Generation of Medaka Fish Haploid Embryonic Stem Cells

Yi, Meisheng; Hóng, Ní; Hong, Yunhan

doi:10.1126/science.1175151

Cited by 164 publications

(249 citation statements)

References 25 publications

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“…3). In fish, the expression of boule, dazl and vasa has also been examined in medaka , a laboratory fish model for germ cell biology (Herpin et al, 2007;) and stem cell biology (Hong et al, 1996(Hong et al, , 1998Yi et al, 2009). Notably, the medaka dazl expression also exhibits differential intracellular distribution between male and female gonia (Fig.…”

Section: Resultsmentioning

confidence: 99%

Germ cell sex prior to meiosis in the rainbow trout

Li¹,

Shen²,

Wong³

et al. 2011

Protein Cell

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Germ cells make two major decisions when they move from an indeterminate state to their final stage of gamete production. One decision is sexual commitment for sperm or egg production, and the other is to maintain mitotic division or entry into meiosis. It is unclear whether the two decisions are made as a single event or separate events, because there has been no evidence for the presence of germ cell sex prior to meiosis. Here we report direct evidence in the fish rainbow trout that gonia have distinct sexuality. We show that dazl expression occurs in both male and female gonia but exhibits differential intracellular distribution. More strikingly, we show that boule is highly expressed in male gonia but absent in female gonia. Therefore, mitotic gonia possess sex, sperm/egg decision and mitosis/meiosis decision are two independent events, and sperm/egg decision precedes mitosis/meiosis decision in rainbow trout, making this organism a unique vertebrate model for mechanistic understanding of germ cell sex differentiation and relationship between the two decisions.

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Section: Resultsmentioning

confidence: 99%

Germ cell sex prior to meiosis in the rainbow trout

Li¹,

Shen²,

Wong³

et al. 2011

Protein Cell

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“…In fact, the production of germline chimeras from cell cultures in nonmammalian species represents a critical challenge towards the development of gene targeting technology. The lack of ES cell germline transmission in fish has led to alternative approaches such as somatic cell nuclear transfer [15,18], test-tube sperm production from testis-derived cell cultures [19], and semicloning by generating and utilizing haploid ES cells for direct nuclear transfer [20]. For germline chimera formation, an understanding of the accessibility and parameters for optimal contribution by ES cell cultures is required.…”

Section: Introductionmentioning

confidence: 99%

“…Differences in several aspects between ES cell cultures and host embryos appear to account for this low degree of chimerism. For example, the recipient blastula embryos of medaka divide every 35 min [21,22], whereas ES cell cultures such MES1 cells require more than 43 h to complete a cell cycle [9,20]. Furthermore, there is a limited input number of ES cell cultures permissive to normal development [21].…”

Section: Introductionmentioning

confidence: 99%

Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Hóng

Zeng

et al. 2010

Cell. Mol. Life Sci.

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Chimera formation is a powerful tool for analyzing pluripotency in vivo. It has been widely accepted that host cell lineages are generally accessible to embryonic stem (ES) cells with the actual contribution depending solely on the intrinsic pluripotency of transplanted donor cells. Here, we show in the fish medaka (Oryzias latipes) that the host accessibility to ES cell contribution exhibits dramatic differences. Specifically, of three albino host strains tested (i 1 , i 3 and af), only strain i 1 generated pigmented chimeras. Strikingly, this accessibility is completely lost in i 1 but acquired in i 3 after host c-irradiation. Host irradiation also differentially affected ES cell contribution to somatic organs and gonad. Therefore, the accessibility of various host cell lineages can vary considerably depending on host strains and cell lineages as well as on irradiation. Our findings underscore the importance of host genotypes for interpreting donor cell pluripotency and for improving ES-derived chimera production.

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“…Stem cell culture and application have been rapidly developed in model fishes of medaka and zebrafish [40,41], and some stem cell technologies, such as gene targeting, germ cell transplantation and semi-cloning by nuclear transfer, have been successfully formed because of specific potentials of fish germ stem cells and embryonic stem cells [42,43]. Especially, Yi et al [44] used the semi-cloning technology to create the semi-cloned female medaka which shows normal fertility and germline transmission over three generations. This approach represents a significant development for germline transmission of cultured cells, and will be a potential reproduction technology to combat infertility in vertebrates including human.…”

Section: Somatic Cell Nuclear Transfer and Stem Cell Technologies In mentioning

confidence: 99%

Molecular basis and genetic improvement of economically important traits in aquaculture animals

2012

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Aquaculture has been believed to be a major Chinese contribution to the world. In recent 20 years, genome and other genetic technologies have promoted significant advances in basic studies on molecular basis and genetic improvement of aquaculture animals, and complete genomes of some main aquaculture animals have been sequenced or announced to be sequenced since the beginning of this century. Here, we review some significant breakthrough progress of aquaculture genetic improvement technologies including genome technologies, somatic cell nuclear transfer and stem cell technologies, outline the molecular basis of several economically important traits including reproduction, sex, growth, disease resistance, cold tolerance and hypoxia tolerance, and present a series of candidate trait-related genes. Finally, some application cases of genetic improvement are introduced in aquaculture animals, especially in China, and several development trends are highlighted in the near future.

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Generation of Medaka Fish Haploid Embryonic Stem Cells

Cited by 164 publications

References 25 publications

Germ cell sex prior to meiosis in the rainbow trout

Germ cell sex prior to meiosis in the rainbow trout

Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Molecular basis and genetic improvement of economically important traits in aquaculture animals

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