2006
DOI: 10.1128/jvi.80.9.4633-4639.2006
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Generation of Infectious Hepatitis C Virus in Immortalized Human Hepatocytes

Abstract: Progress in understanding hepatitis C virus (HCV) biology has remained a challenge due to the lack of an efficient cell culture system for virus growth. In this study, we examined HCV core protein-mediated immortalized human hepatocytes (

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Cited by 85 publications
(113 citation statements)
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References 33 publications
(41 reference statements)
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“…Expression of HCV core protein as well as HCV full-length protein leads to activation of the AR signaling pathway in the presence of androgen [43]. Cell culture-grown HCV infection in human hepatocytes also augments AR-mediated signaling in the presence of androgen [43,44]. These results showed that HCV core protein might cross-talk with the AR signaling pathway for promotion of carcinogenesis.…”
Section: Androgen Receptor-mediating Signaling In Hcvrelated Hepatocamentioning
confidence: 57%
“…Expression of HCV core protein as well as HCV full-length protein leads to activation of the AR signaling pathway in the presence of androgen [43]. Cell culture-grown HCV infection in human hepatocytes also augments AR-mediated signaling in the presence of androgen [43,44]. These results showed that HCV core protein might cross-talk with the AR signaling pathway for promotion of carcinogenesis.…”
Section: Androgen Receptor-mediating Signaling In Hcvrelated Hepatocamentioning
confidence: 57%
“…2 and 3), a distinct advantage of our assay over other assays using surrogate viruses. A cell culture system for the growth of HCV has also been developed using unique clones derived from HCV strains (15,16). The genetic basis for the propagation of these specific clones and the lack of propagation of most other HCVs in culture are not understood in detail.…”
Section: Discussionmentioning
confidence: 99%
“…Recently, several assay systems, such as recombinant HCV envelope glycoproteins (13,14), HCV pseudotyped particles (4,5), and cell culture-derived infectious HCV particles (15,16), have been developed to study HCV-host cell interactions. These systems have allowed the identification of cell surface molecules, such as CD81 (13), scavenger receptor class B type I (14), claudin-1 (17), occludin (18), dendritic cell/liver-specific ICAM-3 grabbing nonintegrin (19), low density lipoprotein receptor (20), and heparan sulfate (21), that are involved in the mechanism of HCV entry into cells.…”
Section: Hepatitis C Virus (Hcv) Belongs To the Genusmentioning
confidence: 99%
“…However, the production of HCV particles in these cells appears to be restricted to a short period not exceeding a few days. Interestingly, recent studies demonstrated that HCV infection triggers in these cells both IFN signaling pathway and an autophagic response (Ait-Goughoulte et al, 2008;Kanda et al, 2006;Kanda et al, 2007). (Carloni et al, 1993;Iacovacci et al, 1997).…”
Section: Human Hepatocarcinoma and Immortalized Hepatocyte (Ihh)-basementioning
confidence: 99%