Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells

Shen, Mengcheng; Li, Chun; Wu, Joseph C.

doi:10.1007/978-1-0716-1979-7_15

Cited by 6 publications

(12 citation statements)

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“…We found that temporal activation or inhibition of key morphogens at different differentiation stages enabled the generation of pure EPIs (Figure [B, i]). 2 In contrast, using an extant protocol that only manipulated Wnt signaling throughout the entire differentiation process (referred to as the GiWiGi protocol ), 3 we observed a large variation of EPI induction efficiency (ranging from 3% to 87%) even among iterative differentiations (Figure [B, ii]). Moreover, our stepwise protocol generated more mature EPIs ( ALDH1A2 [aldehyde dehydrogenase 1 family member A2], UPK3B [uroplakin 3B],and ANXA8 [annexin A8]) than did the GiWiGi protocol (Figure [C]).…”

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confidence: 93%

Stepwise Generation of Human Induced Pluripotent Stem Cell–Derived Cardiac Pericytes to Model Coronary Microvascular Dysfunction

et al. 2023

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confidence: 93%

Stepwise Generation of Human Induced Pluripotent Stem Cell–Derived Cardiac Pericytes to Model Coronary Microvascular Dysfunction

et al. 2023

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“…Vascular tree has a variable embryonic origin ( Figure 2 ) ( 14 – 16 ). Embryonic fate-mapping studies have demonstrated that the aortic root, ascending aorta and aortic arch are populated by vascular smooth muscle cells (VSMCs) arising from NC, whereas VSMCs from the paraxial mesoderm or somites populate the descending aorta ( 17 , 18 ). Studies have shown that CNS cells also contribute to the VSMCs found in some of the branchial arch arteries ( 19 ).…”

Section: Resultsmentioning

confidence: 99%

“…NCCs are multipotent, capable of differentiating into various cells in our bodies ( 18 , 44 ). The multipotent capacity of the NCC are attributed to the combination of complex multimodal gene regulatory mechanism ( 44 ).…”

Section: Resultsmentioning

confidence: 99%

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Systematic review of cardiovascular neurocristopathy—contemporary insights and future perspectives

Soliman,

Acharya,

Gilard

et al. 2024

Front. Cardiovasc. Med.

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IntroductionNeural crest cells (NCCs) are multipotent and are attributed to the combination of complex multimodal gene regulatory mechanisms. Cardiac neural crest (CNC) cells, originating from the dorsal neural tube, are pivotal architects of the cardio-neuro-vascular domain, which orchestrates the embryogenesis of critical cardiac and vascular structures. Remarkably, while the scientific community compiled a comprehensive inventory of neural crest derivatives by the early 1980s, our understanding of the CNC's role in various cardiovascular disease processes still needs to be explored. This review delves into the differentiation of NCC, specifically the CNC cells, and explores the diverse facets of non-syndromic cardiovascular neurocristopathies.MethodsA systematic review was conducted as per the PRISMA Statement. Three prominent databases, PubMed, Scopus, and Embase, were searched, which yielded 1,840 studies. We excluded 1,796 studies, and the final selection of 44 studies formed the basis of this comprehensive review.ResultsNeurocristopathies are a group of genetic disorders that affect the development of cells derived from the NC. Cardiovascular neurocristopathy, i.e., cardiopathy and vasculopathy, associated with the NCC could occur in the form of (1) cardiac septation disorders, mainly the aortico-pulmonary septum; (2) great vessels and vascular disorders; (3) myocardial dysfunction; and (4) a combination of all three phenotypes. This could result from abnormalities in NCC migration, differentiation, or proliferation leading to structural abnormalities and are attributed to genetic, familial, sporadic or acquired causes.DiscussionPhenotypic characteristics of cardiovascular neurocristopathies, such as bicuspid aortic valve and thoracic aortic aneurysm, share a common embryonic origin and are surprisingly prevalent in the general population, necessitating further research to identify the underlying pathogenic and genetic factors responsible for these cardiac anomalies. Such discoveries are essential for enhancing diagnostic screening and refining therapeutic interventions, ultimately improving the lives of individuals affected by these conditions.

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“…The purity of intermediate cell types at each stage, including mid-primitive streak (MPS), lateral plate mesoderm (LPM), splanchnic mesoderm (SM), septum transversum (ST), and EPI, can be confirmed by immunofluorescence staining of cell type-specific markers. 2 The induction media for intermediate cell types at different stages should be prepared according to the tables in the “ materials and equipment ” section. Note: While all stage-specific induction media can be stored at 4°C for up to a week, it is advisable to prepare them fresh for best results.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Protocol to generate cardiac pericytes from human induced pluripotent stem cells

Shen¹,

Zhao²,

Khokhar³

et al. 2023

STAR Protocols

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Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells

Cited by 6 publications

References 30 publications

Stepwise Generation of Human Induced Pluripotent Stem Cell–Derived Cardiac Pericytes to Model Coronary Microvascular Dysfunction

Stepwise Generation of Human Induced Pluripotent Stem Cell–Derived Cardiac Pericytes to Model Coronary Microvascular Dysfunction

Systematic review of cardiovascular neurocristopathy—contemporary insights and future perspectives

Protocol to generate cardiac pericytes from human induced pluripotent stem cells

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