Generation of human umbilical cord vein CD146+ perivascular cell origined three-dimensional vascular construct

Perivascular Mesenchymal Progenitors for Bone Regeneration

“…Supporting a contact‐independent role for induction of vascularization, PSC transcribe and secrete greater amounts of pro‐vasculogenic growth factors including fibroblast growth factor 2 (FGF2) and vascular endothelial growth factor (VEGF) among others. In addition, other investigators have found analogous pro‐vasculogenic effects among CD146 + pericytes or PSC in non‐orthopaedic tissue engineering efforts . In sum, perivascular progenitors, like other mesenchymal progenitor cell preparations, exert a combination of pro‐osteogenic and pro‐vasculogenic effects that result in vascularized ectopic bone formation (Fig.…”

“…In addition, other investigators have found analogous provasculogenic effects among CD146 + pericytes or PSC in non-orthopaedic tissue engineering efforts. [20][21][22][23] In sum, perivascular progenitors, like other mesenchymal progenitor cell preparations, exert a combination of proosteogenic and pro-vasculogenic effects that result in vascularized ectopic bone formation ( Fig. 1).…”

Nonlinear Elasticity of Blood Vessels and Vascular Grafts

“…82 Gokcinar-Yagci et al developed a cell sheet vascular graft that closely mimicked the three-layer structure of blood vessels, although the mechanical properties of their product were not explicitly demonstrated. 83 Xu W et al summarized the patterned cell sheet technology, which enables control over the structure of cell sheets through the morphology of the cell growth substrate, leading to the fabrication of vascular grafts with nonlinear elasticity and anisotropic mechanical behaviors (Figure 5A, B). 84−87 To fabricate bioactive grafts with nonlinear elasticity, the application of bioreactors is crucial for replicating the microenvironment of the native extracellular matrix and preserving the physiological mechanical properties (Figure 5C, D).…”

“…L’Heureux et al successfully prepared a vascular graft composed of pure human cell-derived sheets that fulfilled the mechanical requirements for vascular grafting . Gökçinar-Yagci et al developed a cell sheet vascular graft that closely mimicked the three-layer structure of blood vessels, although the mechanical properties of their product were not explicitly demonstrated . Xu W et al summarized the patterned cell sheet technology, which enables control over the structure of cell sheets through the morphology of the cell growth substrate, leading to the fabrication of vascular grafts with nonlinear elasticity and anisotropic mechanical behaviors (Figure A, B). − …”

Applications of extraembryonic tissue-derived cells in vascular tissue regeneration

“…Collagen type I/elastin/dermatan sulfate was used to form the tunica media, and collagen type I/fibrin was used for the tunica adventitia. Subsequently, HUVECs were seeded onto the construct using the cell sheet engineering method [ 88 ].…”