Generation of hircine-induced pluripotent stem cells by somatic cell reprogramming

Generation of Arbas Cashmere Goat Induced Pluripotent Stem Cells Through Fibroblast Reprogramming

“…However, exogenous gene expression decreased in the absence of Dox; the giPSCs could not maintain their original morphology, and the cells differentiated rapidly. This proves that the existing culture environment was insufficient for maintaining the pluripotency of the giPSCs we established under no-Dox conditions (Bao et al, 2011;Li et al, 2011;Ren et al, 2011).…”

“…At present, the establishment of goat stable ESC lines has not been reported. In 2011, Ren et al (2011) successfully established giPSCs. However, they transferred six genes, reducing the direct application potential of the cell lines, and the stem cell purity of the giPSCs has been questioned.…”

“…Therefore, iPSCs are another possible means of obtaining goat ESCs (Takahashi and Yamanaka, 2006). Ren et al (2011) reported that goat iPSCs (giPSCs) obtained following the transfer of OCT4, SOX2, c-MYC, KLF4, SV40LT, and hTERT into goat primary ear fibroblasts and reprogramming had similar morphology and cell characteristics to mouse ESCs. The authors were unable to generate stable giPSCs using the classic OSMK combination (OCT3/4, SOX2, c-MYC, KLF4).…”

A poor imitation of a natural process

“…The use of such assays leads to the identification of false positives and the incorrect evaluation as human, 47 sheep 48 and goat. 49 However, these factors are potent viral oncoproteins that can inactivate the p53 and the retinoblastoma pathways. 50 (2) The non-specific and broad action of the chemicals may induce the dysregulation of gene expression.…”

A special issue on cell signaling, disease, and stem cells

“…We chose to highlight these stem cell papers in this special issue as stem cell research is intrinsically connected to the study of cell signaling and disease. The collection of a large number of stem cell papers also reflects the fact that stem cell research has become an important part of the growing content of Cell Research, as highlighted by a large number of important discoveries published in the journal in the past year alone, such as the successful generation of iPS cells with a single factor Oct4 [3,4], efficient generation of human iPS cells from blood cells via a non-integrating method [5], generation of functional platelets from hESCs [6], directed differentiation of atrial and ventricular myocytes from hESCs [7], the successful generation of ovine, hircine and bovine iPS cells [8][9][10], somatic cell reprogramming using electrofused blastomeres [11], improved methods for iPS cell generation [12,13], gener-ating iPS cells using Bmi1 [14], comprehensive analyses of chromatin state dynamics in hESC differentiation [15], characterization of neuroblasts in the adult human brain [16], identification of lectin biomarkers for human pluripotent stem cell isolation [17], and efficient correction of disease-causing genetic mutations in patient iPS cells [18].…”