Generation of mice deficient in RNA-binding motif protein 3 (RBM3) and characterization of its role in innate immune responses and cell growth

Matsuda, Atsushi; Ogawa, Masahiro; Yanai, Hideyuki; Naka, Daiji; Goto, Atsuko; Ao, Tomoka; Tanno, Yuji; Takeda, Kiyoshi; Watanabe, Yoshinori; Honda, Kenya; Taniguchi, Tadatsugu

doi:10.1016/j.bbrc.2011.06.038

Cited by 28 publications

(25 citation statements)

References 28 publications

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“…These conditions are present in the environment of a malignant tumour [31]. The role of RBM3 in adverse growth conditions is to increase cell survival by means of hindering the normal decrease of protein synthesis and promoting cells to continue through the cell cycle [16, 32]. It has the making of a powerful oncoprotein, but is linked to an improved survival.…”

Section: Discussionmentioning

confidence: 99%

Podocalyxin-like and RNA-binding motif protein 3 are prognostic biomarkers in urothelial bladder cancer: a validatory study

et al. 2017

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Background: Urothelial bladder cancer (UBC) is a disease that often is discovered when the tumour is non-muscle invasive, i.e. in Ta or T1 stage. Some patients will progress into muscle-invasive disease, a potentially deadly condition. Although there are some prognostic models, the need for prognostic and predictive biomarkers is considerate and urgent. Membranous expression of podocalyxin-like protein 1 (PODXL) and low expression of the RNA-binding motif 3 (RBM3) has previously been shown to be associated with an aggressive tumour phenotype and poor prognosis in several forms of cancer, including UBC. In this study, we sought to validate the prognostic impact of PODXL and RBM3 in an independent cohort of UBC.

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Section: Discussionmentioning

confidence: 99%

Podocalyxin-like and RNA-binding motif protein 3 are prognostic biomarkers in urothelial bladder cancer: a validatory study

et al. 2017

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“…RBM3 -KO mice 59 was kindly provided by Prof. T. Taniguchi, the University of Tokyo. The mutant mice were backcrossed to C57BL/6 J wild-type mice at least seven generations before being used in the experiments.…”

Section: Methodsmentioning

confidence: 99%

TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM3

Fujita

Higashitsuji

Liu

et al. 2017

Sci Rep

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Cold-inducible RNA-binding protein (CIRP) and RNA-binding motif protein 3 (RBM3) are two evolutionarily conserved RNA-binding proteins that are structurally related to hnRNPs and upregulated in response to moderately low temperatures in mammalian cells. Although contributions of splicing efficiency, the gene promoters activated upon mild hypothermia and the transcription factor Sp1 to induction of CIRP have been reported, precise mechanisms by which hypothermia and other stresses induce the expression of mammalian cold-inducible proteins (CIPs) are poorly understood. By screening the serine/arginine-rich splicing factors (SRSFs), we report that the transcript and protein levels of SRSF5 were increased in mammalian cells cultured at 32 °C. Expression of SRSF5 as well as CIRP and RBM3 were also induced by DNA damage, hypoxia, cycloheximide and hypotonicity. Immunohistochemical studies demonstrated that SRSF5 was constitutively expressed in male germ cells and the level was decreased in human testicular germ cell tumors. SRSF5 facilitated production of p19 H-RAS, and increased sensitivity to doxorubicin in human U-2 OS cells. Induction of CIPs was dependent on transient receptor potential vanilloid 4 (TRPV4) channel protein, but seemed independent of its ion channel activity. These findings indicate a previously unappreciated role for the TRP protein in linking environmental stress to splicing.

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“…Indeed, expression of RBM3 is required for appropriate cell cycle progression and preventing mitotic catastrophe by increasing mRNA stability translation of rapidly degraded transcripts [12]. The G2-phase control role of RBM3 was uncovered in RBM3 silenced A2780 cells [13] and RBM3 knockout mice [14]. What's more, the significant increasing expression of RBM3 in old muscle revealed that it shows the ability to maintain or restore muscle mass during prolonged periods of disuse [15].…”

Section: Introductionmentioning

confidence: 99%

RNA binding motif protein 3: a potential biomarker in cancer and therapeutic target in neuroprotection

Zhou

Zhang

et al. 2017

Oncotarget

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RNA binding motif 3 (RBM3) is a highly conserved cold-induced RNA binding protein that is transcriptionally up-regulated in response to harsh stresses. Featured as RNA binding protein, RBM3 is involved in mRNA biogenesis as well as stimulating protein synthesis, promoting proliferation and exerting anti-apoptotic functions. Nowadays, accumulating immunohistochemically studies have suggested RBM3 function as a proto-oncogene that is associated with tumor progression and metastasis in various cancers. Moreover, emerging evidences have also indicated that RBM3 is equally effective in neuroprotection. In the present review, we provide an overview of current knowledge concerning the role of RBM3 in various cancers and neuroprotection. Additionally, its potential roles as a promising diagnostic marker for cancer and a possible therapeutic target for neuro-related diseases are discussed.

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Generation of mice deficient in RNA-binding motif protein 3 (RBM3) and characterization of its role in innate immune responses and cell growth

Cited by 28 publications

References 28 publications

Podocalyxin-like and RNA-binding motif protein 3 are prognostic biomarkers in urothelial bladder cancer: a validatory study

Podocalyxin-like and RNA-binding motif protein 3 are prognostic biomarkers in urothelial bladder cancer: a validatory study

TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM3

RNA binding motif protein 3: a potential biomarker in cancer and therapeutic target in neuroprotection

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