Generation of light sheet focal patterns for light-sheet fluorescence microscopy via phase modulated radial and azimuthal variant vector beam

Abstract

Axial multiple focal patterns are theoretically generated by tight focusing of radial and azimuthal variant vector beam phase modulated by annular Walsh filter. The novel light sheet patterns with sub wavelength size such as longitudinally polarized multiple focal spots, transversely polarized multiple focal spots/holes, longitudinally/ transversely polarized annular dark multiple spots with petal shapes are numerically generated by properly manipulating the parameters such as radial & azimuthal index values as well as the order & size of the annular obstruction values. The pre mentioned novel multiple focal patterns highly applicable in axial multiple optical trapping and manipulating, light-sheet fluorescence microscopy and optical lithography etc.