Generation of double emulsions from commercial single-emulsion microfluidic chips: a quality-control study

Lin, Siying; Mao, Lingjing; Ying, Jiaxu; Berthet, Nicolás; Zhou, Jia; Riaud, Antoine

doi:10.1007/s10404-022-02575-7

Cited by 4 publications

(7 citation statements)

References 26 publications

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“…The droplets are generated using a microfluidic chip (Fluidic 537, Chipshop, Germany), with the continuous (outer phase) phase being HFE-7500 oil with 1% PEG-Krytox surfactant (Emulseo, France) and the dispersed (inner) phase being deionized water. 40 When required, fluorescent nanoparticles (RF100C, provided by Huge Biotechnology) are introduced in the dispersed phase at a concentration of 2%v/v. Experimental Section.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Water droplets were generated using Fluidic 537 microfluidic chips, resulting in a highly monodispersed emulsion. 40 The oil flow rate was 10 μL/ min, and the water flow rate was 3 μL/min. The typical droplet size is 50 μm, but we used Ostwald ripening (keeping the droplets 60 days in a fridge at 3 °C) to broaden the droplet size distribution from a diameter of 25 to 90 μm.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…According to the manufacturer, this oil has a saturating vapor pressure of 0.28 kPa at 25 °C, which is 10 times lower than that of water (3.2 kPa), and therefore does not evaporate much during the experiments. The droplets are generated using a microfluidic chip (Fluidic 537, Chipshop, Germany), with the continuous (outer phase) phase being HFE-7500 oil with 1% PEG-Krytox surfactant (Emulseo, France) and the dispersed (inner) phase being deionized water . When required, fluorescent nanoparticles (RF100C, provided by Huge Biotechnology) are introduced in the dispersed phase at a concentration of 2%v/v.…”

Section: Methodsmentioning

confidence: 99%

“…Water droplets were generated using Fluidic 537 microfluidic chips, resulting in a highly monodispersed emulsion . The oil flow rate was 10 μL/min, and the water flow rate was 3 μL/min.…”

Section: Methodsmentioning

confidence: 99%

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Selective Sparse Sampling of Water Droplets in Oil with Acoustic Tweezers

Lin,

Riaud,

Zhou

2024

ACS Sens.

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In microfluidics, water droplets are often used as independent biochemical microreactor units, enabling the implementation of massively parallel screening assays where only a few of the reacting water droplets yield a positive result. However, sampling the product of these few successful reactions is an unsolved challenge. One possible solution is to use acoustic tweezers, which are lab-free, easily miniaturized, and biocompatible manipulation tools, and existing acoustic tweezers manipulating particles or cells, and water droplet manipulation in oil with an acoustic tweezer is absent. The first challenge in attempting to recover a few water droplets from a large batch is the selective manipulation of water droplets in an oil system. In this paper, we trap and manipulate single water droplets in oil using integrated single-beam (focused beam/vortex beam) acoustic tweezers for the first time. We find that water droplets with a diameter smaller than half a wavelength are trapped by acoustic vortices, while larger ones are better captured by focused acoustic beams. It is the first step to extract the target water droplet microreactors (positive ones) in an oil system and analyze their content. Compared to previous techniques, such as fluorescence-activated cell sorting (FACS), our technique is sparse, meaning that the sampling time is proportional to the number of droplets required and very insensitive to the total number of microreactors, making it well suited for large-scale screening assays.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Water droplets were generated using Fluidic 537 microfluidic chips, resulting in a highly monodispersed emulsion . The oil flow rate was 10 μL/min, and the water flow rate was 3 μL/min.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Selective Sparse Sampling of Water Droplets in Oil with Acoustic Tweezers

Lin,

Riaud,

Zhou

2024

ACS Sens.

Self Cite

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“…[30,[49][50][51][52][53][54][55][56][57] Interestingly, recent works have shown that vesicles can be produced from double (water-in-oilin-water droplets) emulsions. [58][59][60][61][62][63] These were prepared by microfluidics and this allows encapsulating cargoes in their lumen. The double emulsion initially produced evolves upon dewetting into an oil droplet and a lipid vesicle, in which cargoes are entrapped.…”

Section: Introductionmentioning

confidence: 99%

Double Emulsion Droplets as a Plausible Step to Fatty Acid Protocells

Douliez

2023

Small Methods

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It is assumed that life originated on the Earth from vesicles made of fatty acids. These amphiphiles are the simplest chemicals, which can be present in the prebiotic soup, capable of self‐assembling into compartments mimicking modern cells. Production of fatty acid vesicles is widely studied, as their growing and division. However, how prebiotic chemicals require to further yield living cells encapsulated within protocells remains unclear. Here, one suggests a scenario based on recent studies, which shows that phospholipid vesicles can form from double emulsions affording facile encapsulation of cargos. In these works, water‐in‐oil‐in‐water droplets are produced by microfluidics, having dispersed lipids in the oil. Dewetting of the oil droplet leaves the internal aqueous droplet covered by a lipid bilayer, entrapping cargos. In this review, formation of fatty acid protocells is briefly reviewed, together with the procedure for preparing double emulsions and vesicles from double emulsion and finally, it is proposed that double emulsion droplets formed in the deep ocean where undersea volcano expulsed materials, with fatty acids (under their carboxylic form) and alkanols as the oily phase, entrapping hydrosoluble prebiotic chemicals in a double emulsion droplet core. Once formed, double emulsion droplets can move up to the surface, where an increase of pH, variation of pressure and/or temperature may have allowed dewetting of the oily droplet, leaving a fatty acid vesicular protocell with encapsulated prebiotic materials.

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