Generation of human and rabbit recombinant antibodies for the detection of Zearalenone by phage display antibody technology

Anti-Staphylococcus aureus Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo

“…At present, the use of phage libraries expressing mouse and human antibody fragments almost became routine in the respective field of biomedical research. Over the past few years, the application of phage display technology in livestock research has increased (e.g., phage display single chain antibody library generated from camels [24], rabbit [25], pig [26], sheep [27], and chicken [28]). However, only a few studies obtained scFvs by phage surface display technology and proved the protective effects of scFvs on bovine mastitis and piglet epizootic diarrhea.…”

Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume

“…Phage display is a powerful technology for the generation of human monoclonal antibodies ( 13 ). The scFv format has several advantages over other antibody formats, namely, more efficient and stable for display on phage coats ( 23 ), higher permeability due to nano size ( 24 ), easy to express in E. coli ( 25 ) and other expression hosts ( 26 ). It can also be further engineered to suit various assay formats, including biosensor-based detection ( 15 ).…”

“…The antibody library in this study was created from a healthy population that had previously encountered a myriad of antigens in the region of northeastern Thailand around 15 years ago, apparently from this study, including nitrogen-fixing soil bacteria. Specific scFv antibodies from this library has been generated against a wide variety of targets such as mycotoxins ( 15 , 25 ), venom ( 28 ), pathogenic bacteria ( 17 ), as well as virus ( 29 ) and cancer biomarker ( 30 ). From our previous study, the recombinant scFv antibody that could bind specifically to Bradyrhizobium strain DOA9 was successfully generated ( 18 ), from a phage-displayed rabbit scFv antibody library, created from an immunized rabbit.…”

Recombinant antibodies and their use for food immunoanalysis

“…Several studies have shown that the use of alkaline phosphatase (AP) fusion proteins (scFv-AP, V HH -AP) in replacement of free scFv or V HH fragments usually results in competitive ELISAs with better detection limits, shorter analysis times, and reduced variability, due to the elimination of one assay step when compared with an indirect ELISA [75,84]. This effect is attributed to an avidity effect since AP exists as a dimer in solution; therefore, the antibody fragments would also bind effectively as dimers [40].…”