Generation of diverse neuronal subtypes in cloned populations of stem-like cells

Varga, Balázs; Hádinger, Nóra; Gócza, Elen; Dulberg, Vered; Demeter, Kornél; Madarász, Emı́lia; Herberth, Balázs

doi:10.1186/1471-213x-8-89

Cited by 37 publications

(44 citation statements)

References 58 publications

(66 reference statements)

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“…For this purpose, neural stem cells cloned from early embryonic (E9) mouse neuroectoderm (NE-4C cells; [29][30][31][32][33][34]) and stem/progenitor cells isolated from late embryonic (E14.5) or adult (P62) neurogenic zones (RGl cells; [35]) were used. The results demonstrated that the investigated stem/ progenitor populations showed significant differences in many aspects of both RA metabolism and retinoid responsiveness.…”

Section: Introductionmentioning

confidence: 99%

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Orsolits

Borsy

Madarász

et al. 2013

Stem Cells and Development

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Section: Introductionmentioning

confidence: 99%

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Orsolits

Borsy

Madarász

et al. 2013

Stem Cells and Development

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“…NE-4C line is shown to differentiate into neurons with appropriate inducing factors after 4 d of culture [35]. The biphasic electrical stimulation was applied continuously during 4 d of culture to see its effect on the early stage of neural differentiation without introducing exogenous retinoic acid in the culture media.…”

Section: Electrical Stimulation and Nscs Differentiationmentioning

confidence: 99%

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

et al. 2018

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Objective. Nanomaterials, such as carbon nanotubes (CNTs), have been introduced to modify the surface properties of scaffolds, thus enhancing the interaction between the neural cells and biomaterials. In addition to superior electrical conductivity, CNTs can provide nanoscale structures similar to those present in the natural neural environment. The primary objective of this study is to investigate the proliferative capability and differential potential of neural stem cells (NSCs) seeded on a CNT incorporated scaffold. Approach. Amine functionalized multi-walled carbon nanotubes (MWCNTs) were incorporated with a PEGDA polymer to provide enhanced electrical properties as well as nanofeatures on the surface of the scaffold. A stereolithography 3D printer was employed to fabricate a well-dispersed MWCNT-hydrogel composite neural scaffold with a tunable porous structure. 3D printing allows easy fabrication of complex 3D scaffolds with extremely intricate microarchitectures and controlled porosity. Main results. Our results showed that MWCNT-incorporated scaffolds promoted neural stem cell proliferation and early neuronal differentiation when compared to those scaffolds without the MWCNTs. Furthermore, biphasic pulse stimulation with 500 µA current promoted neuronal maturity quantified through protein expression analysis by quantitative polymerase chain reaction. Significance. Results of this study demonstrated that an electroconductive MWCNT scaffold, coupled with electrical stimulation, may have a synergistic effect on promoting neurite outgrowth for therapeutic application in nerve regeneration.

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“…5A). To broaden our viewpoint of Lin28a/b functional mechanisms, we sought to identify Lin28a-associated proteins in NE-4C cells, a cell line established from the cerebral vesicle of E9.0 mouse embryos and which mimics NPC proliferation and differentiation in vitro (Varga et al, 2008). We generated a genetically modified Lin28a bacterial artificial chromosome (BAC) vector that encodes a Lin28a fusion protein containing two N-terminal tags: EGFP and Flag (supplementary material Fig.…”

Section: Lin28a Interacts With Imp1 To Regulate Npc Proliferationmentioning

confidence: 99%

Lin28 promotes the proliferative capacity of neural progenitor cells in brain development

et al. 2015

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Neural progenitor cells (NPCs) have distinct proliferation capacities at different stages of brain development. Lin28 is an RNA-binding protein with two homologs in mice: Lin28a and Lin28b. Here we show that Lin28a/b are enriched in early NPCs and their expression declines during neural differentiation. Lin28a single-knockout mice show reduced NPC proliferation, enhanced cell cycle exit and a smaller brain, whereas mice lacking both Lin28a alleles and one Lin28b allele display similar but more severe phenotypes. Ectopic expression of Lin28a in mice results in increased NPC proliferation, NPC numbers and brain size. Mechanistically, Lin28a physically and functionally interacts with Imp1 (Igf2bp1) and regulates Igf2-mTOR signaling. The function of Lin28a/b in NPCs could be attributed, at least in part, to the regulation of their mRNA targets that encode Igf1r and Hmga2. Thus, Lin28a and Lin28b have overlapping functions in temporally regulating NPC proliferation during early brain development.

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Generation of diverse neuronal subtypes in cloned populations of stem-like cells

Cited by 37 publications

References 58 publications

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

3D printing nano conductive multi-walled carbon nanotube scaffolds for nerve regeneration

Lin28 promotes the proliferative capacity of neural progenitor cells in brain development

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