Generation of Fertile Cloned Rats by Regulating Oocyte Activation

Zhou, Qi; Renard, Jean‐Paul; Friec, Gaëlle Le; Brochard, Vincent; Beaujean, Nathalie; Chérifi, Yacine; Fraichard, Alexandre; Cozzi, J.

doi:10.1126/science.1088313

Cited by 386 publications

(226 citation statements)

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“…Gene targeting has many useful applications in science, medicine and industry and may be one of the most useful applications of somatic cell cloning technology. Currently, gene targeting using ES cells has been successful only in mice, but somatic cell cloning has been successful for many species [21][22][23][24][25] . The results obtained in this study indicate that complex genetic modifications can now be readily made for a wide variety of genes in many species.…”

Section: Discussionmentioning

confidence: 99%

Sequential targeting of the genes encoding immunoglobulin-μ and prion protein in cattle

et al. 2004

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Gene targeting is accomplished using embryonic stem cells in the mouse but has been successful, only using primary somatic cells followed by embryonic cloning, in other species. Gene targeting in somatic cells versus embryonic stem cells is a challenge; consequently, there are few reported successes and none include the targeting of transcriptionally silent genes or double targeting to produce homozygotes. Here, we report a sequential gene targeting system for primary fibroblast cells that we used to knock out both alleles of a silent gene, the bovine gene encoding immunoglobulin-µ (IGHM), and produce both heterozygous and homozygous knockout calves. We also carried out sequential knockout targeting of both alleles of a gene that is active in fibroblasts, encoding the bovine prion protein (PRNP), in the same genetic line to produce doubly homozygous knockout fetuses. The sequential gene targeting system we used alleviates the need for germline transmission for complex genetic modifications and should be broadly applicable to gene functional analysis and to biomedical and agricultural applications.

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Section: Discussionmentioning

confidence: 99%

Sequential targeting of the genes encoding immunoglobulin-μ and prion protein in cattle

et al. 2004

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“…However, the rat SSC system has several important features. First, it is likely to be faster and more efficient than the recently described nuclear transplantation approach (34). Second, the doubling time for in vitro rat SSC replication probably can be reduced from Ϸ11 days to 5-6 days, the doubling time required for mouse SSCs, because transplanted rat SSCs support donorderived spermatogonial colony expansion at more than twice the rate found for mouse SSCs (35).…”

Section: Discussionmentioning

confidence: 99%

Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat

Ryu

Kubota

Avarbock

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

252

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Self-renewal of spermatogonial stem cells (SSCs) is the foundation for maintenance of spermatogenesis throughout life in males and for continuation of a species. The molecular mechanism underlying stem cell self-renewal is a fundamental question in stem cell biology. Recently, we identified growth factors necessary for self-renewal of mouse SSCs and established a serum-free culture system for their proliferation in vitro. To determine whether the stimulatory signals for SSC replication are conserved among different species, we extended the culture system to rat SSCs. Initially, a method to assess in vitro expansion of SSCs was developed by using flow cytometric analysis, and, subsequently, we found that a combination of glial cell line-derived neurotrophic factor, soluble glial cell line-derived neurotrophic factor-family receptor ␣-1 and basic fibroblast growth factor supports proliferation of rat SSCs. When cultured with the three factors, stem cells proliferated continuously for >7 months, and transplantation of the cultured SSCs to recipient rats generated donor stem cell-derived progeny, demonstrating that the cultured stem cells are normal. The growth factor requirement for replication of rat SSCs is identical to that of mouse; therefore, the signaling factors for SSC self-renewal are conserved in these two species. Because SSCs from many mammals, including human, can replicate in mouse seminiferous tubules after transplantation, the growth factors required for SSC self-renewal may be conserved among many different species. Furthermore, development of a long-term culture system for rat SSCs has established a foundation for germ-line modification of the rat by gene targeting technology.germ-line stem cell ͉ spermatogenesis ͉ testis ͉ glial cell line-derived neurotrophic factor

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“…[449][450][451][452][453] Following the sequencing of the Brown Norwegian rat genome, it should be possible to exploit what we have learned from the mouse and relate it to this more physiologically, pharmacologically, toxicologically and pathologically significant animal. Recent breakthroughs in controlling egg activation 454 have in addition paved the way for the development of genetically manipulated rats. Combining the use of such models should offer more pertinent preclinical data, thus reducing current expenditure on drug development and afford greater clinical success.…”

Section: Future Perspectivesmentioning

confidence: 99%

Animal models of acute myelogenous leukaemia – development, application and future perspectives

2005

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From the early inception of the transplant models through to contemporary genetic and xenograft models, evolution of murine leukaemic model systems have been critical to our general comprehension and treatment of cancer, and, more specifically, disease states such as acute myelogenous leukaemia (AML). However, even with modern advances in therapeutics and molecular diagnostics, the majority of AML patients die from their disease. Thus, in the absence of definitive in vitro models which precisely recapitulate the in vivo setting of human AMLs and failure of significant numbers of new drugs late in clinical trials, it is essential that murine AML models are developed to exploit more specific, targeted therapeutics. While various model systems are described and discussed in the literature from initial transplant models such as BNML and spontaneous murine leukaemia virus models, to the more definitive genetic and clinically significant NOD/SCID xenograft models, there exists no single compendium which directly assesses, reviews or compares the relevance of these models. Thus, the function of this article is to provide clinicians and experimentalists a chronological, comprehensive appraisal of all AML model systems, critical discussion on the elucidation of their roles in our understanding of AML and consideration to their efficacy in the development of AML chemotherapeutics.

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Generation of Fertile Cloned Rats by Regulating Oocyte Activation

Cited by 386 publications

References 5 publications

Sequential targeting of the genes encoding immunoglobulin-μ and prion protein in cattle

Sequential targeting of the genes encoding immunoglobulin-μ and prion protein in cattle

Conservation of spermatogonial stem cell self-renewal signaling between mouse and rat

Animal models of acute myelogenous leukaemia – development, application and future perspectives

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