Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a modified RNA system

Abstract

We generated human induced pluripotent stem cells (KSCBi002-B and KSCBi002-B-1) from the dermal fibroblasts of a donor using a modified RNA-based gene delivery method. According to GTG-banding analysis, the generated KSCBi002-B line has a cytogenetic abnormality (46,XY, t(1;4)(q21;q25)) that is distinct from that of the donor, whereas KSCBi002-B-1 has a normal karyotype (46,XY). These cell lines can be useful as a model for characterizing the hiPSCs generated by a non-viral and non-integrative system, or as a chromosomal balanced translocation model. These two cell lines are registered and available from the National Stem Cell Bank, Korea National Institute of Health.

Functional in vivo and in vitro effects of 20q11.21 genetic aberrations on hPSC differentiation

“…Supplementary Fig. S1 a and Table S1 present detailed sample information, and the cell lines have been characterized in previous reports 22 – 24 .…”

“…Each cell line was studied over an extended culture period. The hiPSCs were reprogrammed from primary cells as described previously 22 – 24 . The hiPSC lines were derived from two types of primary cells, fibroblasts and urine cells, and reprogramming was induced with Sendai virus and modified mRNA.…”