Generation of high quality of hepatocyte-like cells from induced pluripotent stem cells with Parp1 but lacking c-Myc

Huang, Cheng-Yang; Lin, Hsin-Chi; Lu, Kai-Hsi; Wu, Wai-Wah; Yang, Ya-Chi; Yang, Yi‐Ping; Chiang, Chih‐Hung; Hsieh, Jung-Hung; Chang, Yuh‐Lih; Lee, Shou‐Dong

doi:10.1016/j.jcma.2018.06.002

Cited by 13 publications

(12 citation statements)

References 31 publications

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“…PARP1 was able to facilitate the reprogramming of senescent fibroblasts and the resultant iPSCs were differentiated into hepatocytes. [55][56][57] Combinatorial use of these molecules and transcription factors can synergistically enhance the efficiency of reprogramming and iPSC generation.…”

Section: Small Molecule Modulatorsmentioning

confidence: 99%

“…Some studies have reported the use of poly (ADP ribose) polymerase 1 (PARP 1) which catalyses ADP ribosylation, as a reprogramming factor replacing c‐Myc. PARP1 was able to facilitate the reprogramming of senescent fibroblasts and the resultant iPSCs were differentiated into hepatocytes 55–57 . Combinatorial use of these molecules and transcription factors can synergistically enhance the efficiency of reprogramming and iPSC generation.…”

Section: Reprogramming Factorsmentioning

confidence: 99%

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Recent advances in the induced pluripotent stem cell‐based skin regeneration

Choudhury

Surendran

Das

2021

Wound Repair Regeneration

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Skin regeneration has been a challenging clinical problem especially in cases of chronic wounds such as diabetic foot ulcers, and epidermolysis bullosa-related skin blisters. Prolonged non-healing wounds often lead to bacterial infections increasing the severity of wounds. Current treatment strategies for chronic wounds include debridement of wounds along with antibiotics, growth factors, and stem cell transplantation therapies. However, the compromised nature of autologous stem cells in patients with comorbidities such as diabetes limits the efficacy of the therapy. The discovery of induced pluripotent stem cell (iPSC) technology has immensely influenced the field of regenerative therapy. Enormous efforts have been made to develop integration-free iPSCs suitable for clinical therapies. This review focuses on recent advances in the methods and reprogramming factors for generating iPSCs along with the existing challenges such as genetic alterations, tumorigenicity, immune rejection, and regulatory hurdles for the clinical application of iPSCs. Furthermore, this review also highlights the benefits of using iPSCs for the generation of skin cells and skin disease modeling over the existing clinical therapies for skin regeneration in chronic wounds and skin diseases.

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Section: Small Molecule Modulatorsmentioning

confidence: 99%

Section: Reprogramming Factorsmentioning

confidence: 99%

Recent advances in the induced pluripotent stem cell‐based skin regeneration

Choudhury

Surendran

Das

2021

Wound Repair Regeneration

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“…This development is the result of the careful selective expression of transcription factors together. Hepatocyte differentiation is recognized to be coordinately controlled by the act of multiple hepatocyte nuclear transcription factors, for example, hepatocyte nuclear factor (HNF)-1α and -1β; HNF-3α, -β, and -γ; HNF-4α, HNF-6; and CCAAT/enhancer-binding protein α and β [213]. A liverenriched transcription factor, HNF-3β, prompts the primary development of the endoderm, the predecessor to produce the liver.…”

Section: Genetic Manipulation In Dictating Hepatic-like Featuresmentioning

confidence: 99%

“…A liverenriched transcription factor, HNF-3β, prompts the primary development of the endoderm, the predecessor to produce the liver. HNF-3β in the developing endoderm is essential for the expression of HNF-4α, which shows a potential action in hepatocyte differentiation [213,214]. Recently, HNF-3β Overexpression has been presented to prompt endoderm differentiation of ES cells.…”

Section: Genetic Manipulation In Dictating Hepatic-like Featuresmentioning

confidence: 99%

“…Huang et al demonstrated that Parp1 can improve iPSC generation from somatic cells in the existence of three other reprogramming factors Oct4/ Sox2/Klf4 and after stimulation of hepatic differentiation into hepatocyte-like cells. Oct4/Sox2/Klf4-iPSC-Heps expressed liver-specific markers and characteristics, exhibited mature hepatocyte roles [213].…”

Section: Genetic Manipulation In Dictating Hepatic-like Featuresmentioning

confidence: 99%

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Current progress in hepatic tissue regeneration by tissue engineering

et al. 2019

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Background: Liver, as a vital organ, is responsible for a wide range of biological functions to maintain homeostasis and any type of damages to hepatic tissue contributes to disease progression and death. Viral infection, trauma, carcinoma, alcohol misuse and inborn errors of metabolism are common causes of liver diseases are a severe known reason for leading to end-stage liver disease or liver failure. In either way, liver transplantation is the only treatment option which is, however, hampered by the increasing scarcity of organ donor. Over the past years, considerable efforts have been directed toward liver regeneration aiming at developing new approaches and methodologies to enhance the transplantation process. These approaches include producing decellularized scaffolds from the liver organ, 3D bio-printing system, and nano-based 3D scaffolds to simulate the native liver microenvironment. The application of small molecules and micro-RNAs and genetic manipulation in favor of hepatic differentiation of distinct stem cells could also be exploited. All of these strategies will help to facilitate the application of stem cells in human medicine. This article reviews the most recent strategies to generate a high amount of mature hepatocyte-like cells and updates current knowledge on liver regenerative medicine.

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Crosstalk of moderate ROS and PARP‐1 contributes to sustainable proliferation of conditionally reprogrammed keratinocytes

Liu

Mondal

Liu

2022

J Biochem & Molecular Tox

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Conditionally reprogrammed cell (CRC) technique is a promising model for biomedical and toxicological research. In the present study, our data first demonstrated an increased level of PARP-1 in conditionally reprogrammed human foreskin keratinocytes (CR-HFKs). We then found that PARP inhibitor ABT-888 (ABT), reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine (NAC), or combination (ABT + NAC) were able to inhibit cell proliferation, ROS, PARP-1, and ROS related protein, NRF2, and NOX1. Interestingly, knockdown of endogenous PARP-1 significantly inhibited cell proliferation, indicating that the increased PARP-1 expression was critical for CR. Importantly, we found that a moderate level of ROS contributed the cell proliferation and increased PARP-1 since knockdown of PARP-1 also inhibited the ROS. The similar inhibition of cell proliferation, ROS, and expression of PARP-1 and NRF2 proteins was observed when CR-HFKs were treated with hydroquinone (HQ), a key component from skin-lightening products.Moreover, the treatment of HQ plus treatment of ABT, NAC, or combination can further inhibit cell proliferation, ROS, expression of PARP-1, and NRF2 proteins.PARP-1 knockdown inhibited the population doubling (PDL) and treatment of HQ inhibited the PDL further, as well as the change of ROS. Finally, we discovered that pathways including cyclin D1, NRF2, Rb and pRb, CHK2, and p53, were involved in cell proliferation inhibition with HQ. Taken together, our findings demonstrated that crosstalk between ROS and PARP-1 involves in the cell proliferation in CR-HFKs, and that inhibition of CR-HFK proliferation with HQ is through modulating G1 cell cycle arrest.

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Generation of high quality of hepatocyte-like cells from induced pluripotent stem cells with Parp1 but lacking c-Myc

Abstract: In conclusion, we demonstrated that Parp1 promoted reprogramming process to generate the high quality of iPSCs, which could be used as a high quality source of hepatocytes.

Cited by 13 publications

References 31 publications

Recent advances in the induced pluripotent stem cell‐based skin regeneration

Recent advances in the induced pluripotent stem cell‐based skin regeneration

Current progress in hepatic tissue regeneration by tissue engineering

Crosstalk of moderate ROS and PARP‐1 contributes to sustainable proliferation of conditionally reprogrammed keratinocytes

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