2017
DOI: 10.1016/j.mce.2017.02.030
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Generation of kisspeptin-responsive GnRH neurons from human pluripotent stem cells

Abstract: GnRH neurons are fundamental for reproduction in all vertebrates, integrating all reproductive inputs. The inaccessibility of human GnRH-neurons has been a major impediment to studying the central control of reproduction and its disorders. Here, we report the efficient generation of kisspeptin responsive GnRH-secreting neurons by directed differentiation of human Embryonic Stem Cells and induced-Pluripotent Stem Cells derived from a Kallman Syndrome patient and a healthy family member. The protocol involves th… Show more

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Cited by 16 publications
(11 citation statements)
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References 45 publications
(60 reference statements)
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“…Further studies are warranted to define the exact mechanism of FGF21 action on neuroglial–endothelial plasticity in vivo and its physiological relevance for the GnRH system. Induced pluripotent stem cell (iPSC)‐derived GnRH neurons (Lund et al , ; Poliandri et al , ) from CHH patients with KLB mutations would be an excellent model to elucidate how the human KLB mutants affect GnRH secretion.…”
Section: Discussionmentioning
confidence: 99%
“…Further studies are warranted to define the exact mechanism of FGF21 action on neuroglial–endothelial plasticity in vivo and its physiological relevance for the GnRH system. Induced pluripotent stem cell (iPSC)‐derived GnRH neurons (Lund et al , ; Poliandri et al , ) from CHH patients with KLB mutations would be an excellent model to elucidate how the human KLB mutants affect GnRH secretion.…”
Section: Discussionmentioning
confidence: 99%
“…Primary cultures of USCs, fibroblasts, and L-EPCs were isolated as previously described ( Martin-Ramirez et al., 2012 , Poliandri et al., 2017 , Zhou et al., 2012 ). BMECs were a kind gift of Dr. Egle Solito ( Schweitzer et al., 1997 , Solito et al., 2000 ), and HEK293T lines were obtained from ATCC.…”
Section: Methodsmentioning
confidence: 99%
“…Cell Culture Primary cultures of USCs, fibroblasts, and L-EPCs were isolated as previously described (Martin-Ramirez et al, 2012;Poliandri et al, 2017;Zhou et al, 2012). BMECs were a kind gift of Dr. Egle Solito (Schweitzer et al, 1997;Solito et al, 2000), and HEK293T lines were obtained from ATCC.…”
Section: Methodsmentioning
confidence: 99%