Generation of human induced pluripotent stem cells from oral mucosa

Strategies for Oral Mucosal Repair by Engineering 3D Tissues with Pluripotent Stem Cells

“…Dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, as well as oral mucosal fibroblasts, have all shown a high efficiency of reprogrammed to iPSC. 13,14 Oral hard and soft tissues may prove to be a very useful source of cells for reprogramming, as oral biopsies and extracted teeth are easily accessible for cell isolation and expansion.…”

Induced Pluripotent Stem Cells and Their Implication for Biomedical Sciences

“…The mostly use somatic cells are fibroblasts, but different groups generated also iPS cells from other somatic cells providing evidence that is possible to reprogram cells of different origins (Deng, 2010;Ebben et al, 2011;Patel and Yang, 2010;Uemura et al, 2012;Vitale et al, 2011;Walia et al, 2012;Wong and Chiu, 2011;Zeng and Zhou, 2011). Other sources of iPSCs that can be easily reprogrammed are human keratinocytes (Aasent et al, 2008;Petit et al, 2012), oral mucosa fibroblasts (Miyoshi et al, 2010), dermal papilla cells (Tsai et al, 2010), pancreatic beta cells (Stadtfeld et al, 2008), neural stem cells (Kim et al, 2009), mature B lymphocytes (Hanna et al, 2008), liver and stomach cells (Aoi et al, 2008) and cord blood cells (Cai et al, 2010;Takenaka et al, 2009). For example, human keratinocytes have been shown to be 100-fold more efficient and 2-fold faster than human fibroblasts (Aasent et al, 2008).…”

Therapeutic Approaches in Regenerative Medicine of Cardiovascular Diseases: From Bench to Bedside

“…It allows patient-specific stem cells to be obtained by using the same genome as that of the individual whose cells have been reprogrammed. Besides adult and foetal dermal fibroblasts, iPSCs have been created from multiple human tissues, including lung fibroblasts, keratinocytes (Aasen et al, 2008), fibroblast-like synoviocytes (Takahashi et al, 2007), cord blood (Giorgetti et al, 2009;Haase et al,2009) , peripheral blood (Loh et al, 2009, Ye et al, 2009) mesenchymal stromal cells , oral mucosa fibroblasts (Miyoshi et al, 2010) and T-cells (Loh et al, 2010;Seki et al, 2010). These cells can be differentiated and can serve as a cell or disease model or may even lead to future stem cell therapies (Dimos et al, 2008, Park et al, 2008.…”