that are specific to Chikungunya virus using ClonaCell-HY system.

Hybridoma technology a versatile method for isolation of monoclonal antibodies, its applicability across species, limitations, advancement and future perspectives

“…A fusion of human B cells with different fusion partners limits the use of these mAbs for therapeutic applications. Several hetero-myelomas fusion has been successfully employed for the generation of mAbs of human origin for different diseases like HIV [28,55], Chikungunya [56,57], Dengue [58] etc. However, such hybridomas are Fig.…”

“…In this strategy, they immunized the mice with transfected cells overexpressing PDPN and screened the fused hybridoma cells using flow cytometry. These cell-based immunization methods are useful in the isolation of mAbs against various membrane proteins which is still difficult to achieve otherwise [56,57].…”

“…It has also facilitated the use of murine hybridoma technology in a less cumbersome and user friendly. Methylcellulosebased semi-solid selective medium is preferentially used in hybridoma selection [56]. Technically it avoids the loss of rare clones from an overgrowth of faster-growing cells, which can occur during selection in a liquid medium.…”