Generation of free radical by interaction of iron with thiols in human plasma and its possible significance

Chung, Ka-Young; Lee, Seung-Jin; Chung, Seung-Min; Lee, Moo-Yeol; Bae, Ok-Nam; Chung, Jin‐Ho

doi:10.1016/j.thromres.2004.11.021

Cited by 27 publications

(17 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Albeit not completely understood, we believe that thiols are oxidized (consumed/used) in these subjects due to OS status following iron overload. Another possibility is that the iron could react non-enzymatically with thiols in plasma to generate ROS, which directly leads to reduction of antioxidant capacity in plasma and the increased susceptibility of blood components to oxidation [35] . Thus, this thiol-dependent free radical generation by iron overload might be a potential contributing factor for the changes in the oxidative markers reported here.…”

Section: Fernandes Ms Et Al Biochemical Markers In Polytransfused Smentioning

confidence: 99%

Oxidative stress and labile plasmatic iron in anemic patients following blood therapy

Fernandes

Rissi

Zuravski

et al. 2014

WJEM

View full text Add to dashboard Cite

AIM:To determine the plasmatic iron content and evaluate the oxidative stress (OS) markers in subjects receiving blood therapy. METHODS:Thirty-nine individuals with unspecified anemia receiving blood transfusions and 15 healthy subjects were included in the study. Anemic subjects were divided into three subgrouP: (1) those that received up to five blood transfusions (n = 14); (2) those that received from five to ten transfusions (n = 11); and (3) those that received more than ten transfusions (n = 14). Blood samples were collected by venous arm puncture and stored in tubes containing heparin. The plasma and cells were separated by centrifugation and subsequently used for analyses. Statistical analyses were performed using Kruskal-Wallis analysis of variance followed by Dunn's multiple comparison tests when appropriate. RESULTS:The eletrophoretic hemoglobin profiles of the subjects included in this study indicated that no patients presented with hemoglobinopathy. Labile plasmatic iron, ferritin, protein carbonyl, thiobarbituric acidreactive substances (TBARS) and dichlorofluorescein diacetate oxidation were significantly higher (P < 0.05), whereas total thiol levels were significantly lower (P < 0.05) in transfused subjects compared to controls. Additionally, the activity of catalase, superoxide dismutase and glutathione peroxidase were significantly lower in the transfused subjects (P < 0.05). Antioxidant enzyme activities and total thiol levels were positively correlated (P < 0.05), and negatively correlated with the levels of protein carbonyl and TBARS (P < 0.05). In contrast, protein carbonyl and TBARS were positively correlated (P < 0.05). Altogether, these data confirm the involvement of OS in patients following therapy with repeated blood transfusions. CONCLUSION:Our data reveal that changes in OS markers are correlated with levels of labile plasmatic iron and ferritin and the number of transfusions.

show abstract

Section: Fernandes Ms Et Al Biochemical Markers In Polytransfused Smentioning

confidence: 99%

Oxidative stress and labile plasmatic iron in anemic patients following blood therapy

Fernandes

Rissi

Zuravski

et al. 2014

WJEM

View full text Add to dashboard Cite

show abstract

“…Some hippocampal changes appeared after 4 weeks of the event, with increased astrocyte density and cell loss in the CA1 area (Bennett et al, 1998;Farkas et al, 2004;Pappas et al, 1996;Vicente et al, 2009). The brain tissue is sensitive to oxidative imbalance and previous studies have demonstrated that oxidative injury plays a key role in the pathogenesis of numerous neurodegenerative diseases including stroke, Alzheimer's disease and vascular dementia (Chung et al, 2005;Coyle and Puttfarcken, 1993). Oxidative stress is defined as the imbalance between oxidants and antioxidants in favor of oxidant activity that potentially leads to tissue damage (Polidori et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Environmental enrichment prevents behavioral deficits and oxidative stress caused by chronic cerebral hypoperfusion in the rat

et al. 2012

View full text Add to dashboard Cite

show abstract

“…The total antioxidant activity of the organ samples was measured by using the oxygen radical absorbance capacity (ORAC) assay according to Chung et al (2005). This assay was carried out in black-walled, 96-well plates at 37°C.…”

Section: Oxygen Radical Absorbance Capacity (Orac) Assaymentioning

confidence: 99%

The safety of Osmanthus fragrans ethanol extract treatment in BALB/c mice

Hung¹,

Lin

et al. 2013

International Journal of Pharmacology and Toxicology

View full text Add to dashboard Cite

Background: Osmanthus fragrans flower, recently certificated as a new natural antioxidant, has been used in various foods. We performed an acute and subacute toxicological test for evaluating the safety and toxicity of 75 % ethanol extraction of Osmanthus fragrans (OFEE). Method: In the acute toxicity study, a single dose of 5 g/kg and 10 g/kg of the extract was administered orally to six mice. General behaviour, mortality and toxic symptoms were determined daily for 14 days. For the subacute toxicity, two groups of 12 mice received 0.9% normal saline (control) and 1 g/kg of the extract daily for 28 consecutive days by oral gavage. The animals were observed daily for abnormal clinical signs and death. Body weight, haematological and biochemical parameters of blood as well as kidney, liver, lung and spleen tissues histology were evaluated. Results: The total phenolic contents in OFEE were 371.71 ± 12.35 mg GAE/g extracts and total flavonoid contents were 47.23 ± 5.36 mg QE/g extract. OFEE did not cause any mortality or morbidity. Maximum tolerated dose (MTD) was 10 g/kg body weight in our BALB/c mice, which can be regarded as virtually non-toxic. Administration of OFEE at dose 1 g/kg/day for 28-day did not cause changed in hematological, biochemical assay, and histopathological conditions change, suggesting a no-observed-adverse-effect level (NOVEL) of 1 g/kg/day. Conclusion: We found no evidence of adverse effects in our laboratory test and pathological studies. 75 % ethanol extraction may safely be used in the research of Osmanthus fragrans in animal and probably human studies.

show abstract

Generation of free radical by interaction of iron with thiols in human plasma and its possible significance

Cited by 27 publications

References 42 publications

Oxidative stress and labile plasmatic iron in anemic patients following blood therapy

Oxidative stress and labile plasmatic iron in anemic patients following blood therapy

Environmental enrichment prevents behavioral deficits and oxidative stress caused by chronic cerebral hypoperfusion in the rat

The safety of Osmanthus fragrans ethanol extract treatment in BALB/c mice

Contact Info

Product

Resources

About