Generation of hydroxyl radical from linoleic acid hydroperoxide in the presence of epinephrine and iron

Angiogenic Effect of Lipid Hydroperoxide on Bovine Aortic Endothelial Cells.

“…Lipid peroxy radicals are formed by the decomposition of LHP in the presence of ferric iron or cupric iron [25]. Propagation reactions are a unique feature of LHP and thereby produce a constant supply of radical species [26][27][28]. Thus, scavenging of these radicals would be predicted to have an inhibitory effect on angiogenesis.…”

Protective Effect of Catecholestrogen on Lipid Hydroperoxide-Induced Injury to Cultured Bovine Aortic Endothelial Cells and on Lipid Peroxidation of Low-Density Lipoprotein.

“…E2 was purchased from Nacalai tesque Inc., Kyoto, Japan; and 2-OHE2, from Sigma Chemical Co., St. Louis, MO. Linoleic acid hydroperoxide was prepared by incubation of linoleic acid with lipoxygenase as described by Ohkawa et al [22] and purified by high-performance thin-layer chromatography as described previously [23].…”

Formation of Superoxide Anion during Ferrous Ion-Induced Decomposition of Linoleic Acid Hydroperoxide under Aerobic Conditions

“…were not contributors to the emission. Judging from the time-dependent change in the LOOH concentration [as monitored by HPLC-CL (18)], the Fe 2+ -catalyzed decomposition of LOOH in potassium phosphate buffer stopped within an extremely short time (data not shown), probably because Fe 2+ was chelated by phosphate and/or converted to ferric hydroxide [Fe(OH) 3 ], which is poorly soluble in water (1). The addition of DMPO (12 mM) had no effect on the chemiluminescence intensity (data not shown).…”