Generation of magnetic nonviral gene transfer agents and magnetofection in vitro

Mykhaylyk, Olga; Antequera, Yolanda Sánchez; Vlaskou, Dialekti; Plank, Christian

doi:10.1038/nprot.2007.352

Cited by 259 publications

(242 citation statements)

References 44 publications

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“…For example, Plank and co-workers synthesized magnetic particles in situ in PEI solutions [8,15,16], Hottiger and coworkers directly mixed maghemite dispersions with PEI solutions [10], whilst McBain and co-workers exploited covalent bonds to attach PEI to magnetic particles [17]. The MP-PEI prepared by these methods are all capable of enhancing gene delivery.…”

Section: Nano Researchmentioning

confidence: 99%

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

et al. 2009

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Using magnetic nanoparticles to enhance gene transfection, a recently developed technique termed magnetofection, has been shown to be a powerful technology in gene delivery. The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine, which is a well known nonviral transfection agent. In this article, we report methods to control the aggregate size of polyethyleneiminecoated magnetite particles. These particles were then used to enhance transfection of green fl uorescent protein (GFP) into NIH 3T3 cells in vitro. We find that the aggregate size of the particles has a great effect on their performance in magnetofection, with less aggregated magnetic particles being more effective in enhancing the gene transfection.

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Section: Nano Researchmentioning

confidence: 99%

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

et al. 2009

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“…IONPs stabilized with cationic polymers can, however, be used to deliver DNA or RNA [38]. Transfection using these IONPs can be improved using a magnetic fi eld (magnetofection) [39].…”

Section: Polymer Propertiesmentioning

confidence: 99%

The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications

et al. 2010

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Iron-oxide nanoparticles (IONPs) represent a significant class of inorganic nanomaterial that is contributing to the current revolution in nanomedicine [1][2]. Their unique physical properties, including high surface area to volume ratios and superparamagnetism, confer useful attributes for medical applications such as magnetic resonance imaging (MRI), drug and gene delivery, tissue engineering and bioseparation [3].Two distinct classes of superparamagnetic IONP-based materials are currently used for medical applications: superparamagnetic iron-oxide (SPIO) nanoparticles with a mean particle diameter of 50-100 nm, and ultra-small superparamagnetic iron-oxide (USPIO) nanoparticles with a size below 50 nm. Th ese two classes of IONPs have been studied widely for medical applications, particularly as the next (potential) generation of MRI contrast agents. Th ey are also seen as potential vectors for drug and gene delivery. Th e biodistribution of these nanoparticles can be altered by the application of an external magnetic fi eld; they also have potential applications in hyperthermia therapy as some magnetic particles can heat up under the infl uence of a localized high-frequency magnetic fi eld.Th e intent of this review is to present recent advances in the synthesis of IONPs and their subsequent stabilization in biological fluids using polymers, focusing on the current strategies used to graft polymers onto IONPs surfaces (see Figure 1) and the diff erent types of polymers used. Th e additional properties conferred by the polymers, such as targeting, eff ects on biodistribution and pharmacokinetics, are also discussed, and the main applications of IONPs are reviewed. Synthesis and properties of iron-oxide nanoparticlesSPIO and USPIO nanoparticles are the most extensively studied magnetic nanoparticles for biomedical applications as they are both biocompatible and easy to synthesize. Both are composed of ferrite nanocrystallites of magnetite (Fe 3 O 4 ) or maghemite (Fe 2 O 3 , γ). Over the last ten years, there has been an explosion of interest in these materials and this has been reflected in a large number of recent publications describing the synthesis and modifi cation of hybrid IONPs. In this review, we focus on the polymer modifi cation of the nanoparticles, and provide only minimal information on the inorganic synthesis of IONPs. For more detailed information on IONP synthesis, readers are referred to other reviews [3,4]. The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applicationsCyrille Boyer 1 * , Michael R. Whittaker 1 , Volga Bulmus 2 , Jingquan Liu 1 and Thomas P. Davis 1 * University of New South Wales, AustraliaOver the past decade, the synthesis of superparamagnetic nanoparticles, especially iron-oxide nanoparticles (IONPs), has been researched intensively for many high-technology applications, including enhanced storage media, biosensing and medical applications. In medicine, IONPs are used as contrast agents in magnetic resonance imaging and in hyperthermia...

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“…[165] Magnetofection has been successfully applied to a wide range of primary cells and cells that are hard to transfect using other nonviral methods. [166] Recent work using a local injection of the nanoparticles into the gastrointestinal track and the ear vasculature [167] imply that this well accepted method for in vitro gene delivery may be applicable to in vivo gene delivery.…”

Section: Magnetofectionmentioning

confidence: 99%

Advances in Gene Delivery Systems

et al. 2011

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The transfer of genes into cells, both in vitro and in vivo, is critical for studying gene function and conducting gene therapy. Methods that utilize viral and nonviral vectors, as well as physical approaches, have been explored. Viral vector-mediated gene transfer employs replication-deficient viruses such as retro-virus, adenovirus, adeno-associated virus and herpes simplex virus. A major advantage of viral vectors is their high gene delivery efficiency. The nonviral vectors developed so far include cationic liposomes, cationic polymers, synthetic peptides and naturally occurring compounds. These nonviral vectors appear to be highly effective in gene delivery to cultured cells in vitro but are significantly less effective in vivo. Physical methods utilize mechanical pressure, electric shock or hydrodynamic force to transiently permeate the cell membrane to transfer DNA into target cells. They are simpler than viral-and nonviral-based systems and highly effective for localized gene delivery. The past decade has seen significant efforts to establish the most desirable method for safe, effective and target-specific gene delivery, and good progress has been made. The objectives of this review are to (i) explain the rationale for the design of viral, nonviral and physical methods for gene delivery; (ii) provide a summary on recent advances in gene transfer technology; (iii) discuss advantages and disadvantages of each of the most commonly used gene delivery methods; and (iv) provide future perspectives.

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Generation of magnetic nonviral gene transfer agents and magnetofection in vitro

Cited by 259 publications

References 44 publications

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

Control of aggregate size of polyethyleneimine-coated magnetic nanoparticles for magnetofection

The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications

Advances in Gene Delivery Systems

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