Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a non-integration system

Uhm, Kyung-Ok; Kim, Sojung; Jo, Eun Hee; Go, Gue Youn; Choi, Hye Young; Im, Young Sam; Ha, Hye-Yeong; Kim, Jung-Hyun; Koo, Soo Kyung

doi:10.1016/j.scr.2017.03.009

Cited by 7 publications

(4 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supplementary Fig. S1 a and Table S1 present detailed sample information, and the cell lines have been characterized in previous reports 22 – 24 .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Functional in vivo and in vitro effects of 20q11.21 genetic aberrations on hPSC differentiation

Lee

Ahn

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Human pluripotent stem cells (hPSCs) have promising therapeutic applications due to their infinite capacity for self-renewal and pluripotency. Genomic stability is imperative for the clinical use of hPSCs; however, copy number variation (CNV), especially recurrent CNV at 20q11.21, may contribute genomic instability of hPSCs. Furthermore, the effects of CNVs in hPSCs at the whole-transcriptome scale are poorly understood. This study aimed to examine the functional in vivo and in vitro effects of frequently detected CNVs at 20q11.21 during early-stage differentiation of hPSCs. Comprehensive transcriptome profiling of abnormal hPSCs revealed that the differential gene expression patterns had a negative effect on differentiation potential. Transcriptional heterogeneity identified by single-cell RNA sequencing (scRNA-seq) of embryoid bodies from two different isogenic lines of hPSCs revealed alterations in differentiated cell distributions compared with that of normal cells. RNA-seq analysis of 22 teratomas identified several differentially expressed lineage-specific markers in hPSCs with CNVs, consistent with the histological results of the altered ecto/meso/endodermal ratio due to CNVs. Our results suggest that CNV amplification contributes to cell proliferation, apoptosis, and cell fate specification. This work shows the functional consequences of recurrent genetic abnormalities and thereby provides evidence to support the development of cell-based applications.

show abstract

“…Supplementary Fig. S1 a and Table S1 present detailed sample information, and the cell lines have been characterized in previous reports 22 – 24 .…”

Section: Resultsmentioning

confidence: 99%

“…Each cell line was studied over an extended culture period. The hiPSCs were reprogrammed from primary cells as described previously 22 – 24 . The hiPSC lines were derived from two types of primary cells, fibroblasts and urine cells, and reprogramming was induced with Sendai virus and modified mRNA.…”

Section: Methodsmentioning

confidence: 99%

Functional in vivo and in vitro effects of 20q11.21 genetic aberrations on hPSC differentiation

Lee

Ahn

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…EDTA was added for 3 min. The human induced pluripotent stem cell line, hFSiPS1 (KSCBi002-A), and the human embryonic stem cell line, SNUhES31 (SNUe007-A, established by Seoul National University), used in this study, were obtained from the Korea National Stem Cell Bank 77 , 78 .…”

Section: Methodsmentioning

confidence: 99%

DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociation

Yoo

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Cultured human pluripotent stem cells (hPSCs) grow as colonies that require breakdown into small clumps for further propagation. Although cell death mechanism by single-cell dissociation of hPSCs has been well defined, how hPSCs respond to the deadly stimulus and recover the original status remains unclear. Here we show that dissociation of hPSCs immediately activates ERK, which subsequently activates RSK and induces DUSP6, an ERK-specific phosphatase. Although the activation is transient, DUSP6 expression persists days after passaging. DUSP6 depletion using the CRISPR/Cas9 system reveals that DUSP6 suppresses the ERK activity over the long term. Elevated ERK activity by DUSP6 depletion increases both viability of hPSCs after single-cell dissociation and differentiation propensity towards mesoderm and endoderm lineages. These findings provide new insights into how hPSCs respond to dissociation in order to maintain pluripotency.

show abstract

“…The human iPSCs line hFSiPS1, which was derived from male dermal fibroblasts, 53 was acquired from the National Stem Cell Bank of Korea. The iPSCs were routinely maintained for 5–6 days on a dish coated with Matrigel (Corning) using Essential 8 (E8) feeder‐free medium (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

Therapeutic potential of mesenchymal stem cells from human iPSC‐derived teratomas for osteochondral defect regeneration

Kim,

Kim

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

Human induced pluripotent stem cells (iPSCs) hold great promise for personalized medicine, as they can be differentiated into specific cell types, especially mesenchymal stem cells (MSCs). Therefore, our study sought to assess the feasibility of deriving MSCs from teratomas generated from human iPSCs. Teratomas serve as a model to mimic multilineage human development, thus enriching specific somatic progenitors and stem cells. Here, we discovered a small, condensed mass of MSCs within iPSC‐generated teratomas. Afterward, we successfully isolated MSCs from this condensed mass, which was a byproduct of teratoma development. To evaluate the characteristics and cell behaviors of iPSC‐derived MSCs (iPSC‐MSCs), we conducted comprehensive assessments using qPCR, immunophenotype analysis, and cell proliferation‐related assays. Remarkably, iPSC‐MSCs exhibited an immunophenotype resembling that of conventional MSCs, and they displayed robust proliferative capabilities, similar to those of higher pluripotent stem cell‐derived MSCs. Furthermore, iPSC‐MSCs demonstrated the ability to differentiate into multiple lineages in vitro. Finally, we evaluated the therapeutic potential of iPSC‐MSCs using an osteochondral defect model. Our findings demonstrated that teratomas are a promising source for the isolation of condensed MSCs. More importantly, our results suggest that iPSC‐MSCs derived from teratomas possess the capacity for tissue regeneration, highlighting their promise for future therapeutic applications.

show abstract

Generation of human induced pluripotent stem cell lines from human dermal fibroblasts using a non-integration system

Cited by 7 publications

References 1 publication

Functional in vivo and in vitro effects of 20q11.21 genetic aberrations on hPSC differentiation

Functional in vivo and in vitro effects of 20q11.21 genetic aberrations on hPSC differentiation

DUSP6 is a memory retention feedback regulator of ERK signaling for cellular resilience of human pluripotent stem cells in response to dissociation

Therapeutic potential of mesenchymal stem cells from human iPSC‐derived teratomas for osteochondral defect regeneration

Contact Info

Product

Resources

About